Article(id=1309574992157835833, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.08.012, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1773676800000, receivedDateStr=2026-03-17, revisedDate=1777219200000, revisedDateStr=2026-04-27, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156608857, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156608857, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156608857, creator=13701087609, updateTime=1790156608857, updator=13701087609, issue=Issue{id=1309574965775659485, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='8', pageStart='2753', pageEnd='3140', issueExtLink='null', onlineDate='null', pubDate='1786723200000', pubDateStr='2026-08-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156602567, creator='13701087609', updateTime=1790156935415, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576361992352087, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576361992352088, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2877, endPage=2888, ext={EN=ArticleExt(id=1309574992564683323, articleId=1309574992157835833, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Establishment of an Efficient Mutagenesis Breeding Technology System and Innovation of High Seed-Setting Germplasm in Leymus chinensis , columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To overcome the limitations of an imperfect mutagenesis breeding system for Leymus chinensis and the shortage of germplasm with high seed setting rates, this study established an efficient mutagenesis technology using a combination of spatial and atmospheric pressure room-temperature plasma (SP+ARTP). A high-throughput, accurate phenotypic analysis of large-scale mutant populations was achieved by utilizing an automatic seed setting rate identification model based on the YOLO algorithm. The results indicated that cultivar ‘Xiwuzhumuqin’ was the most suitable material for mutagenesis; a 60-minute treatment with 20% NaOH was the optimal pretreatment method. Single-space mutagenesis significantly promoted seed germination and seedling growth. The mutagenesis efficiency of the M1 generation using the SP+ARTP combination reached 1.20%, and 23 new germplasm lines with high seed setting rate were successfully selected. These lines exhibited a seed setting rate of 30% higher than that of the control group. The combined mutagenesis and high-throughput phenotyping system developed in this study provides key technical support and germplasm resources for the targeted breeding of high-seed-setting L. chinensis varieties and their industrial application., authors=SHI Yu-jie1 , ZI Zhi-lin2 , TANG Yuan-yuan1 , ZHU Meng-jiao2,3 , LIU Lei-zhen2 , CHEN Jun1 , authorsList=SHI Yu-jie, ZI Zhi-lin, TANG Yuan-yuan, ZHU Meng-jiao, LIU Lei-zhen, CHEN Jun, authorCompany=1 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; 2 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China; 3 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574992484991546, articleId=1309574992157835833, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=羊草高效诱变育种体系的建立与高结实率新种质创制, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为突破羊草(Leymus chinensis )诱变育种体系不完善与高结实率种质缺乏的瓶颈,本研究建立了空间与常压室温等离子体复合(SP+ARTP)的高效诱变技术体系。利用基于YOLO算法构建的结实率自动识别模型,实现了对大规模诱变群体表型的高通量、精准鉴定。结果表明,‘西乌珠穆沁’为最适诱变受体,20% NaOH处理60分钟为最佳前处理方法;单一空间诱变可显著促进种子萌发与幼苗生长;SP+ARTP复合诱变的M₁代诱变效率达1.20%,并成功筛选出23份高结实率新种质,其结实率较对照均提升30%以上。该研究所建立的复合诱变与高通量表型鉴定体系,为羊草高结实率品种的定向选育及产业化应用提供了关键技术支撑与种质资源。