1 College of Landscape Architecture & Arts, Northwest A&F University, Yangling, Shaanxi 712100, China; 2 Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, Xinjiang 830000, China
doi: 10.11733/j.issn.1007-0435.2026.08.024
文章导航
摘要
收起
为探讨槲皮素对盐胁迫下罗布红麻(Apocynum venetum L.)种子萌发和幼苗生长的调控机制,明确其适宜施用浓度。本研究以罗布红麻为材料,设置8个处理(HCK:正常对照;HCKN:200 mmol·L⁻¹NaCl盐胁迫;Q₁+N~Q₆+N:200 mmol·L⁻¹NaCl+50,100,150,200,250,300 μmol·L⁻¹槲皮素),系统测定种子发芽特性(发芽率、发芽势、发芽指数)、幼苗生长指标(株高、根系生长参数、生物量、叶片生长参数)、光合生理特性(光合色素含量、叶绿素荧光参数)及抗逆生理指标(抗氧化酶活性、丙二醛含量、脯氨酸含量),并通过隶属函数法综合评价缓解效应。结果表明:盐胁迫对罗布红麻种子发芽率影响较小,但显著降低发芽势与发芽指数,并严重抑制罗布红麻幼苗生长及光合系统功能。施用槲皮素后,发现其对盐胁迫的缓解效应呈现显著浓度依赖性:低浓度槲皮素(Q₁+N,Q₂+N)缓解作用有限,高浓度槲皮素(Q₅+N,Q₆+N)则无法有效改善甚至抑制罗布红麻幼苗生长。相比于其他斛皮素浓度处理组,150 μmol·L⁻¹槲皮素(Q₃+N)处理缓解盐胁迫效果最佳:较盐胁迫组(HCKN),其种子发芽势和发芽指数分别提高2.1%和3.5%,幼苗株高提高97.2%,叶绿素总含量和类胡萝卜素含量分别提高17.1%和34.9%,脯氨酸含量提高20.6%,丙二醛含量降低22.8%(P<0.05),同时显著改善了根系形态与叶绿素荧光参数,其中根长、根表面积较HCKN组分别提升37.2%,51.3%;光系统Ⅱ最大光化学效率(Maximum quantum efficiency of photo-system II,Fv/Fm)、光系统Ⅱ实际光化学效率(Effective quantum yield of photosystem II,Y(II))均得到明显改善,有效缓解了光系统 Ⅱ(Photosystem II,PSII)反应中心损伤并且提高了实际光合效率,使幼苗生长及生理特性接近正常对照水平。研究表明,槲皮素可通过促进光合色素合成、增强抗氧化系统功能、调节渗透平衡及优化根系结构,协同提升罗布红麻幼苗耐盐性,有效缓解盐胁迫损伤。该研究明确了槲皮素调控罗布红麻耐盐性的适宜浓度与关键生理机制,为盐碱地罗布红麻规模化栽培及生态修复提供了重要技术参考。
关键词
槲皮素
/
罗布红麻
/
盐胁迫
/
种子萌发
/
幼苗生长
Abstract
收起
This study investigated the growth regulation mechanism of quercetin on seed germination and seedling growth of Apocynum venetum L. under salt stress, and determined its optimal application concentration. This study utilized A. venetum as the experimental material, and established eight treatments: HCKN: 200 mmol·L-1 NaCl salt stress; Q1+N-Q6+N: 200 mmol·L-1 NaCl +50, 100, 150, 200, 250, 300 μmol·L-1 quercetin). The study systematically measured seed germination traits (germination rate, germination vigor, germination index), seedling growth indicators (plant height, root growth parameters, biomass, leaf growth parameters), photosynthetic physiological traits (photosynthetic pigment content, chlorophyll fluorescence parameters), and stress resistance physiological indicators (antioxidant enzyme activity, malondialdehyde content, proline content). The alleviation effects were comprehensively evaluated using the membership function method. The results indicated that salt stress had a minor effect on the germination rate of A. venetum seeds but significantly reduced germination vigor and germination index. It severely inhibited the growth of A. venetum seedlings and impaired photosynthetic system function, characterized by significantly decreasing photosynthetic pigment content and PSII photochemical efficiency, along with increasing accumulation of malondialdehyde, a lipid peroxidation product.The salt stress-alleviating effect of quercetin exhibited a pronounced concentration-dependent response: Low quercetin concentrations (Q1+N, Q2+N) provided