Article(id=1309575136005677645, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575113855562513, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.09.013, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1762790400000, receivedDateStr=2025-11-11, revisedDate=1765728000000, revisedDateStr=2025-12-15, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156643152, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156643152, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156643152, creator=13701087609, updateTime=1790156643152, updator=13701087609, issue=Issue{id=1309575113855562513, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='9', pageStart='3141', pageEnd='3524', issueExtLink='null', onlineDate='null', pubDate='1789401600000', pubDateStr='2026-09-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1790156637871, creator='13701087609', updateTime=1790156973247, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576520587374966, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575113855562513, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576520587374967, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575113855562513, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=3284, endPage=3291, ext={EN=ArticleExt(id=1309575137242997328, articleId=1309575136005677645, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Ethephon on Physiological Indicators such as Proline in Turfgrass under Drought Stress, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Appropriate concentrations of ethephon can improve the drought tolerance of turfgrasses, but the specific mechanism of action remains unclear. As an osmotic regulator, proline plays an important role in plant responses to stress. In this study, Kentucky bluegrass (Poa pratensis) and perennial ryegrass (Lolium perenne) were used as materials. We examined the effects of ethephon on physiological indices of turfgrasses in response to drought stress, analyzed changes in the expression of genes related to proline biosynthesis and catabolism, and explored the role of proline in ethephon-improved drought resistance of turfgrasses. The results showed that ethephon alleviated the decrease of relative water content and the increase of electrolyte leakage of turfgrass leaves induced by drought stress, thus contributing to improved drought tolerance. Under drought stress, proline content increased in the two Poa pratensis cultivars, whereas the changes were inconsistent among Lolium perenne cultivars. Ethephon treatment down-regulated the expression of Proline Dehydrogenase (Pro DH) gene in Poa pratensis under drought stress, thereby inhibiting proline degradation. In conclusion, promoting proline accumulation by suppressing the expression of the Pro DH gene might be one of the mechanisms by which ethephon improves drought tolerance in Poa pratensis., authors=TANG Ya-nan1, HAN Lu1, DUAN Meng-qi1, DU Guang-qi1, XU Li-xin1, HAN Lie-bao1, authorsList=TANG Ya-nan, HAN Lu, DUAN Meng-qi, DU Guang-qi, XU Li-xin, HAN Lie-bao, authorCompany=1 School of Grassland Science of Beijing Forestry University, Beijing 100083, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575136932618831, articleId=1309575136005677645, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=乙烯利对草坪草干旱胁迫下脯氨酸等生理指标的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=适宜浓度的乙烯利可以提升草坪草的耐旱性,但具体作用机制未知。