, authors=石玉杰1 , 訾芝琳2 , 唐媛媛1 , 朱梦娇2,3 , 刘雷震2 , 陈军1 , authorsList=石玉杰, 訾芝琳, 唐媛媛, 朱梦娇, 刘雷震, 陈军, authorCompany=1 中国农业大学农学院, 北京 100193; 2 中国农业大学草业科学与技术学院, 北京 100193; 3 中国医学科学院北京协和医学院药用植物研究所, 北京 100193, correspAuthors=null, authorNote=石玉杰(2000-),男,汉族,河北邢台人,博士研究生,主要从事饲草遗传育种研究,E-mail:1048971954@qq.com;訾芝琳(2002-),女,汉族,北京人,硕士研究生,主要从事草地智能管理研究,E-mail:Zhilin_zi@163.com;唐媛媛(2002-),女,汉族,重庆人,硕士研究生,主要从事饲草遗传育种研究,E-mail:2669705947@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=LPy+ti+/cboTeP6ttmZQQA==, pdfFileSize=11172138, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null)}, authors=null, keywords=[Keyword(id=1309574992862478908, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=CN, orderNo=1, keyword=羊草), Keyword(id=1309574992929587773, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=CN, orderNo=2, keyword=诱变育种), Keyword(id=1309574993017668158, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=CN, orderNo=3, keyword=复合诱变), Keyword(id=1309574994280153663, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=CN, orderNo=4, keyword=YOLO), Keyword(id=1309574994347262528, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=CN, orderNo=5, keyword=高结实率种质), Keyword(id=1309574994443731521, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=EN, orderNo=1, keyword=Leymus chinensis ), Keyword(id=1309574994556977730, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=EN, orderNo=2, keyword=Mutagenesis breeding), Keyword(id=1309574994624086595, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=EN, orderNo=3, keyword=Compound mutagenesis), Keyword(id=1309574994707972676, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=EN, orderNo=4, keyword=YOLO), Keyword(id=1309574994783470149, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574992157835833, language=EN, orderNo=5, keyword=High seed setting rate germplasm)], refs=null, funds=null, companyList=null, figs=null, attaches=null, journal=Journal(id=1308395841968504927, delFlag=0, nameCn=草地学报, nameEn=Acta Agrestia Sinica, nameHistory1=null, nameHistory2=null, issn=1007-0435, eissn=null, cn=11-3362/S, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=gCdi9sXCX82eLR6S1d4VLg==, journalPrice=null, startedYear=null, abbrevIsoEn=Acta Agrestia Sinica, journalRemark=null, publicationField=null, createdTime=1789875477545, updatedTime=1789876241473, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=C, firstLetterEn=C, subjectCode=Agriculture, subjectName=null, subjectCodeEn=Agriculture, subjectNameEn=null, picCn=gCdi9sXCX82eLR6S1d4VLg==, picEn=4dKqEDfsaGuyf+bpm/3gfw==, jcr=null, cjcr=null, exts=[JournalExt(id=1308399046192812446, language=CN, name=草地学报, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789876241491, updatedTime=1789876241491, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/authorLogOn.action, submissionEditorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/editorLogOn.action, submissionReviewUrl=https://manu40.magtech.com.cn/JournalX_cdxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1308399046268309919, language=EN, name=Acta Agrestia Sinica, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789876241509, updatedTime=1789876241509, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/authorLogOn.action, submissionEditorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/editorLogOn.action, submissionReviewUrl=https://manu40.magtech.com.cn/JournalX_cdxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1308397196074385509, websiteList=[Website(id=1308429426430071695, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1308397196074385509, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/cdxb/CN, language=CN, createTime=1789883484704, createBy=18614031015, updateTime=1789883745058, updateBy=18614031015, name=草地学报-中文, tplId=1146099689490845704, title=草地学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1308430669353340972, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=articleTextType, value=kx, createTime=1789883781040, updateTime=1789883781040, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669328175145, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=banner, value=null, createTime=1789883781034, updateTime=1789883781034, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669382701103, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=grayFlag, value=0, createTime=1789883781047, updateTime=1789883781047, