Article(id=1309575001800532329, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.08.018, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1765728000000, receivedDateStr=2025-12-15, revisedDate=1768752000000, revisedDateStr=2026-01-19, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156611155, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156611155, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156611155, creator=13701087609, updateTime=1790156611155, updator=13701087609, issue=Issue{id=1309574965775659485, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='8', pageStart='2753', pageEnd='3140', issueExtLink='null', onlineDate='null', pubDate='1786723200000', pubDateStr='2026-08-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156602567, creator='13701087609', updateTime=1790156935415, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576361992352087, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576361992352088, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2946, endPage=2959, ext={EN=ArticleExt(id=1309575002618421611, articleId=1309575001800532329, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Evaluation of Low Nitrogen Tolerance in Different Germplasm Resources of Elymus Species, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=The genus Elymus is an important native grass species for ecological restoration. Screening low nitrogen-tolerant germination germplasm and improving nitrogen use efficiency are one of the effective approaches for near-natural restoration of degraded grasslands. In this study, twelve indicators during germination and seedling growth stages of 60 Elymus germplasms were measured under low nitrogen (0.01 mmol·L-1) and control (1.00 mmol·L-1)conditions using the petri dish germination method. Their responses to low nitrogen were explored, and comprehensive evaluation of low nitrogen tolerance was conducted by combining membership function and systematic clustering analysis. The results showed that under low nitrogen stress, all the indicators of the germplasms were decreased. The average germination rate, germination vigor, germination index, seedling length, root length, seedling fresh weight, root fresh weight, total fresh weight, seedling dry weight, root dry weight, total dry weight, and root-shoot ratio decreased by 5.95%, 12.07%, 8.21%, 6.40%, 14.80%, 29.04%, 36.28%, 25.54%, 30.00%, 40.74%, 28.77% and 8.08%, respectively. There were significant differences in low nitrogen tolerance among the different germplasms. Seven germplasms, including E22, E58and E47, had a comprehensive low nitrogen tolerance coefficient exceeding 0.850, while E32 and E49 were below 0.66. Based on the low nitrogen tolerance coefficient, cluster analysis divided the tested germplasms into six categories: super-strong, medium-strong, low-strong, low-weak, medium-weak, and super-weak. Among them, 10 materials, including E47 and E22, were classified as super-strong low nitrogen-tolerant germplasms, and 3 materials, including E49 and E32, were classified as super-weak low nitrogen-tolerant germplasms. This study provides excellent parental materials for the mining and functional research of candidate genes related to Elymus low nitrogen tolerance, genetic improvement, and breeding of new varieties., authors=PAN Gui-fang1, SUN Zong-jiu1,2,3, FEI Rui-hua1, GUO Jin-hua1, LI Pei-ying1,2,3, Astaken·GULHAITI1, ZHANG Wen-long1, HU Die1, REN Meng-long1, MA Peng1, authorsList=PAN Gui-fang, SUN Zong-jiu, FEI Rui-hua, GUO Jin-hua, LI Pei-ying, Astaken·GULHAITI, ZHANG Wen-long, HU Die, REN Meng-long, MA Peng, authorCompany=1 Grassland College, Xinjiang Agricultural University, Urumqi, Xinjiang Province 830052, China;
2 Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang Province 830052, China;
