Field Cold Resistance Identification and Comprehensive Evaluation of Different Pennisetum Resources
SUN Qi-lu1, MA Jie-yu1, PENG Yan1, NIE Gang1, QIONG Da2, ZHANG Xin-quan1
Affiliations
1 College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 610000, China; 2. Agricultural Technology Extension Station, Agriculture, Rural Affairs, Science, Technology and Water Resources Bureau of Gerze County, Ngari Prefecture, Gerze, Xizang 859200, China
To screen cold-tolerant germplasm resources of Pennisetum, clarify the cold resistance differences among different germplasms, and provide a theoretical basis for cold resistance breeding, 118 Pennisetum germplasm resources were used as experimental materials in this study, and comprehensive evaluation of cold resistance was carried out via field low-temperature stress treatment combined with eight cold resistance-related indicators. The results revealed that the cold resistance-related traits of the tested germplasms possessed abundant genetic diversity, which could provide sufficient genetic basis for cold resistance breeding. Chlorophyll content was significantly correlated with regrowth seedling number and stem sugar content, serving as key factors affecting cold resistance differences among Pennisetum germplasms. Principal component analysis simplified eight indicators into four principal components with a cumulative contribution rate of 80.924%, with chlorophyll reduction value after low-temperature stress, chlorophyll content under low temperature, regrowth seedling number and stem sugar content identified as the core indicators. Based on cluster analysis, all the germplasms were classified into three groups, and a total of 30 excellent cold-resistant germplasms including Pp20230630-3, Pp20230628-5 and Pp014 were screened out using membership function method. This study provides important germplasm resources and theoretical references for cold-resistant germplasm innovation and new variety breeding of Pennisetum.
SUN Qi-lu, MA Jie-yu, PENG Yan, NIE Gang, QIONG Da, ZHANG Xin-quan.
Field Cold Resistance Identification and Comprehensive Evaluation of Different Pennisetum Resources[J].
Acta Agrestia Sinica,
2026
, 34
(9)
: 3365
-3373
.
DOI: 10.11733/j.issn.1007-0435.2026.09.020
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FARRELL G,SIMONS S A,HILLOCKS R J. Aspects of the biology of Ustilago kamerunensis,a smut pathogen of Napier grass (Pennisetum purpureum)[J]. Journal of Phytopathology,2001,149(11/12):739-744 BRITO DA SILVA V,DAHER R,DE SOUZA Y P,et al. Assessment of energy production in full-sibling families of elephant grass by mixed models[J]. Renewable Energy,2020,146:744-749 TAN W B,QIN P L. Analysis on the contents of routine nutritive components and gross energy of Pennisetum purpureum [J]. Journal of Southwest Forestry College,2008(3):45-47 谭文彪,覃培龙. 象草常规营养成分及总能含量分析[J]. 西南林学院学报,2008(3):45-47 WOODARD K R,PRINE G M,BACHREIN S. Solar energy recovery by elephantgrass,energycane,and elephantmillet canopies[J]. Crop Science,1993,33(4):824-830 YUAN F J,ZHOU Z W,XU Y F,et al. Effect of planting method and row spacing on the productivity of Pennisetum purpureum[J]. Pratacultural Science,2008,25(2):73-76 袁福锦,周自玮,许艳芬,等. 