脯氨酸作为渗透调节物质,在植物逆境胁迫响应中发挥重要作用。本文以草地早熟禾(Poa pratensis)和多年生黑麦草(Lolium perenne)为材料,检测乙烯利对干旱胁迫下草坪草生理指标的影响,并分析脯氨酸合成与降解代谢相关基因表达的变化,探讨脯氨酸在乙烯利提升草坪草抗旱性中的作用。结果表明:乙烯利可缓解干旱胁迫诱导的草坪草叶片相对含水量的下降和电解质渗透率的上升,对耐旱性提升有一定作用。干旱胁迫下草地早熟禾两个品种的脯氨酸含量升高,但多年生黑麦草品种脯氨酸含量变化不一致。乙烯利处理能够下调干旱胁迫下草地早熟禾脯氨酸脱氢酶基因(Proline Dehydrogenase, Pro DH)的表达,从而抑制脯氨酸降解。综上,通过抑制Pro DH基因表达,促进脯氨酸积累可能是乙烯利提高草地早熟禾的耐旱性的机制之一。, authors=唐雅楠1, 韩露1, 段梦琪1, 杜广啟1, 许立新1, 韩烈保1, authorsList=唐雅楠, 韩露, 段梦琪, 杜广啟, 许立新, 韩烈保, authorCompany=1 北京林业大学草业与草原学院, 北京 100083, correspAuthors=null, authorNote=唐雅楠(2001-),女,汉族,四川内江人,硕士研究生,主要从事草坪草抗逆生理研究,E-mail:15983264071@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, 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GAO Y N, HAN L B, XU L X. Effects of ethephon on the antioxidant enzyme genes expression of Poa pratensis under drought stress[J]. Acta Agrestia Sinica,2021,29(10):2200-2213 高娅楠,韩烈保,许立新. 乙烯利对干旱胁迫下草地早熟禾抗氧化酶基因表达的影响[J]. 草地学报,2021,29(10):2200-2213
ZHANG L, CHEN X, WU Y, et al. Research progress of proline in plant stress resistance[J]. Journal of Jianghan University Natural Science Edition,2023,51(1):42-51 张林,陈翔,吴宇,等. 脯氨酸在植物抗逆中的研究进展[J]. 江汉大学学报(自然科学版),2023,51(1):42-51
BOUDMYXAY K, SHEN L, ZHONG S, et al. Improving the antioxidant system and its stress resistance to tobacco seeds and seedling by proline priming[J]. Journal of Shanxi Agricultural Sciences,2019,47(1):39-48 BOUDMYXAY K,沈镭,钟帅,等. 脯氨酸引发提高烟草种子和幼苗抗逆性及其与抗氧化系统的关系[J]. 山西农业科学,2019,47(1):39-48
ZHAO X Q, ZHONG Y, ZHOU W Q, et al. Exogenous proline induced improvement of physiological response in maize (Zea mays) under low-temperature stress[J]. Molecular Plant Breeding,2023,21(5):1675-1684 赵小强,钟源,周文期,等. 外源脯氨酸诱导提高玉米对低温胁迫的生理响应[J]. 分子植物育种,2023,21(5):1675-1684
YE W X, ZHANG S N, LIU X T. Analysis on salt tolerance of 12 Poa pratensis varieties at seedling stage[J]. Acta Agrestia Sinica,2023,31(9):2740-2747 叶文兴,张胜男,刘雪婷. 12个草地早熟禾品种幼苗耐盐性分析[J]. 草地学报,2023,31(9):2740-2747
HUANG L, WANG L, LI C F, et al. Germplasm resources of genus Poa l.in Beijing area[J]. Acta Agrestia Sinica,2011,19(5):760-765 黄丽,王璐,李存福,等. 北京地区早熟禾属植物种质资源研究[J]. 草地学报,2011,19(5):760-765
CENZANO A M, VARELA M C, BERTILLER M B, et al. Effect of drought on morphological and functional traits of Poa ligularis and Pappostipa speciosa, native perennial grasses with wide distribution in Patagonian rangelands, Argentina[J]. Australian Journal of Botany,2013,61(5):383-393
MA Y, ZHANG J H, GAO Y N, et al. Effect of ethephon on chlorophyll metabolism genes expression of Kentucky bluegrass under drought stress[J]. Chinese Journal of Grassland,2022,44(12):1-10 马媛,张嘉航,高娅楠,等. 干旱胁迫下乙烯利对草地早熟禾叶绿素代谢基因表达的影响[J]. 中国草地学报,2022,44(12):1-10
