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SSR molecular markers were employed to authenticate true hybrids, followed by analyses of diversity, correlation, clustering, and principal components for 10 yield-related traits in the true hybrid population. The results revealed that 300 true hybrids were identified using two primer pairs (SSR24 and WSSR31). The true hybrid population exhibited abundant phenotypic variation, with coefficients of variation ranging from 13.91% to 45.58% and diversity indices from 1.37 to 2.84. Correlation analysis demonstrated that all traits were highly significantly positively correlated with single-plant yield (P<0.01), among which plant height showed the strongest correlations with fresh weight and dry weight, with correlation coefficients of 0.55 and 0.66, respectively. Heterosis analysis indicated that most yield-related traits of the F1 generation displayed positive dominance, and the relative heritability of the female parent was stronger than that of the male parent. Principal component analysis extracted two principal components, accounting for a cumulative variance contribution rate of 64.819%, with plant height, single-plant dry weight, tiller number, and internode length identified as core influencing factors., authors=HE Chao-xin1, YU Shuai1, ZHANG Xin-quan1, PENG Yan1, authorsList=HE Chao-xin, YU Shuai, ZHANG Xin-quan, PENG Yan, authorCompany=1 College of Grassland Science and Technology of Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575006095508066, articleId=1309575002182222433, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=象草F₁代真假杂种鉴定及高产种质的综合评价与筛选, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为筛选出高产潜力的象草(Pennisetum purpureum)新种源,以‘桂牧1号’杂交象草为母本、F-AI象草为父本获得800份杂交F₁代,用SSR分子标记鉴定真假杂种,并对真杂种的10个产量相关性状开展多样性、相关性、聚类和主成分分析。结果表明:用SSR24和WSSR31两对引物鉴定出300份真杂种。真杂种群体表型变异丰富,变异系数为13.91%~45.58%,多样性指数为1.37~2.84。相关性分析显示,所有性状均与单株产量呈极显著正相关(P<0.01),其中株高与鲜重、干重的相关系数最高,为0.55和0.66。杂种优势分析表明,F₁代的产量及相关性状多为正向显性,母本的相对遗传力强于父本。主成分分析提取出2个主成分,累计贡献率为64.819%,株高、单株干重、分蘖数和节间长为核心影响因子。聚类分析将材料划分为4类,其中,第Ⅲ类群有92份材料综合性状最优,第Ⅱ类群有41份材料综合性状表现最差,第I、Ⅳ类群介于两者之间。通过模糊隶属函数法进行综合评价,筛选出15份具有高产潜力的优良育种材料。研究结果为象草杂交育种提供了方法和材料支撑。, authors=贺朝鑫1, 余帅1, 张新全1, 彭燕1, authorsList=贺朝鑫, 余帅, 张新全, 彭燕, authorCompany=1 四川农业大学草业科技学院, 四川 成都 611130, correspAuthors=null, authorNote=贺朝鑫(2001-),男,汉族,四川宜宾人,硕士研究生,主要从事草种质资源创新及育种研究,E-mail:1970727216@qq.com, correspAuthorsNote=null, copyrightStatement=null, 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SU C C,JIN Y,XU F,et al. Identification of F1 hybrids of Vitis davidii Foex using SSR molecular markers[J]. Jiangsu Agricultural Sciences,2018,46(17):35-38苏聪聪,金燕,徐丰,等. 利用SSR分子标记鉴定刺葡萄F1代杂种[J]. 江苏农业科学,2018,46(17):35-38
DO AMARAL SANTOS DE CARVALHO ROCHA J R,MACHADO J C,CARNEIRO P C S,et al. Bioenergetic potential and genetic diversity of elephantgrass via morpho-agronomic and biomass quality traits[J]. Industrial Crops and Products,2017,95:485-492
LIU W Y,LIANG Y C,LIU J,et al. Comparative experiment on field cultivation of different varieties of Pennisetum[J]. Guangxi Journal of Animal Husbandry & Veterinary Medicine,2017,33(4):173-175刘伟燕,梁源春,刘婕,等. 不同品种狼尾草的田间栽培比较试验[J]. 广西畜牧兽医,2017,33(4):173-175