limited alleviation, while high concentrations (Q5+N, Q6+N) failed to effectively improve growth and even inhibited A. venetum seedling development. Compared to other quercetin concentration treatments, the 150 μmol·L-1 quercetin (Q3+N) treatment showed the best salt stress alleviation effect. Compared to the salt-stressed control (HCKN), it increased seed germination potential and germination index by 2.1% and 3.5%, respectively. Seedling height increased by 97.2%, total chlorophyll content and carotenoid content increased by 17.1% and34.9% respectively, proline content increased by 20.6%, and malondialdehyde content decreased by 22.8% (P<0.05). Concurrently, it significantly improved root morphology and chlorophyll fluorescence parameters, bringing seedling growth and physiological characteristics closer to the normal control level. Quercetin enhances salt tolerance in A. venetum seedlings by synergistically promoting photosynthetic pigment synthesis, strengthening antioxidant system function, regulating osmotic balance, and optimizing root structure, thereby effectively mitigating salt stress damage. This study identifies the optimal concentration and key physiological mechanisms by which quercetin regulates A. venetum’s salt tolerance, providing crucial technical guidance for large-scale cultivation and ecological restoration of A. venetum in saline-alkali soils.
Key words
Quercetin
/
Apocynum venetum L.
/
Salt stress
/
Seed germination
/
Seedling growth
ZHAO Xue-qing, JIANG Li, ZHAO Bing, CHEN Yu-qian.
Effects of Different Concentrations of Quercetin on Seed Germination and Seedling Growth of Apocynum venetum L. under Salt Stress[J].
Acta Agrestia Sinica,
2026
, 34
(8)
: 3015
-3025
.
DOI: 10.11733/j.issn.1007-0435.2026.08.024
基金
收起
新疆自治区重点研发项目 (2023B02045)
参考文献
收起
文献
收起
参考文献引证文献
排序方式:
VAN Z E,ZHANG Y X,TESTERINK C. Salt tolerance mechanisms of plants[J]. Annual Review of Plant Biology,2020,71:403-433 国家发展改革委,自然资源. 国家发展改革委自然资源部关于印发《全国重要生态系统保护和修复重大工程总体规划(2021-2035年)》的通知(发改农经〔2020〕837号[EB/OL].https://www.gov.cn/zhengce/zhengceku/2020-06/12/content_5518982.htm, 2020-06-03 LI R Y,CHAI W W,GUO X N,et al. Effect of salt treatment on osmotic adjustment of Apocynum venetum seedlings[J]. Molecular Plant Breeding,2020,18(12):4105-4110李茹玉,柴薇薇,郭晓农,等. 盐处理对罗布麻(Apocynum venetum)幼苗渗透调节的影响[J]. 分子植物育种,2020,18(12):4105-4110 LI L L,WANG J Y,QIAN C, et al. Physiological and molecular responses of Apocynum venetum L. (Apocynaceae) on salt stress[J]. Horticulturae,2023,9(9):1010 舒开林,赵亚楠,王建鑫,等. 盐胁迫下对植物生长影响的研究进展[C]// 华北五省市(区)环境科学学会第二十二届学术年会论文集.大同:2021:1-7 WANG P S,JIA L,ZHANG J L,et al. Comparative study on salt tolerance of Apocynum venetum under different salt stresses[J]. Chinese Agricultural Science Bulletin,2021,37(12):39-44王鹏山,贾林,张金龙,等. 不同盐胁迫下罗布麻种子耐盐性的比较研究[J]. 