3 Key Laboratory of Grassland Resources and Ecology of the Ministry of Education of the Western Arid Zone, Urumqi, Xinjiang Province 830052, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575002274488682, articleId=1309575001800532329, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=披碱草属不同种质资源耐低氮评价, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=披碱草属(Elymus)是重要的生态修复乡土草种,筛选耐低氮萌发种质,提高氮素利用效率,是退化草地近自然恢复的有效途径之一。本研究采用培养皿发芽法对低氮(0.01 mmol·L⁻¹)及对照(1.00 mmol·L⁻¹)下60份披碱草属种质12项萌发及幼苗生长指标进行测定,探讨其对低氮的响应差异,并结合隶属函数及系统聚类分析进行了耐低氮性综合评价。结果表明:低氮胁迫下所有种质的各项指标均呈下降趋势,其平均发芽率、发芽势、发芽指数、苗长、根长、苗鲜重、根鲜重、整体鲜重、苗干重、根干重、整体干重以及根冠比依次下降5.95%,12.07%,8.21%,6.40%,14.80%,29.04%,36.28%,25.54%,30.00%,40.74%,28.77%和8.08%。不同种质间耐低氮能力差异显著,E22,E58,E47等7份种质综合耐低氮系数超过0.850,而E32和E49低于0.66,基于耐低氮系数的聚类分析将供试种质划分为超强、中强、低强、低弱、中弱、超弱6类,其中超强耐低氮种质包括E47,E22等10份材料,超弱耐低氮种质包括E49,E32等3份种质。本研究为披碱草耐低氮性候选基因挖掘与功能研究、遗传改良及新品种的选育提供了优异亲本材料。, authors=潘桂芳1, 孙宗玖1,2,3, 费瑞华1, 郭锦华1, 李培英1,2,3, 阿斯太肯·居力海提1, 张文龙1, 胡蝶1, 任梦龙1, 马鹏1, authorsList=潘桂芳, 孙宗玖, 费瑞华, 郭锦华, 李培英, 阿斯太肯·居力海提, 张文龙, 胡蝶, 任梦龙, 马鹏, authorCompany=1 新疆农业大学草业学院, 新疆 乌鲁木齐 830052;
2 新疆草地资源与生态自治区重点实验室, 新疆 乌鲁木齐 830052;
3 西部干旱荒漠区草地资源与生态教育部重点实验室, 新疆 乌鲁木齐 830052, correspAuthors=null, authorNote=潘桂芳(1997-),女,汉族,甘肃武威人,硕士研究生,主要从事草地资源利用与植物保护、草种质资源创新与品种选育科学研究,E-mail:1877277192@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=JBcarvIBpCYJ9K68I6k9VA==, pdfFileSize=2065998, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=新疆维吾尔自治区重点研发项目 (2023B02031))}, authors=null, keywords=[Keyword(id=1309575006166802799, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575001800532329, language=CN, orderNo=1, keyword=披碱草), Keyword(id=1309575006288437616, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575001800532329, language=CN, orderNo=2, keyword=种子萌发), Keyword(id=1309575006368129393, 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HUANG W. Analysis of phenotypic genetic diversity and comprehensive evaluation of Elymus L.germplasm resources[D]. Yinchuan:Ningxia University,2022:1-2黄薇. 披碱草属种质资源表型遗传多样性分析与综合评价[D]. 银川:宁夏大学,2022:1-2
NTAKIRUTIMANA F, XIAO B W, XIE W G,et al. Potential effects of awn length variation on seed yield and components, seed dispersal and germination performance in Siberian wildrye (Elymus sibiricus L.) [J]. Plants,2019,8(12):561
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ZHANG Y C,YU H,WEI X X,et al. Analysis of Inflorescence types of Elymus species from Alpine regions with branched spike germplasm resources[J]. Journal of Plant Genetic Resources, 2024, 25(10): 1697-1712张永超,于辉,魏小星,等. 高寒区披碱草属花序分支种质资源穗型特征分析[J]. 植物遗传资源学报,2024,25(10):1697-1712
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ZHOU L L,PING W C,LIU Y L,et al. Response of Foxtail Millet seedling traits to low nitrogen stress and screening and evaluation of cultivars with low nitrogen tolerance[J]. Jiangsu Agricultural Sciences, 2025, 53(1):164-168周璐璐,平文超,刘艳丽,等. 谷子苗期性状对低氮胁迫的响应及耐低氮品种的筛选与评价[J].江苏农业科学,2025,53(1):164-168
SUN W Y, LV Y, LI Y C,et al. Comprehensive evaluation of cold resistance of 21 wild Elymus germplasms in Xizang[J/OL]. Pratacultural Science,2025,12(12):3141-3152孙婉玉,吕晔,李阳晨,等. 21份西藏野生披碱草属种质抗寒性综合评价[J]. 草业科学,2025,12(12):3141-3152
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CHEN X,JIAO T,MU R,et al. Effects of nitrogen addition on growth and photosynthetic characteristics of Elymus nutans in Alpine artificial grassland[J]. Acta Agrestia Sinica,2022,30(8):1964-1971陈鑫,焦婷,牧仁,等. 氮添加对高寒人工草地垂穗披碱草(Elymus nutans)生长及光合特性的影响[J]. 草地学报,2022,30(8):1964-1971
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LV Y Z,NIE Y T,ZHANG Z,et al. Comprehensive evaluation of seed storability of alfalfa and screening of key indicators[J]. Acta Agrestia Sinica,2024,32(4):1131-1141吕艳贞,聂宇婷,张昭,等. 紫花苜蓿种子耐贮藏性综合评价及关键指标筛选[J]. 草地学报,2024,32(4):1131-1141
IQBAL A,DONG Q,WANG X R,et al. N-efficient cotton genotype grown under low nitrogen shows relatively large root system,high biomass accumulation and nitrogen metabolism[J]. Agronomy Journal,2022,114(1):582-600