Ministry of Agriculture of the People’s Republic of China. Guidelines for the conduct of tests for distinctness, uniformity and stability-Pennisetum: NY/T 2356-2013. Beijing: China Agriculture Press,2013中华人民共和国农业部. 植物新品种特异性、一致性和稳定性测试指南-狼尾草属: NY/T 2356-2013. 北京:中国农业出版社. 2013
WU B C,ZHANG H,TONG L,et al. Research on genetic and phenotypic differences of Pennisetum purpureum mutants generated by irradiation[J]. Acta Prataculturae Sinica,2018,27(11):77-86武炳超,张欢,童磊,等. 象草不同辐射剂量诱变系表型及遗传变异研究[J]. 草业学报,2018,27(11):77-86
LI Q Y,LIU T,ZHANG S H,et al. Development and application of survey sequencing and gSSR molecular marker of Pennisetum glaucum[J]. Molecular Plant Breeding,2019,17(16):5375-5382李青遥,刘婷,张曙恒,等. 美洲狼尾草Survey测序及其gSSR分子标记的开发应用[J]. 分子植物育种,2019,17(16):5375-5382
WANG C R.Development and Application of G-SSR Molecular Markers Based on Genome Survey Sequencing of Pennisetum purpureum[D]. Chengdu: Sichuan Agricultural University, 2019:9王成然. 基于象草Survey测序的G-SSR分子标记开发与应用[D].成都:四川农业大学,2019:9
CHANG L F,LI X,GUO H J,et al. Genetic diversity analysis and comprehensive evaluation of octoploid Tritipyrumderived wheat breeding lines based on agronomic traits[J]. Acta Prataculturae Sinica,2022,31(11):61-74常利芳,李欣,郭慧娟,等. 小偃麦衍生系表型遗传多样性分析及综合评价[J]. 草业学报,2022,31(11):61-74
PEI X S. A comparative analysis between the path coefficient and relative hereditability[J]. Hereditas,1982,4(2):28-30裴新澍. 通径系数与相对遗传力的比较分析[J]. 遗传,1982,4(2):28-30
UPADHYAYA H D,SHARMA S,RAMULU B,et al. Variation for qualitative and quantitative traits and identification of trait-specific sources in new sorghum germplasm[J]. Crop & Pasture Science,2010,61(8):609-618
LIU D L,WANG X Q,LI W S,et al. Genetic diversity analysis of the phenotypic traits of 215 sugar beet germplasm resources[J]. Sugar Tech,2022,24(6):1790-1800
GUO Y D,LIU L Y,YUE Y S,et al. Development of SSR markers based on transcriptome sequencing and verification of their conservation across species of ornamental Pennisetum rich. (Poaceae)[J]. Agronomy,2022,12(7):1683-1683
DU H Q,FENG C S,LÜ X Z,et al. Evaluation and analysis of grass yield and related agronomic traits of 87 alfalfa varieties[J].Acta Ecologae Animalis Domastici,2025,46(5):90-99杜红旗,冯长松,吕先召,等. 87个苜蓿品种草产量及其相关农艺性状评价分析[J]. 家畜生态学报,2025,46(5):90-99
GUO J,WEI L,YANG Y P,et al. Analysis of heterosis and phenotypic variation for F1 progeny of interspecific hybridization of rye[J]. Jiangsu Agricultural Sciences,2022,50(1):86-90郭娟,魏莱,杨燕萍,等. 黑麦种间杂交F1代杂种优势及表型变异分析[J]. 江苏农业科学,2022,50(1):86-90
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YANG J. Selection of parents of Medicago hybridization and analysis of growth superiority in F1 seedling stage[D]. Hohhot:Inner Mongolia Agricultural University,2021:44-37杨婕. 苜蓿杂交亲本选择及其杂种F1苗期生长优势分析[D]. 呼和浩特:内蒙古农业大学,2021:44-37
JIA X Y. Physiological evaluation of drought resistance and DNA methylation of F1 interspecific hybrids[D]. Hohhot:Inner Mongolia Agricultural University,2022:11-34贾学宇. 披碱草属种间杂种F1抗旱性生理评价及DNA甲基化研究[D]. 呼和浩特:内蒙古农业大学,2022:11-34
LIU J W,HAO X Y,DU Y Q,et al. Studies on phenotypic genetic variation of F1 hybrids of Trifolium ambiguum bieb. and T. Repens L[J]. Chinese Journal of Grassland,2024,46(8):37-44刘嘉伟,郝新艳,杜雨芊,等. 高加索三叶草与白三叶杂种F1代表型遗传变异研究[J]. 中国草地学报,2024,46(8):37-44
BAI Y L,LI Z M,ZHAO J P,et al. Comparative experiment of alfalfa varieties[J]. Inner Mongolia Prataculture,2010,22(1):55-59白玉龙,李志明,赵建平,等. 紫花苜蓿品种比较试验[J]. 内蒙古草业,2010,22(1):55-59