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669319786536, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=logo, value=https://castjournals.cast.org.cn/joweb/cdxb/CN/file/pic?fileId=BZ28lIoKdB54MmA0z6K66g==, createTime=1789883781032, updateTime=1789883781032, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669395284017, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=minRunFlag, value=0, createTime=1789883781050, updateTime=1789883781050, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669344952363, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/cdxb/CN/file/pic, createTime=1789883781038, updateTime=1789883781038, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669391089712, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=silenceFlag, value=0, createTime=1789883781049, updateTime=1789883781049, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669336563754, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1789883781036, updateTime=1789883781036, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669361729581, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=themeColor, value=null, createTime=1789883781042, updateTime=1789883781042, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669370118190, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=themeStyle, value=null, createTime=1789883781044, updateTime=1789883781044, creator=18614031015, updator=18614031015)]), Website(id=1308429426501374870, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1308397196074385509, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/cdxb/EN, language=EN, createTime=1789883484721, createBy=18614031015, updateTime=1789883716139, updateBy=18614031015, name=草地学报-英文, tplId=1146101810881728533, title=Acta Agrestia Sinica, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1308430635979264030, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=articleTextType, value=kx, createTime=1789883773083, updateTime=1789883773083, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635954098203, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=banner, value=null, createTime=1789883773077, updateTime=1789883773077, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636000235553, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=grayFlag, value=0, createTime=1789883773088, updateTime=1789883773088, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635945709594, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=logo, value=https://castjournals.cast.org.cn/joweb/cdxb/EN/file/pic?fileId=BZ28lIoKdB54MmA0z6K66g==, createTime=1789883773075, updateTime=1789883773075, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636012818467, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=minRunFlag, value=0, createTime=1789883773091, updateTime=1789883773091, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635970875421, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/cdxb/EN/file/pic, createTime=1789883773081, updateTime=1789883773081, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636004429858, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=silenceFlag, value=0, createTime=1789883773089, updateTime=1789883773089, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635962486812, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1789883773079, updateTime=1789883773079, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635987652639, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=themeColor, value=null, createTime=1789883773085, updateTime=1789883773085, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635991846944, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=themeStyle, value=null, createTime=1789883773086, updateTime=1789883773086, creator=18614031015, updator=18614031015)])], journalTitle=草地学报, weixinUrl=null, journalUrl=https://manu40.magtech.com.cn/Jweb_cdxb, iacademicId=null, status=1, seqNo=null, journalTitleEn=Acta Agrestia