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2 Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou, Hebei Province 061000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574981449777691, articleId=1309574979520397849, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=紫花苜蓿EARLY RESPONSIVE TO DEHYDRATION 15基因克隆、表达及功能分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=ERD15（EARLY RESPONSIVE TO DEHYDRATION 15）是植物逆境响应的重要调控因子。本研究克隆紫花苜蓿（Medicago sativa L.）MsERD15基因，并通过生物信息学、表达模式及功能分析探讨其抗逆作用。利用RT-PCR克隆MsERD15基因并分析蛋白理化性质、结构域及系统进化关系；qRT-PCR检测干旱、高盐及脱落酸（Abscisic acid， ABA）处理下的表达模式；构建MsERD15-YFP融合载体进行亚细胞定位；通过酵母异源表达验证抗逆功能。结果表明：MsERD15编码区全长402 bp，编码134个氨基酸；该基因受干旱、高盐及ABA显著诱导；蛋白定位于细胞质。酵母表达MsERD15可显著提高对高盐和聚乙二醇（Polyethylene glycol，PEG）胁迫的耐受性。MsERD15参与逆境响应，可作为紫花苜蓿抗逆分子育种的候选基因。, authors=张伟燕¹, 李云¹, 刘艳昆¹, 王皓¹, 刘青松², authorsList=张伟燕, 李云, 刘艳昆, 王皓, 刘青松, authorCompany=1 沧州职业技术学院, 河北 沧州 061000;
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