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草地学报 | 研究论文 2026,34(8): 2946-2959
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草地学报 |研究论文 2026 , 34 (8) : 2946 -2959
披碱草属不同种质资源耐低氮评价
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潘桂芳1, 孙宗玖1,2,3, 费瑞华1, 郭锦华1, 李培英1,2,3, 阿斯太肯·居力海提1, 张文龙1, 胡蝶1, 任梦龙1, 马鹏1
作者信息
    1 新疆农业大学草业学院, 新疆 乌鲁木齐 830052;
    2 新疆草地资源与生态自治区重点实验室, 新疆 乌鲁木齐 830052;
    3 西部干旱荒漠区草地资源与生态教育部重点实验室, 新疆 乌鲁木齐 830052
作者简介:
潘桂芳(1997-),女,汉族,甘肃武威人,硕士研究生,主要从事草地资源利用与植物保护、草种质资源创新与品种选育科学研究,E-mail:1877277192@qq.com
Evaluation of Low Nitrogen Tolerance in Different Germplasm Resources of Elymus Species
  • PAN Gui-fang1, SUN Zong-jiu1,2,3, FEI Rui-hua1, GUO Jin-hua1, LI Pei-ying1,2,3, Astaken·GULHAITI1, ZHANG Wen-long1, HU Die1, REN Meng-long1, MA Peng1
  • Affiliations
      1 Grassland College, Xinjiang Agricultural University, Urumqi, Xinjiang Province 830052, China;
      2 Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang Province 830052, China;
      3 Key Laboratory of Grassland Resources and Ecology of the Ministry of Education of the Western Arid Zone, Urumqi, Xinjiang Province 830052, China
    doi: 10.11733/j.issn.1007-0435.2026.08.018
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    披碱草属(Elymus)是重要的生态修复乡土草种,筛选耐低氮萌发种质,提高氮素利用效率,是退化草地近自然恢复的有效途径之一。本研究采用培养皿发芽法对低氮(0.01 mmol·L⁻¹)及对照(1.00 mmol·L⁻¹)下60份披碱草属种质12项萌发及幼苗生长指标进行测定,探讨其对低氮的响应差异,并结合隶属函数及系统聚类分析进行了耐低氮性综合评价。结果表明:低氮胁迫下所有种质的各项指标均呈下降趋势,其平均发芽率、发芽势、发芽指数、苗长、根长、苗鲜重、根鲜重、整体鲜重、苗干重、根干重、整体干重以及根冠比依次下降5.95%,12.07%,8.21%,6.40%,14.80%,29.04%,36.28%,25.54%,30.00%,40.74%,28.77%和8.08%。不同种质间耐低氮能力差异显著,E22,E58,E47等7份种质综合耐低氮系数超过0.850,而E32和E49低于0.66,基于耐低氮系数的聚类分析将供试种质划分为超强、中强、低强、低弱、中弱、超弱6类,其中超强耐低氮种质包括E47,E22等10份材料,超弱耐低氮种质包括E49,E32等3份种质。本研究为披碱草耐低氮性候选基因挖掘与功能研究、遗传改良及新品种的选育提供了优异亲本材料。
    披碱草  /  种子萌发  /  耐低氮性  /  综合评价  /  聚类分析
    The genus Elymus is an important native grass species for ecological restoration. Screening low nitrogen-tolerant germination germplasm and improving nitrogen use efficiency are one of the effective approaches for near-natural restoration of degraded grasslands. In this study, twelve indicators during germination and seedling growth stages of 60 Elymus germplasms were measured under low nitrogen (0.01 mmol·L-1) and control (1.00 mmol·L-1)conditions using the petri dish germination method. Their responses to low nitrogen were explored, and comprehensive evaluation of low nitrogen tolerance was conducted by combining membership function and systematic clustering analysis. The results showed that under low nitrogen stress, all the indicators of the germplasms were decreased. The average germination rate, germination vigor, germination index, seedling length, root length, seedling fresh weight, root fresh weight, total fresh weight, seedling dry weight, root dry weight, total dry weight, and root-shoot ratio decreased by 5.95%, 12.07%, 8.21%, 6.40%, 14.80%, 29.04%, 36.28%, 25.54%, 30.00%, 40.74%, 28.77% and 8.08%, respectively. There were significant differences in low nitrogen tolerance among the different germplasms. Seven germplasms, including E22, E58and E47, had a comprehensive low nitrogen tolerance coefficient exceeding 0.850, while E32 and E49 were below 0.66. Based on the low nitrogen tolerance coefficient, cluster analysis divided the tested germplasms into six categories: super-strong, medium-strong, low-strong, low-weak, medium-weak, and super-weak. Among them, 10 materials, including E47 and E22, were classified as super-strong low nitrogen-tolerant germplasms, and 3 materials, including E49 and E32, were classified as super-weak low nitrogen-tolerant germplasms. This study provides excellent parental materials for the mining and functional research of candidate genes related to Elymus low nitrogen tolerance, genetic improvement, and breeding of new varieties.