Sinica, journalPhotoCn=gCdi9sXCX82eLR6S1d4VLg==, journalPhotoEn=4dKqEDfsaGuyf+bpm/3gfw==, journalFirstLetter=C, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/10.11733/j.issn.1007-0435.2026.08.012, detailUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/10.11733/j.issn.1007-0435.2026.08.012, pdfUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/PDF/10.11733/j.issn.1007-0435.2026.08.012, pdfUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/PDF/10.11733/j.issn.1007-0435.2026.08.012, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1786723200000, fullTextJson=null, articleText=null, reference=LIU G S,LI X X,QI D M,et al. Evaluation and utilization of Leymus chinensis germplasm resources[J]. Chinese Science Bulletin ,2016,61(2):271-281刘公社,李晓霞,齐冬梅,等. 羊草种质资源的评价与利用[J]. 科学通报,2016,61(2):271-281 LIU G S,WANG D L,SHI F L,et al. Review of study on germplasm resources of leymnus chinensis and its inspirations[J]. Chinese Journal of Grassland ,2022,44(4):1-9刘公社,王德利,石凤翎,等. 羊草种质资源研究历程及启示[J]. 中国草地学报,2022,44(4):1-9 DENG H X,WANG Y. Characteristics and field management of Leymus chinensis [J]. Technical Advisor for Animal Husbandry ,2014(9):254邓海霞,王影. 羊草的特性及田间管理[J]. 养殖技术顾问,2014(9):254 LI T,TANG S J,LI W,et al. Genome evolution and initial breeding of the Triticeae grass Leymus chinensis dominating the Eurasian Steppe[J]. Proceedings of the National Academy of Sciences of the United States of America ,2023,120(44):1-10 LIU G S,LI X P,WANG Y W. Research progress on the biology and resource utilization of Leymus chinensis [J]. Chinese Journal of Grassland ,2017,39(5):1-8刘公社,李晓平,王赟文. 羊草生物学与资源利用的研究进展[J]. 中国草地学报,2017,39(5):1-8 WANG X G,HAN L B,LI Q F. Current status and prospects of Leymus chinensis breeding[J]. Pratacultural Science ,2012,29(8):1307-1313王显国,韩烈保,李青丰. 羊草育种研究现状与展望[J]. 草业科学,2012,29(8):1307-1313 SUN S J,WANG J L,GUAN Y,et al. High-quality chromosome-level genomes reveal the structure and evolution of the S and Z self-incompatibility loci in Leymus chinensis [J]. Plant Biotechnology Journal ,2026,24(5):3279-3281 KIM Y, SCHUMAKER K S, ZHU J K. EMS mutagenesis of Arabidopsis [J]. Methods in Molecular Biology ,2006,323:101-103 ZHANG X,ZHANG X F,LI H P,et al. Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool[J]. Applied Microbiology and Biotechnology ,2014,98(12):5387-5396 LI G,LI H P,WANG L Y,et al. Genetic effects of radio-frequency,atmospheric-pressure glow discharges with helium[J]. Applied Physics Letters ,2008,92(22):221504 POETSCH A R. The genomics of oxidative DNA damage,repair,and resulting mutagenesis[J]. Computational and Structural Biotechnology Journal ,2020,18:207-219 LI R M,WANG J F,LI W J. Application of heavy ion beams to microbial mutation breeding and exploitation of biological energy[J]. Nuclear Physics Review ,2007,24(3):234-237李仁民,王菊芳,李文建. 重离子束在微生物诱变育种及生物能源开发中的应用[J]. 原子核物理评论,2007,24(3):234-237 WEN X F,ZHANG L,DAI W X,et al. Study of space mutation breeding in China[J]. Journal of Nuclear Agricultural Sciences ,2004,18(4):241-246温贤芳,张龙,戴维序,等. 天地结合开展我国空间诱变育种研究[J]. 核农学报,2004,18(4):241-246 HUANG Q C,LI Y F. Prospect of ion beam bio-technology in rice breeding[J]. Hybrid Rice ,2002,17(5):5-8黄群策,李玉峰. 离子束生物技术在水稻育种中的应用前景[J]. 杂交水稻,2002,17(5):5-8 SI J,ZHANG H,WU Z H. Applications and research progress of plant breeding with ion implantation technique[J]. Journal of Radiation Research and Radiation Processing ,2012,30(6):321-327司婧,张红,武振华. 离子注入技术在植物育种中的应用与研究进展[J]. 辐射研究与辐射工艺学报,2012,30(6):321-327 LUO H B,ZHAO K,ZHOU W X,et al. Genetic study on male sterile mutant of maize induced by heavy ion irradiation[J]. Acta Agriculturae Nucleatae Sinica ,2008,22(3):296-299罗红兵,赵葵,周文新,等. 重离子辐射诱导玉米雄性不育突变系的遗传研究[J]. 核农学报,2008,22(3):296-299 ZHANG H J,XIAO G,TAN T L,et al. High oleate material of rapeseed (Brassica napus ) produced by EMS treatment[J]. Scientia Agricultura Sinica ,2008,41(12):4016-4022张宏军,肖钢,谭太龙,等. EMS处理甘蓝型油菜(Brassica napus )获得高油酸材料[J]. 