LIANG G H L,RIEDL W A. Agronomic traits influencing forage and seed yield in alfalfa[J]. Crop Science,1964,4(4):394-396
LLOVERAS J,FERRAN J,ALVAREZ A,et al. Harvest management effects on alfalfa (Medicago sativaL.) production and quality in Mediterranean areas[J]. Grass and Forage Science,1998,53(1):88-92
SENGUL S. Yield components,morphology and forage quality of native alfalfa ecotypes[J]. Journal of Biological Sciences,2002,2(7):494-498)
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象草F₁代真假杂种鉴定及高产种质的综合评价与筛选
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贺朝鑫, 余帅, 张新全, 彭燕
草地学报 | 研究论文 2026,34(8): 2980-2990
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草地学报 |研究论文 2026 , 34 (8) : 2980 -2990
象草F₁代真假杂种鉴定及高产种质的综合评价与筛选
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贺朝鑫, 余帅, 张新全, 彭燕
作者信息
作者简介:
贺朝鑫(2001-),男,汉族,四川宜宾人,硕士研究生,主要从事草种质资源创新及育种研究,E-mail:1970727216@qq.com
Identification of True Hybrids in the F1 Generation of Elephant Grass and Comprehensive Evaluation and Screening of High-yield Germplasm
HE Chao-xin, YU Shuai, ZHANG Xin-quan, PENG Yan
Affiliations
    1 College of Grassland Science and Technology of Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China
doi: 10.11733/j.issn.1007-0435.2026.08.021
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为筛选出高产潜力的象草(Pennisetum purpureum)新种源,以‘桂牧1号’杂交象草为母本、F-AI象草为父本获得800份杂交F₁代,用SSR分子标记鉴定真假杂种,并对真杂种的10个产量相关性状开展多样性、相关性、聚类和主成分分析。结果表明:用SSR24和WSSR31两对引物鉴定出300份真杂种。真杂种群体表型变异丰富,变异系数为13.91%~45.58%,多样性指数为1.37~2.84。相关性分析显示,所有性状均与单株产量呈极显著正相关(P<0.01),其中株高与鲜重、干重的相关系数最高,为0.55和0.66。杂种优势分析表明,F₁代的产量及相关性状多为正向显性,母本的相对遗传力强于父本。主成分分析提取出2个主成分,累计贡献率为64.819%,株高、单株干重、分蘖数和节间长为核心影响因子。聚类分析将材料划分为4类,其中,第Ⅲ类群有92份材料综合性状最优,第Ⅱ类群有41份材料综合性状表现最差,第I、Ⅳ类群介于两者之间。通过模糊隶属函数法进行综合评价,筛选出15份具有高产潜力的优良育种材料。研究结果为象草杂交育种提供了方法和材料支撑。
象草杂交F1代  /  分子标记  /  多样性分析  /  聚类分析  /  综合评价
To screen novel germplasm of Pennisetum purpureum with high-yield potential, 800 F1 hybrid individuals were generated by crossing ‘Guimu 1’ hybrid P. purpureum (female parent) with F-AI P. purpureum (male parent). SSR molecular markers were employed to authenticate true hybrids, followed by analyses of diversity, correlation, clustering, and principal components for 10 yield-related traits in the true hybrid population. The results revealed that 300 true hybrids were identified using two primer pairs (SSR24 and WSSR31). The true hybrid population exhibited abundant phenotypic variation, with coefficients of variation ranging from 13.91% to 45.58% and diversity indices from 1.37 to 2.84. Correlation analysis demonstrated that all traits were highly significantly positively correlated with single-plant yield (P<0.01), among which plant height showed the strongest correlations with fresh weight and dry weight, with correlation coefficients of 0.55 and 0.66, respectively. Heterosis analysis indicated that most yield-related traits of the F1 generation displayed positive dominance, and the relative heritability of the female parent was stronger than that of the male parent. Principal component analysis extracted two principal components, accounting for a cumulative variance contribution rate of 64.819%, with plant height, single-plant dry weight, tiller number, and internode length identified as core influencing factors.
