Evaluation of Low Nitrogen Tolerance in Different Germplasm Resources of Elymus Species
PAN Gui-fang1, SUN Zong-jiu1,2,3, FEI Rui-hua1, GUO Jin-hua1, LI Pei-ying1,2,3, Astaken·GULHAITI1, ZHANG Wen-long1, HU Die1, REN Meng-long1, MA Peng1
Affiliations
1 Grassland College, Xinjiang Agricultural University, Urumqi, Xinjiang Province 830052, China; 2 Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang Province 830052, China; 3 Key Laboratory of Grassland Resources and Ecology of the Ministry of Education of the Western Arid Zone, Urumqi, Xinjiang Province 830052, China
The genus Elymus is an important native grass species for ecological restoration. Screening low nitrogen-tolerant germination germplasm and improving nitrogen use efficiency are one of the effective approaches for near-natural restoration of degraded grasslands. In this study, twelve indicators during germination and seedling growth stages of 60 Elymus germplasms were measured under low nitrogen (0.01 mmol·L-1) and control (1.00 mmol·L-1)conditions using the petri dish germination method. Their responses to low nitrogen were explored, and comprehensive evaluation of low nitrogen tolerance was conducted by combining membership function and systematic clustering analysis. The results showed that under low nitrogen stress, all the indicators of the germplasms were decreased. The average germination rate, germination vigor, germination index, seedling length, root length, seedling fresh weight, root fresh weight, total fresh weight, seedling dry weight, root dry weight, total dry weight, and root-shoot ratio decreased by 5.95%, 12.07%, 8.21%, 6.40%, 14.80%, 29.04%, 36.28%, 25.54%, 30.00%, 40.74%, 28.77% and 8.08%, respectively. There were significant differences in low nitrogen tolerance among the different germplasms. Seven germplasms, including E22, E58and E47, had a comprehensive low nitrogen tolerance coefficient exceeding 0.850, while E32 and E49 were below 0.66. Based on the low nitrogen tolerance coefficient, cluster analysis divided the tested germplasms into six categories: super-strong, medium-strong, low-strong, low-weak, medium-weak, and super-weak. Among them, 10 materials, including E47 and E22, were classified as super-strong low nitrogen-tolerant germplasms, and 3 materials, including E49 and E32, were classified as super-weak low nitrogen-tolerant germplasms. This study provides excellent parental materials for the mining and functional research of candidate genes related to Elymus low nitrogen tolerance, genetic improvement, and breeding of new varieties.
PAN Gui-fang, SUN Zong-jiu, FEI Rui-hua, GUO Jin-hua, LI Pei-ying, Astaken·GULHAITI, ZHANG Wen-long, HU Die, REN Meng-long, MA Peng.
Evaluation of Low Nitrogen Tolerance in Different Germplasm Resources of Elymus Species[J].
Acta Agrestia Sinica,
2026
, 34
(8)
: 2946
-2959
.
DOI: 10.11733/j.issn.1007-0435.2026.08.018
基金
收起
新疆维吾尔自治区重点研发项目 (2023B02031)
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