中国农业科学,2008,41(12):4016-4022 SELVAM Y A,ELANGAIMANNAN R,VENKATESAN M,et al. Chemically induced mutagenesis in Blackgram(Vigna mungo (L.)Hepper)[J]. Electronic Journal of Plant Breeding ,2010,1(4):921-924 WANG J,LIU R T,ZHANG Y W,et al. Effects of seeds carried by satellite on photosynthetic characteristics of Trifolium repens [J]. Pratacultural Science ,2010,27(12):107-112王健,刘荣堂,张蕴薇,等. 卫星搭载对白三叶光合特性的影响[J]. 草业科学,2010,27(12):107-112 YANG H S,WANG Y R,CHANG G Z,et al. A study on the space mutation of forages[J]. Chinese Journal of Grassland ,2015,37(1):104-110杨红善,王彦荣,常根柱,等. 牧草的航天诱变研究[J]. 中国草地学报,2015,37(1):104-110 KUANG Q,ZONG Y Q,YANG W,et al. The impacts of spatial mutagenesis on agronomic traits and nutrient quality of ‘Deqin’Alfalfa[J]. Acta Agrestia Sinica ,2024,32(6):1962-1967匡倩,宗亚倩,杨文,等. 空间诱变对‘德钦’紫花苜蓿农艺性状和营养品质的影响[J]. 草地学报,2024,32(6):1962-1967 SHEN X H. Analysis of DNA methylation map of alfalfa whole genome under two kinds of mutagenesis treatments[J]. Acta Agrestia Sinica ,2022,30(1):46-54申晓慧. 两种诱变处理下紫花苜蓿全基因组DNA甲基化图谱分析[J]. 草地学报,2022,30(1):46-54 GU Q Y, LIU W K, LIU M Y, et al. Research progress and prospects of space mutation breeding in forage and turfgrass [J/OL]. https://link.cnki.net/urlid/62.1156.s.20250929.2048.002, 2025-09-30/2026-04-27顾启元,刘维康,刘梦亚,等.牧草及草坪草航天诱变育种研究进展及展望[J/OL].https://link.cnki.net/urlid/62.1156.s.20250929.2048.002,2025-09-30/2026-04-27)
草地学报
|研究论文
2026
, 34
(8) :
2877
-2888
羊草高效诱变育种体系的建立与高结实率新种质创制
全屏
石玉杰1 , 訾芝琳2 , 唐媛媛1 , 朱梦娇2,3 , 刘雷震2 , 陈军1
作者信息
1 中国农业大学农学院, 北京 100193; 2 中国农业大学草业科学与技术学院, 北京 100193; 3 中国医学科学院北京协和医学院药用植物研究所, 北京 100193
作者简介:
石玉杰(2000-),男,汉族,河北邢台人,博士研究生,主要从事饲草遗传育种研究,E-mail:1048971954@qq.com;訾芝琳(2002-),女,汉族,北京人,硕士研究生,主要从事草地智能管理研究,E-mail:Zhilin_zi@163.com;唐媛媛(2002-),女,汉族,重庆人,硕士研究生,主要从事饲草遗传育种研究,E-mail:2669705947@qq.com
Establishment of an Efficient Mutagenesis Breeding Technology System and Innovation of High Seed-Setting Germplasm in Leymus chinensis
SHI Yu-jie1 , ZI Zhi-lin2 , TANG Yuan-yuan1 , ZHU Meng-jiao2,3 , LIU Lei-zhen2 , CHEN Jun1
Affiliations
1 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; 2 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China; 3 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
doi: 10.11733/j.issn.1007-0435.2026.08.012
文章导航
为突破羊草(Leymus chinensis )诱变育种体系不完善与高结实率种质缺乏的瓶颈,本研究建立了空间与常压室温等离子体复合(SP+ARTP)的高效诱变技术体系。利用基于YOLO算法构建的结实率自动识别模型,实现了对大规模诱变群体表型的高通量、精准鉴定。结果表明,‘西乌珠穆沁’为最适诱变受体,20% NaOH处理60分钟为最佳前处理方法;单一空间诱变可显著促进种子萌发与幼苗生长;SP+ARTP复合诱变的M₁代诱变效率达1.20%,并成功筛选出23份高结实率新种质,其结实率较对照均提升30%以上。该研究所建立的复合诱变与高通量表型鉴定体系,为羊草高结实率品种的定向选育及产业化应用提供了关键技术支撑与种质资源。
羊草
/
诱变育种
/
复合诱变
/
YOLO
/
高结实率种质
To overcome the limitations of an imperfect mutagenesis breeding system for Leymus chinensis and the shortage of germplasm with high seed setting rates, this study established an efficient mutagenesis technology using a combination of spatial and atmospheric pressure room-temperature plasma (SP+ARTP). A high-throughput, accurate phenotypic analysis of large-scale mutant populations was achieved by utilizing an automatic seed setting rate identification model based on the YOLO algorithm. The results indicated that cultivar ‘Xiwuzhumuqin’ was the most suitable material for mutagenesis; a 60-minute treatment with 20% NaOH was the optimal pretreatment method. Single-space mutagenesis significantly promoted seed germination and seedling growth. The mutagenesis efficiency of the M1 generation using the SP+ARTP combination reached 1.20%, and 23 new germplasm lines with high seed setting rate were successfully selected. These lines exhibited a seed setting rate of 30% higher than that of the control group. The combined mutagenesis and high-throughput phenotyping system developed in this study provides key technical support and germplasm resources for the targeted breeding of high-seed-setting L. chinensis varieties and their industrial application.
Leymus chinensis
/
Mutagenesis breeding
/
Compound mutagenesis
/
YOLO
/
High seed setting rate germplasm
石玉杰, 訾芝琳, 唐媛媛, 朱梦娇, 刘雷震, 陈军.