中国农学通报,2021,37(12):39-44 WANG S,LI J,WU Y J,et al. Effects of salt stress on the growth and physiology of Apocynum venetum[J]. Pratacultural Science,2022,39(9):1832-1841王珊,李静,吴玉洁,等. 盐胁迫对罗布麻生长和生理的影响[J]. 草业科学,2022,39(9):1832-1841 CHEN H,XU L X,WANG M Q,et al. Effects of exogenous gibberellin and melatonin on seed germination, seedling growth and physiology of Trifolium repens under three stresses[J]. Acta Agrestia Sinica,2024,32(3):781-792陈红,许凌欣,王梦琦,等. 外源赤霉素和褪黑素对三种胁迫下白三叶种子萌发、幼苗生长及生理的影响[J]. 草地学报,2024,32(3):781-792 ZHU F F,LU C S,ZHANG Y F,et al. Effects of spraying exogenous salicylic acid on growth of rice seedlings under low temperature stress[J]. Chinese Agricultural Science Bulletin,2025,41(33):6-13朱菲菲,陆楚盛,张玉芬,等. 外源水杨酸喷施对低温胁迫下水稻幼苗生长的影响[J]. 中国农学通报,2025,41(33):6-13 DONG X X,MIU B L,WANG Y N,et al. Effects of exogenous betaine on strawberry growth under saline-alkali stress[J]. Jiangsu Agricultural Sciences,2025,53(13):197-203董向向,缪百灵,王燕娜,等. 外源甜菜碱对盐碱胁迫下草莓生长的影响[J]. 江苏农业科学,2025,53(13):197-203 JANCZAK-PIENIAZEK M.,MIGUT D.,PIECHOWIAK T. Enhancement of photosynthetic efficiency and antioxidant response in wheat under drought stress by quercetin-copper complex[J]. International Journal of Molecular Sciences,2025,26(21):10365 LIU G C,ZHANG Z,TIAN Y,et al. VvbZIP22 regulates quercetin synthesis to enhance cold resistance in grape[J]. Plant Science,2025,350:112293 HAN L,WU X H,GUO J J, et al. Exogenous quercetin treatment provides insights into transcriptional regulatory network for salt tolerance of Triticum urartu[J]. Physiologia Plantarum,2025,177(3):e70310 YANG J L. Preliminary study on antioxidation of quercetin promoting seed germination and seedling growth of Apocynum venetum under drought[D]. Harbin: Heilongjiang University,2021:1-75杨佳乐. 槲皮素促进干旱下罗布麻种子萌发和幼苗生长的抗氧化初步研究[D]. 哈尔滨:黑龙江大学,2021:1-75 JI Y C,ZHAO X Q,ZHAO B,et al. Effects of quercetin and kaempferol on seed germination and seedling growth in Apocynum pictum Schrenk under salt stress[J]. Arid Zone Research,2025,42(8):1425-1432季雨辰,赵雪情,赵冰,等. 槲皮素和山奈酚对盐胁迫下白麻种子萌发和幼苗生长的影响[J]. 干旱区研究,2025,42(8):1425-1432 SHI Q M,DENG F Y,WU M Y,et al. Study on salt tolerance of Apocynum venetum Linn. and Poacynum hendersonii (Hook. f.) Woodson at stages of seed germination and seedlings growth[J]. Northern Horticulture,2014(12):128-133石秋梅,邓翻云,吴敏言,等. 罗布麻和大叶白麻种子萌发及幼苗生长耐盐性研究[J]. 北方园艺,2014(12):128-133 JANCZAK-PIENIAZEK M.,MIGUT D.,PIECHOWIAK, T, et al. Assessment of the impact of the application of a quercetin-copper complex on the course of physiological and biochemical processes in wheat plants(Triticum aestivum L.)growing under saline conditions[J]. Cells,2022,11(7):1141 TONG Y H,WANG X T,MA Y L,et al. Effects of a chitosan seed soaking treatment on seed germination and growth of alfalfa under saline alkali stress[J]. Acta Prataculturae Sinica,2026,35(3):245-256童玉花,王晓彤,马永龙,等. 壳聚糖浸种对盐碱胁迫下紫花苜蓿种子萌发的影响[J]. 