栽种对象草生产性能的作用[J]. 草业科学,2008,25(2):73-76 GEZAHAGN K B,FEKEDE F,GETNET A,et al.Agronomic performance and dry matter yield stability of Napier grass (Pennisetum purpureum) genotypes in Southwestern Ethiopia[J]. Agronomy Journal homepage,2024,116(5)2304-2317 WEN X N,JIAN Y Z,XIE X M. The comprehensive exploitation and utilization of Pennisetum purpureum[J]. Pratacultural Science,2009,26(9):108-112 温晓娜,简有志,解新明. 象草资源的综合开发利用[J]. 草业科学,2009,26(9):108-112 ZHANG X,GU H R,DING C L,et al. Path coefficient and cluster analyses of yield and morphological traits in Pennisetum purpureum[J]. Tropical Grasslands,2010,44(2):95-102 FERRARIS R. The effect of photoperiod and temperature on the first crop and ratoon growth of Pennisetum purpureum Schum[J]. Australian Journal of Agricultural Research,1978,29(5):941-950 BORRAJO C I. Caracteres morfo-fisiológicos que definen la producción de forraje en agropiro bajo estrés por sequía y salinidad[J] Revista Argentina de Producción Animal,2025,44(S1):317 PEREIRA A V,LIRA M A,MACHADO J C,et al. Elephantgrass,a tropical grass for cutting and grazing[J]. Revista Brasileira de Ciências Agrárias - Brazilian Journal of Agricultural Sciences,2021,16(3):e9317 MA T,LI X Q,GAO L.Exogenous strigolactone regulates AsA-GSH cycle and hormone metabolism of Pennisetum purpureum to adapt to low temperature stress[J/OL]. https://link.cnki.net/urlid/62.1069.S.20251106.1405.009,2025-11-06/2026-02-04 马婷,李修清,高利. 外源独脚金内酯调控象草AsA-GSH循环和激素代谢来适应低温胁迫[J/OL]. https://link.cnki.net/urlid/62.1069.S.20251106.1405.009,2025-11-06/2026-02-04 BURNER D M,HALE A L,VIATOR R P, et al. Ratoon cold tolerance of Pennisetum,Erianthus,and Saccharum bioenergy feedstocks[J]. Industrial Crops and Products,2017,109:327-334 GAO S B,MO L D,ZHANG L H,et al. Phenotypic plasticity vs. local adaptation in quantitative traits differences of Stipa grandis in semi-arid steppe,China[J]. Scientific Reports,2018,8:3148 MENG A J,WEN D X,ZHANG C Q. Maize seed germination under low-temperature stress impacts seedling growth under normal temperature by modulating photosynthesis and antioxidant metabolism[J]. Frontiers in Plant Science,2022,13:843033 ZHAO Y Q,HAN Q H,DING C B,et al. Effect of low temperature on chlorophyll biosynthesis and chloroplast biogenesis of rice seedlings during greening[J]. International Journal of Molecular Sciences,2020,21(4):1390 GRIMAUD F,RENAUT J,DUMONT E,et al. Exploring chloroplastic changes related to chilling and freezing tolerance during cold acclimation of pea (Pisum sativum L.)[J]. Journal of Proteomics,2013,80:145-159 LU J Y,TIAN H,XIONG J B,et al. A multi-trait evaluation of cold resistance of 14 native Pennisetum alopecuroides germplasm lines at the seedling stage [J]. Acta Prataculturae Sinica,2024,33(8):98-111 陆姣云,田宏,熊军波,等. 14份乡土狼尾草材料幼苗的耐冷性综合评价[J]. 草业学报,2024,33(8):98-111 FU B C,BO W. Analyses of the subordinate function and the principal components of Hosta drought resistance[J]. Acta Agrestia Sinica,2014,22(6):1324-1330 付宝春,薄伟. 玉簪抗旱性隶属函数及主成分分析[J]. 草地学报,2014,22(6):1324-1330 ZHAO G D,ZHAO T S,LI C M,et al. Comprehensive evaluation on cold resistance of eleven wild apple rootstocks[J]. Journal of Northwest Forestry University,2018,33(6):145-151 赵国栋,赵同生,李春敏,等. 11个苹果野生砧木品种低温处理抗性指标的综合评价[J]. 