Pennisetum purpureum hybrid F1 generation  /  SSR markers  /  Phenotypic diversity  /  Cluster analysis  /  Comprehensive evaluation
贺朝鑫, 余帅, 张新全, 彭燕. 象草F₁代真假杂种鉴定及高产种质的综合评价与筛选. 草地学报, 2026 , 34 (8) : 2980 -2990 . DOI: 10.11733/j.issn.1007-0435.2026.08.021
HE Chao-xin, YU Shuai, ZHANG Xin-quan, PENG Yan. Identification of True Hybrids in the F1 Generation of Elephant Grass and Comprehensive Evaluation and Screening of High-yield Germplasm[J]. Acta Agrestia Sinica, 2026 , 34 (8) : 2980 -2990 . DOI: 10.11733/j.issn.1007-0435.2026.08.021

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DO AMARAL SANTOS DE CARVALHO ROCHA J R,MACHADO J C,CARNEIRO P C S,et al. Bioenergetic potential and genetic diversity of elephantgrass via morpho-agronomic and biomass quality traits[J]. Industrial Crops and Products,2017,95:485-492
LIU W Y,LIANG Y C,LIU J,et al. Comparative experiment on field cultivation of different varieties of Pennisetum[J]. Guangxi Journal of Animal Husbandry & Veterinary Medicine,2017,33(4):173-175刘伟燕,梁源春,刘婕,等. 不同品种狼尾草的田间栽培比较试验[J]. 广西畜牧兽医,2017,33(4):173-175
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WU B C,ZHANG H,TONG L,et al. Research on genetic and phenotypic differences of Pennisetum purpureum mutants generated by irradiation[J]. Acta Prataculturae Sinica,2018,27(11):77-86武炳超,张欢,童磊,等. 象草不同辐射剂量诱变系表型及遗传变异研究[J]. 草业学报,2018,27(11):77-86
LI Q Y,LIU T,ZHANG S H,et al. Development and application of survey sequencing and gSSR molecular marker of Pennisetum glaucum[J]. Molecular Plant Breeding,2019,17(16):5375-5382李青遥,刘婷,张曙恒,等. 美洲狼尾草Survey测序及其gSSR分子标记的开发应用[J]. 分子植物育种,2019,17(16):5375-5382
WANG C R.Development and Application of G-SSR Molecular Markers Based on Genome Survey Sequencing of Pennisetum purpureum[D]. Chengdu: Sichuan Agricultural University, 2019:9王成然. 基于象草Survey测序的G-SSR分子标记开发与应用[D].成都:四川农业大学,2019:9
CHANG L F,LI X,GUO H J,et al. Genetic diversity analysis and comprehensive evaluation of octoploid Tritipyrumderived wheat breeding lines based on agronomic traits[J]. Acta Prataculturae Sinica,2022,31(11):61-74常利芳,李欣,郭慧娟,等. 小偃麦衍生系表型遗传多样性分析及综合评价[J]. 草业学报,2022,31(11):61-74
PEI X S. A comparative analysis between the path coefficient and relative hereditability[J]. Hereditas,1982,4(2):28-30裴新澍. 通径系数与相对遗传力的比较分析[J]. 遗传,1982,4(2):28-30
UPADHYAYA H D,SHARMA S,RAMULU B,et al. Variation for qualitative and quantitative traits and identification of trait-specific sources in new sorghum germplasm[J]. Crop & Pasture Science,2010,61(8):609-618
LIU D L,WANG X Q,LI W S,et al. Genetic diversity analysis of the phenotypic traits of 215 sugar beet germplasm resources[J]. Sugar Tech,2022,24(6):1790-1800
GUO Y D,LIU L Y,YUE Y S,et al. Development of SSR markers based on transcriptome sequencing and verification of their conservation across species of ornamental Pennisetum rich. (Poaceae)[J]. Agronomy,2022,12(7):1683-1683
DU H Q,FENG C S,LÜ X Z,et al. Evaluation and analysis of grass yield and related agronomic traits of 87 alfalfa varieties[J].Acta Ecologae Animalis Domastici,2025,46(5):90-99杜红旗,冯长松,吕先召,等. 87个苜蓿品种草产量及其相关农艺性状评价分析[J]. 家畜生态学报,2025,46(5):90-99
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LLOVERAS J,FERRAN J,ALVAREZ A,et al. Harvest management effects on alfalfa (Medicago sativaL.) production and quality in Mediterranean areas[J]. Grass and Forage Science,1998,53(1):88-92
SENGUL S. Yield components,morphology and forage quality of native alfalfa ecotypes[J]. Journal of Biological Sciences,2002,2(7):494-498
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doi: 10.11733/j.issn.1007-0435.2026.08.021
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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