羊草高效诱变育种体系的建立与高结实率新种质创制.
草地学报,
2026
, 34
(8)
: 2877
-2888
.
DOI: 10.11733/j.issn.1007-0435.2026.08.012
SHI Yu-jie, ZI Zhi-lin, TANG Yuan-yuan, ZHU Meng-jiao, LIU Lei-zhen, CHEN Jun.
Establishment of an Efficient Mutagenesis Breeding Technology System and Innovation of High Seed-Setting Germplasm in Leymus chinensis [J].
Acta Agrestia Sinica ,
2026
, 34
(8)
: 2877
-2888
.
DOI: 10.11733/j.issn.1007-0435.2026.08.012
参考文献
引证文献
LIU G S,LI X X,QI D M,et al. Evaluation and utilization of Leymus chinensis germplasm resources[J]. Chinese Science Bulletin ,2016,61(2):271-281刘公社,李晓霞,齐冬梅,等. 羊草种质资源的评价与利用[J]. 科学通报,2016,61(2):271-281 LIU G S,WANG D L,SHI F L,et al. Review of study on germplasm resources of leymnus chinensis and its inspirations[J]. Chinese Journal of Grassland ,2022,44(4):1-9刘公社,王德利,石凤翎,等. 羊草种质资源研究历程及启示[J]. 中国草地学报,2022,44(4):1-9 DENG H X,WANG Y. Characteristics and field management of Leymus chinensis [J]. Technical Advisor for Animal Husbandry ,2014(9):254邓海霞,王影. 羊草的特性及田间管理[J]. 养殖技术顾问,2014(9):254 LI T,TANG S J,LI W,et al. Genome evolution and initial breeding of the Triticeae grass Leymus chinensis dominating the Eurasian Steppe[J]. Proceedings of the National Academy of Sciences of the United States of America ,2023,120(44):1-10 LIU G S,LI X P,WANG Y W. Research progress on the biology and resource utilization of Leymus chinensis [J]. Chinese Journal of Grassland ,2017,39(5):1-8刘公社,李晓平,王赟文. 羊草生物学与资源利用的研究进展[J]. 中国草地学报,2017,39(5):1-8 WANG X G,HAN L B,LI Q F. Current status and prospects of Leymus chinensis breeding[J]. Pratacultural Science ,2012,29(8):1307-1313王显国,韩烈保,李青丰. 羊草育种研究现状与展望[J]. 草业科学,2012,29(8):1307-1313 SUN S J,WANG J L,GUAN Y,et al. High-quality chromosome-level genomes reveal the structure and evolution of the S and Z self-incompatibility loci in Leymus chinensis [J]. Plant Biotechnology Journal ,2026,24(5):3279-3281 KIM Y, SCHUMAKER K S, ZHU J K. EMS mutagenesis of Arabidopsis [J]. Methods in Molecular Biology ,2006,323:101-103 ZHANG X,ZHANG X F,LI H P,et al. Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool[J]. Applied Microbiology and Biotechnology ,2014,98(12):5387-5396 LI G,LI H P,WANG L Y,et al. Genetic effects of radio-frequency,atmospheric-pressure glow discharges with helium[J]. Applied Physics Letters ,2008,92(22):221504 POETSCH A R. The genomics of oxidative DNA damage,repair,and resulting mutagenesis[J]. Computational and Structural Biotechnology Journal ,2020,18:207-219 LI R M,WANG J F,LI W J. Application of heavy ion beams to microbial mutation breeding and exploitation of biological energy[J]. Nuclear Physics Review ,2007,24(3):234-237李仁民,王菊芳,李文建. 重离子束在微生物诱变育种及生物能源开发中的应用[J]. 原子核物理评论,2007,24(3):234-237 WEN X F,ZHANG L,DAI W X,et al. Study of space mutation breeding in China[J]. Journal of Nuclear Agricultural Sciences ,2004,18(4):241-246温贤芳,张龙,戴维序,等. 