    Elymus  /  Seed germination  /  Low nitrogen tolerance  /  Comprehensive evaluation  /  Cluster analysis
    潘桂芳, 孙宗玖, 费瑞华, 郭锦华, 李培英, 阿斯太肯·居力海提, 张文龙, 胡蝶, 任梦龙, 马鹏. 披碱草属不同种质资源耐低氮评价. 草地学报, 2026 , 34 (8) : 2946 -2959 . DOI: 10.11733/j.issn.1007-0435.2026.08.018
    PAN Gui-fang, SUN Zong-jiu, FEI Rui-hua, GUO Jin-hua, LI Pei-ying, Astaken·GULHAITI, ZHANG Wen-long, HU Die, REN Meng-long, MA Peng. Evaluation of Low Nitrogen Tolerance in Different Germplasm Resources of Elymus Species[J]. Acta Agrestia Sinica, 2026 , 34 (8) : 2946 -2959 . DOI: 10.11733/j.issn.1007-0435.2026.08.018

      新疆维吾尔自治区重点研发项目 (2023B02031)

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    SU Z G, HAN Y J, ZHAI Y J,et al. Research progress on germplasm resources of Elymus nutans[J]. Journal of Grassland and Forage Science,2025,(5):1-11,24苏志刚,韩有健,翟亚军,等. 垂穗披碱草种质资源研究进展[J]. 草学,2025,(5):1-11,24
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    ZHANG X Y,HE F F,ZHANG L,et al. Analysis of the morphologyl and physiological response of different Elymus dahuricus germplasm under low nitrogen stress[J]. Acta Agrestia Sinica,2024,32(7):2128-2142张心怡,何菲菲,张亮,等. 不同披碱草种质对低氮胁迫的形态和生理响应分析及耐低氮性评价[J]. 草地学报,2024,32(7):2128-2142
    LV Y Z,NIE Y T,ZHANG Z,et al. Comprehensive evaluation of seed storability of alfalfa and screening of key indicators[J]. Acta Agrestia Sinica,2024,32(4):1131-1141吕艳贞,聂宇婷,张昭,等. 紫花苜蓿种子耐贮藏性综合评价及关键指标筛选[J]. 草地学报,2024,32(4):1131-1141
    IQBAL A,DONG Q,WANG X R,et al. N-efficient cotton genotype grown under low nitrogen shows relatively large root system,high biomass accumulation and nitrogen metabolism[J]. Agronomy Journal,2022,114(1):582-600
    LIN Z R,LAI H J, FAN H H, et al. Effects of Nitrogen Deposition Simulation on Seed Germination of Cunninghamia lanceolata and Liquidambar formosana[J/OL]. https://link-cnki-net-s.webvpn.xjau.edu.cn/urlid/46.1068.S,2024-05-23/2025-11-15林智榕,赖慧捷,范辉华,等.氮沉降模拟对杉木和枫香种子萌发的影响[J/OL]. https://link-cnki-net-s.webvpn.xjau.edu.cn/urlid/46.1068.S,2024-05-23/2025-11-15
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    2026年第34卷第8期
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    doi: 10.11733/j.issn.1007-0435.2026.08.018
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    • 收稿日期:2025-12-15
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    2种不同金属材料的力学参数
    科
    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)
    属
    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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