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2 Academy of Animal and Veterinary Science, Qinghai University, Xining, Qinghai Province 810016, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574995068678752, articleId=1309574994636665439, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=饲用燕麦高效EMS诱变技术优化与高产种质筛选, columnId=1309565376569114713, journalTitle=草地学报, columnName=技术研发, runingTitle=null, highlight=null, articleAbstract=本研究以‘中天4号’饲用燕麦（Avena sativa L.）种子为材料，通过去除稃皮和连续30 h种子含水量调节的前处理，不同浓度和时间的EMS诱变处理，以及赤霉素外源施加的后处理，优化饲用燕麦EMS诱变技术体系。构建M₂代突变群体，于乳熟期调查其农艺性状，评估诱变效率并筛选高产种质。结果表明：98%浓硫酸浸泡6 min可有效去除饲用燕麦稃皮障碍，调节种子含水量至50%（4 h浸泡）可促进EMS高效地进入种子，筛选出饲用燕麦EMS最佳诱变条件为1.0% EMS处理16 h，采用50 mg·L⁻¹外源赤霉素处理诱变种子可显著促进诱变后幼苗生长（P<0.05），与未添加赤霉素的对照相比，发芽率显著提高8.5%，苗长也恢复至未诱变幼苗水平。该EMS诱变技术系统性地克服了饲用燕麦种子渗透障碍、生理状态不一及诱变损伤等关键限制因素。M₂代获得高产潜力种质的诱变效率达到1.92%，筛选到13份表现优异的高产潜力种质。, authors=钟志花¹, 卢茂金¹, 王蓉¹, 杨国栋¹, 刘朝¹, 马祥², 闫明科¹, 王亚芳¹, 郭欢¹, authorsList=钟志花, 卢茂金, 王蓉, 杨国栋, 刘朝, 马祥, 闫明科, 王亚芳, 郭欢, authorCompany=1 西北农林科技大学草业与草原学院, 陕西 杨凌 712100;
2 青海大学畜牧兽医科学院, 青海 西宁 810016, correspAuthors=null, authorNote=钟志花(2001-),女,汉族,青海海东人,硕士研究生,主要从事牧草种质资源与遗传育种研究,E-mail:zhihua@nwafu.edu.cn, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=Usr0ZZJDPOecBF95/jCQpg==, pdfFileSize=1744630, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null)}, authors=[Author(id=1320576441952096812, tenantId=1146029695717560320, journalId=null, articleId=1309574994636665439, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={EN=AuthorExt(id=null, tenantId=null, journalId=1308397196074385509, 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