天地结合开展我国空间诱变育种研究[J]. 核农学报,2004,18(4):241-246 HUANG Q C,LI Y F. Prospect of ion beam bio-technology in rice breeding[J]. Hybrid Rice ,2002,17(5):5-8黄群策,李玉峰. 离子束生物技术在水稻育种中的应用前景[J]. 杂交水稻,2002,17(5):5-8 SI J,ZHANG H,WU Z H. Applications and research progress of plant breeding with ion implantation technique[J]. Journal of Radiation Research and Radiation Processing ,2012,30(6):321-327司婧,张红,武振华. 离子注入技术在植物育种中的应用与研究进展[J]. 辐射研究与辐射工艺学报,2012,30(6):321-327 LUO H B,ZHAO K,ZHOU W X,et al. Genetic study on male sterile mutant of maize induced by heavy ion irradiation[J]. Acta Agriculturae Nucleatae Sinica ,2008,22(3):296-299罗红兵,赵葵,周文新,等. 重离子辐射诱导玉米雄性不育突变系的遗传研究[J]. 核农学报,2008,22(3):296-299 ZHANG H J,XIAO G,TAN T L,et al. High oleate material of rapeseed (Brassica napus ) produced by EMS treatment[J]. Scientia Agricultura Sinica ,2008,41(12):4016-4022张宏军,肖钢,谭太龙,等. EMS处理甘蓝型油菜(Brassica napus )获得高油酸材料[J]. 中国农业科学,2008,41(12):4016-4022 SELVAM Y A,ELANGAIMANNAN R,VENKATESAN M,et al. Chemically induced mutagenesis in Blackgram(Vigna mungo (L.)Hepper)[J]. Electronic Journal of Plant Breeding ,2010,1(4):921-924 WANG J,LIU R T,ZHANG Y W,et al. Effects of seeds carried by satellite on photosynthetic characteristics of Trifolium repens [J]. Pratacultural Science ,2010,27(12):107-112王健,刘荣堂,张蕴薇,等. 卫星搭载对白三叶光合特性的影响[J]. 草业科学,2010,27(12):107-112 YANG H S,WANG Y R,CHANG G Z,et al. A study on the space mutation of forages[J]. Chinese Journal of Grassland ,2015,37(1):104-110杨红善,王彦荣,常根柱,等. 牧草的航天诱变研究[J]. 中国草地学报,2015,37(1):104-110 KUANG Q,ZONG Y Q,YANG W,et al. The impacts of spatial mutagenesis on agronomic traits and nutrient quality of ‘Deqin’Alfalfa[J]. Acta Agrestia Sinica ,2024,32(6):1962-1967匡倩,宗亚倩,杨文,等. 空间诱变对‘德钦’紫花苜蓿农艺性状和营养品质的影响[J]. 草地学报,2024,32(6):1962-1967 SHEN X H. Analysis of DNA methylation map of alfalfa whole genome under two kinds of mutagenesis treatments[J]. Acta Agrestia Sinica ,2022,30(1):46-54申晓慧. 两种诱变处理下紫花苜蓿全基因组DNA甲基化图谱分析[J]. 草地学报,2022,30(1):46-54 GU Q Y, LIU W K, LIU M Y, et al. Research progress and prospects of space mutation breeding in forage and turfgrass [J/OL]. https://link.cnki.net/urlid/62.1156.s.20250929.2048.002, 2025-09-30/2026-04-27顾启元,刘维康,刘梦亚,等.牧草及草坪草航天诱变育种研究进展及展望[J/OL].https://link.cnki.net/urlid/62.1156.s.20250929.2048.002,2025-09-30/2026-04-27
2026年第34卷第8期
PDF下载
118
25
引用本文
BibTeX
文章信息
doi: 10.11733/j.issn.1007-0435.2026.08.012
接收时间:2026-03-17
首发时间:2026-09-23
收稿日期:2026-03-17
修回日期:2026-04-27
https://castjournals.cast.org.cn/joweb/cdxb/CN/10.11733/j.issn.1007-0435.2026.08.012
复制链接
引用本文
BibTeX
2种不同金属材料的力学参数
科 Family 属数 Number of genus 种数 Number of species 占总种数比例 Percentage of total species (%) 属 Genus 种数 Number of species 占总种数比例 Percentage of total species (%) 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
关闭全屏
BibTeX
EndNote
RefWorks
TxT