草业学报,2026,35(3):245-256 HAN Z J,WANG H Z. Guidance for plant physiology experiments[M]. Beijing: China Agricultural Science and Technology Press,2002:98-107韩占江,王海珍. 植物生理学实验指导[M]. 北京:中国农业科学技术出版社,2023:98-107 WANG S Y,ZHANG G,YANG S C,et al. Comprehensive evaluation on substrates for Phalaenopsis culture by membership function method[J]. Northern Horticulture,2025(08):72-81王世尧,张果,杨书才,等. 基于隶属函数法的蝴蝶兰栽培基质综合评价[J]. 北方园艺,2025(8):72-81 FANG J W,WU Y,LIU Z H. Effects of salt stress on seed germination and physiological characteristics of Apocynum venetum[J]. Crops,2018(4):167-174方婧雯,邬燕,刘志华. 盐胁迫对罗布麻种子萌发及生理特性的影响[J]. 作物杂志,2018(4):167-174 ZHANG Y,XIA Y X. Quercetin enhances tomato seed germination via phenylpropanoid-dependent regulation of ROS,hormone signaling,and starch hydrolysis[J]. Plant Physiology and Biochemistry,2026,230:110590 BAIS H P,VEPACHEDU R,GILROY S,et al. Allelopathy and exotic plant invasion:From molecules and genes to species interactions[J]. Science,2003,301(5638):1377-1380 LIU C Y,JIANG X B,YUAN Z H. Plant responses and adaptations to salt stress: a review[J]. Horticulturae,2024,10(11):1221 PARVIN K,HASAUZZAMAN M,BHUYAN MHMB,et al.Quercetin mediated salt tolerance in tomato through the enhancement of plant antioxidant defense and glyoxalase systems[J]. Plants,2019,8(8):247 MALONEY G S,DINAPOLI K T,MUDAY G K. The anthocyanin reduced tomato mutant demonstrates the role of flavonols in tomato lateral root and root hair development[J]. Plant Physiology,2014,166(2):614-631 LIU C X,LIU L Y,LIU X L, et al. Salt stress inhibits photosynthesis and destroys chloroplast structure by downregulating chloroplast development-related genes in Robinia pseudoacacia seedlings[J]. Plants,2023,12(6):1283 WANG B Q,ZHAO Y,ZHU X L,et al. Effects of saline-alkali stress on photosynthesis characteristics and antioxidant system in quinoa seedlings leaf[J]. Acta Agrestia Sinica,2021,29(8):1832-1841王宝强,赵颖,朱晓林,等. 盐碱胁迫对藜麦幼苗叶片光合特性及抗氧化系统的影响[J]. 草地学报,2021,29(8):1690-1696 PROCHÁZKOVÁ D,BOUSOVÁ I,WILHELOVÁ N. Antioxidant and prooxidant properties of flavonoids[J]. Fitoterapia,2011,82(4):513-523 ZHAO Q H,ZHENG X L,WANG C, et al. Exogenous melatonin improves drought tolerance by regulating the antioxidant defense system and photosynthetic efficiency in fodder soybean seedings[J]. Plants,2025,14(3): 460 LI F,ZHANG J,XIE J M,et al. Effects of exogenous salicylic acid on growth and physiological characteristics of lettuce seedlings under salt stress[J]. Journal of Gansu Agricultural University,2024,59(6):160-168李凤,张婧,颉建明,等. 外源水杨酸对盐胁迫下莴笋幼苗生长及生理特性的影响[J]. 甘肃农业大学学报,2024,59(6):160-168 SUN Y J,QIN D L,YI F,et al. Effects of salicylic acid on growth and physiological property of maize seedling under low temperature stress[J]. Jiangsu Journal of Agricultural Sciences,2018,34(4):726-734孙玉珺,秦东玲,伊凡,等. 外源水杨酸对低温胁迫下玉米幼苗生长及生理特性的影响[J]. 江苏农业学报,2018,34(4):726-734