西北林学院学报,2018,33(6): 145-151 DUAN W,MUNIRE M J T,JIAMILIGULI Y M, et al. Physiological responses to low-temperature stress and cold resistance evaluation of 20 Medicago falcata germplasms [J]. Acta Agrestia Sinica,2026,34(2):611-623 段伟,穆妮热·米吉提,加米丽古丽·伊明,等. 20份黄花苜蓿种质低温胁迫生理响应及抗寒性评价[J]. 草地学报, 2026,34(2):611-623 SUN W Y,LÜ Y,LI Y C,et al. Comprehensive evaluation of cold resistance of 21 wild Elymus germplasms in Xizang[J]. Pratacultural Science,2025,12(12):3141-3152 孙婉玉,吕晔,李阳晨,等. 21份西藏野生披碱草属种质抗寒性综合评价[J]. 草业科学, 2025, 12(12): 3141-3152 WANG R F,LI X Z,LI Q C,et al. Effects of different concentrations of silicon on the cold resistance of Elymus sibiricus seedlings [J]. Acta Prataculturae Sinica,2026,35(1):130-139 王若非,李昕哲,李祺策,等. 不同浓度的硅处理对老芒麦苗期抗寒性的影响[J]. 草业学报,2026,35(1):130-139 DONG H X,GREEN S V,NISHIWAKI A,et al. Winter hardiness of Miscanthus (I): Overwintering ability and yield of new Miscanthus×giganteus genotypes in Illinois and Arkansas[J]. GCB Bioenergy,2019,11(5):691-705 BRAR A,SANDHU S K,SHARMA S. Physiological and biochemical traits as indicators for cold tolerance in sugarcane (Saccharum species)[J]. Agricultural Research Journal,2018,55(2):210 CHAUHAN H,KHURANA N,NIJHAVAN A,et al. The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress[J]. Plant,Cell & Environment,2012,35(11):1912-1931 LI J,BAI X,RAN F,et al. Effects of combined extreme cold and drought stress on growth,photosynthesis,and physiological characteristics of cool-season grasses[J]. Scientific Reports,2024,14:116 LIANG K L,JIA C Z,SUN J H,et al. Evaluation of physiological responses and tolerance to low-temperature stress of ten Elymus nutans varieties [J]. Acta Prataculturae Sinica,2019,28(3):111-121 梁坤伦,贾存智,孙金豪,等. 高寒地区垂穗披碱草种质对低温胁迫的生理响应及其耐寒性评价[J]. 草业学报,2019, 28(3):111-121 MA X X,NAN L L,WANG L Q,et al. Responses of seedlings from new alfalfa lines to low temperature stress[J]. Chinese Journal of Grassland,2025,47(5):66-77 马香香,南丽丽,王利群,等. 紫花苜蓿新品系幼苗对低温胁迫的响应[J]. 中国草地学报,2025,47(5):66-77 BURNER D M,HALE A L,CARVER P,et al. Biomass yield comparisons of giant Miscanthus,giant reed,and miscane grown under irrigated and rainfed conditions[J]. Industrial Crops and Products,2015,76:1025-1032 FONTEYNE S. Evaluation of chilling and frost stress tolerance in miscanthus : from winter survival and early-season growth to final biomass yield[D]. Ghent: Ghent University,2017:1-2 GUO H L,LIU J X,ZHU X H,et al. Evaluation of cold resistance of Zoysia hybrids [J]. Acta Agrestia Sinica,2006,14(1):24-28 郭海林,刘建秀,朱雪花,等. 结缕草属杂交后代抗寒性评价[J]. 草地学报,2006,14(1):24-28 ERGON Å ,AMDAHL H. Winter survival in red clover: experimental evidence for interactions among stresses[J]. BMC Plant Biology ,2024,24(1):467 DONG S H,JIA M,ZHANG H,et al. The physiological response and tolerance evaluation of tetraploid Carex breviculmis to low temperature [J]. Acta Agrestia Sinica,2024,32(7):2089-2098 董双慧,贾明,张辉,等. 四倍体青绿苔草对低温的生理响应及耐寒性评价[J]. 草地学报,2024,32(7):2089-2098 WANG M,LI L,JIA R,et al. Evaluation of physiological characteristics and cold resistance of 10 alfalfa varieties under low temperature stress[J]. Acta Prataculturae Sinica,2024,33(6):76-88 王敏,李莉,贾蓉,等. 10种紫花苜蓿在低温胁迫下的生理特性及耐寒性评价[J]. 草业学报,2024,33(6):76-88