XIONG X Y, GUO Q Q. Advances in fructan metabolism in perennial ryegrass and its role in stress tolerance[J]. Chinese Journal of Grassland,2025,47(8):120-131 熊心玥,郭倩倩. 多年生黑麦草果聚糖代谢及其参与植物抗逆作用的研究进展[J]. 中国草地学报,2025,47(8):120-131
GUAN H, YAO J M, ZHANG X Y, et al. Analysis of the transcription pattern and resistance function of LpPIL15-like gene in perennial ryegrass[J]. Chinese Journal of Grassland,2024,46(2):24-34 关昊,姚佳明,张欣栎,等. 多年生黑麦草LpPIL15-like基因转录模式及耐逆功能分析[J]. 中国草地学报,2024,46(2):24-34
DACOSTA M, HUANG B R. Changes in antioxidant enzyme activities and lipid peroxidation for bentgrass species in response to drought stress[J]. Journal of the American Society for Horticultural Science,2007,132(3):319-326
ZHANG X Z, WANG K H, ERVIN E H. Bermudagrass freezing tolerance associated with abscisic acid metabolism and dehydrin expression during cold acclimation[J]. Journal of the American Society for Horticultural Science,2008,133(4):542-550
ZHANG J H, GAO Y N, XU L X, et al. Transcriptome analysis of Kentucky bluegrass subject to drought and ethephon treatment[J]. PLoS One,2021,16(12):e0261472
ZHANG N, HAN L, XU L X, et al. Ethephon seed treatment impacts on drought tolerance of Kentucky bluegrass seedlings[J]. HortTechnology,2018,28(3):319-326
GUO Q, LIU Y, SUN N, et al. The role of proline in plant resistances to oxidative stress induced by abiotic stresses[J]. Journal of Plant Genetic Resources,2025,26(11):2085-2095 郭琪,刘悦,孙楠,等. 脯氨酸在植物抵御非生物胁迫诱导的氧化应激中的作用[J]. 植物遗传资源学报,2025,26(11):2085-2095
DUAN M Q, PENG C, ZHANG R, et al. Effects of ethephon on physiological indexes of perennial ryegrass under drought conditions[J]. Acta Agrestia Sinica,2021,29(2):333-341 段梦琪,彭春,张睿,等. 乙烯利对干旱条件下多年生黑麦草生理指标的影响[J]. 草地学报,2021,29(2):333-341
HAN L, HAN L B, XU L X. Effects of ethephon treatment on plant drought tolerance(review)[J]. Acta Agrestia Sinica,2013,21(4):631-636 韩露,韩烈保,许立新. 乙烯利影响植物抗旱性研究进展[J]. 草地学报,2013,21(4):631-636
HAN L. Effects of ethephon treatment on drought resistance of[D]. Beijing:Beijing Forestry University,2014:43 韩露. 乙烯利对草地早熟禾(Poa pratensis L.)抗旱性的影响[D]. 北京:北京林业大学,2014:43
XU X M, YE H C, LI G F. Progress in synthesis and metabolism of proline and its relationship with osmotolerance of plants[J]. Chinese Bulietin of Botany,2000,17(6):536-542 许祥明,叶和春,李国凤. 脯氨酸代谢与植物抗渗透胁迫的研究进展[J]. 植物学通报,2000,17(6):536-542)
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草地学报 |研究论文 2026 , 34 (9) : 3284 -3291
乙烯利对草坪草干旱胁迫下脯氨酸等生理指标的影响
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唐雅楠1, 韩露1, 段梦琪1, 杜广啟1, 许立新1, 韩烈保1
作者信息
    1 北京林业大学草业与草原学院, 北京 100083
作者简介:
唐雅楠(2001-),女,汉族,四川内江人,硕士研究生,主要从事草坪草抗逆生理研究,E-mail:15983264071@163.com
Effects of Ethephon on Physiological Indicators such as Proline in Turfgrass under Drought Stress
  • TANG Ya-nan1, HAN Lu1, DUAN Meng-qi1, DU Guang-qi1, XU Li-xin1, HAN Lie-bao1
  • Affiliations
      1 School of Grassland Science of Beijing Forestry University, Beijing 100083, China
    doi: 10.11733/j.issn.1007-0435.2026.09.013
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    适宜浓度的乙烯利可以提升草坪草的耐旱性,但具体作用机制未知。脯氨酸作为渗透调节物质,在植物逆境胁迫响应中发挥重要作用。本文以草地早熟禾(Poa pratensis)和多年生黑麦草(Lolium perenne)为材料,检测乙烯利对干旱胁迫下草坪草生理指标的影响,并分析脯氨酸合成与降解代谢相关基因表达的变化,探讨脯氨酸在乙烯利提升草坪草抗旱性中的作用。结果表明:乙烯利可缓解干旱胁迫诱导的草坪草叶片相对含水量的下降和电解质渗透率的上升,对耐旱性提升有一定作用。干旱胁迫下草地早熟禾两个品种的脯氨酸含量升高,但多年生黑麦草品种脯氨酸含量变化不一致。乙烯利处理能够下调干旱胁迫下草地早熟禾脯氨酸脱氢酶基因(Proline Dehydrogenase, Pro DH)的表达,从而抑制脯氨酸降解。综上,通过抑制Pro DH基因表达,促进脯氨酸积累可能是乙烯利提高草地早熟禾的耐旱性的机制之一。
    乙烯利  /  草坪草  /  脯氨酸  /  干旱胁迫  /  基因表达
    Appropriate concentrations of ethephon can improve the drought tolerance of turfgrasses, but the specific mechanism of action remains unclear. As an osmotic regulator, proline plays an important role in plant responses to stress. In this study, Kentucky bluegrass (Poa pratensis) and perennial ryegrass (Lolium perenne) were used as materials. We examined the effects of ethephon on physiological indices of turfgrasses in response to drought stress, analyzed changes in the expression of genes related to proline biosynthesis and catabolism, and explored the role of proline in ethephon-improved drought resistance of turfgrasses. The results showed that ethephon alleviated the decrease of relative water content and the increase of electrolyte leakage of turfgrass leaves induced by drought stress, thus contributing to improved drought tolerance. Under drought stress, proline content increased in the two Poa pratensis cultivars, whereas the changes were inconsistent among Lolium perenne cultivars. Ethephon treatment down-regulated the expression of Proline Dehydrogenase (Pro DH) gene in Poa pratensis under drought stress, thereby inhibiting proline degradation. In conclusion, promoting proline accumulation by suppressing the expression of the Pro DH gene might be one of the mechanisms by which ethephon improves drought tolerance in Poa pratensis.
    Ethephon  /  Turfgrass  /  Proline  /  Drought stress  /  Gene expression
    唐雅楠, 韩露, 段梦琪, 杜广啟, 许立新, 韩烈保. 乙烯利对草坪草干旱胁迫下脯氨酸等生理指标的影响. 草地学报, 2026 , 34 (9) : 3284 -3291 . DOI: 10.11733/j.issn.1007-0435.2026.09.013
    TANG Ya-nan, HAN Lu, DUAN Meng-qi, DU Guang-qi, XU Li-xin, HAN Lie-bao. Effects of Ethephon on Physiological Indicators such as Proline in Turfgrass under Drought Stress[J]. Acta Agrestia Sinica, 2026 , 34 (9) : 3284 -3291 . DOI: 10.11733/j.issn.1007-0435.2026.09.013

      北京林业大学“5·5工程”科研创新团队项目 (BLRC2023A04)

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    GAO Y N, HAN L B, XU L X. Effects of ethephon on the antioxidant enzyme genes expression of Poa pratensis under drought stress[J]. Acta Agrestia Sinica,2021,29(10):2200-2213 高娅楠,韩烈保,许立新. 乙烯利对干旱胁迫下草地早熟禾抗氧化酶基因表达的影响[J]. 草地学报,2021,29(10):2200-2213
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    MA Y, ZHANG J H, GAO Y N, et al. Effect of ethephon on chlorophyll metabolism genes expression of Kentucky bluegrass under drought stress[J]. Chinese Journal of Grassland,2022,44(12):1-10 马媛,张嘉航,高娅楠,等. 干旱胁迫下乙烯利对草地早熟禾叶绿素代谢基因表达的影响[J]. 中国草地学报,2022,44(12):1-10
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