草地学报

不同放牧强度对短花针茅荒漠草原土壤酶活性的影响及其驱动因素

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草地学报 | 研究论文 2026,34(7): 2615-2623

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草地学报 |研究论文 2026 , 34 (7) : 2615 -2623

不同放牧强度对短花针茅荒漠草原土壤酶活性的影响及其驱动因素

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薛逸凡¹, 王宁斌², 李邵宇¹, 樊佳悦¹, 吕金岭¹, 茶俊香¹, 孙海淋¹, 张峰¹, 张彬¹, 赵萌莉¹

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作者简介:

薛逸凡（2005-），男，蒙古族，内蒙古锡林郭勒人，本科生，主要从事草地生态学研究，E-mail:nmgxueyifan@163.com

Effects of Different Grazing Intensities on Soil Enzyme Activities and Driving Factors in Stipa breviflora Desert Steppe

XUE Yi-fan¹, WANG Ning-bin², LI Shao-yu¹, FAN Jia-yue¹, LÜ Jin-ling¹, CHA Jun-xiang¹, SUN Hai-lin¹, ZHANG Feng¹, ZHANG Bin¹, ZHAO Meng-li¹

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doi: 10.11733/j.issn.1007-0435.2026.07.024

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为探讨不同放牧强度对短花针茅（Stipa breviflora）荒漠草原土壤酶活性的影响及其因素，本研究基于短花针茅荒漠草原，设置不放牧（No grazing， CK）、轻度放牧（Light grazing， LG）、中度放牧（Moderate grazing， MG）和重度放牧（Heavy grazing， HG）4个梯度，分析了不同放牧强度对β-D-纤维素酶、β-1，4-N-乙酰氨基葡萄糖苷酶和碱性磷酸酶活性的影响，并结合物种丰富度、地上净初级生产力及土壤pH值、饱和含水量、容重、有机碳、速效氮、速效磷等因子。结果表明：随放牧强度增加，三种土壤酶活性、物种丰富度、地上净初级生产力、土壤有机碳、速效氮及速效磷均显著降低（P<0.05），容重则显著上升（P<0.05）。随机森林模型显示，速效氮与速效磷是调控土壤酶活性的主要驱动因子。放牧主要通过降低速效养分含量抑制土壤酶活性。本研究为荒漠草原保护与生态恢复提供了理论依据，草地管理利用中可采取控制载畜率、季节性休牧等措施。

关键词

放牧强度 / 荒漠草原 / 土壤酶活性 / 物种丰富度

Abstract

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To investigate the effects of different grazing intensities on soil enzyme activities in a Stipa breviflora desert steppe and the driving factors， this study was based on a Stipa breviflora desert steppe and established four grazing intensity levels： no grazing （CK）， light grazing （LG）， moderate grazing （MG）， and heavy grazing （HG）. The effects of different grazing intensities on the activities of β-D-cellobiohydrolase， β-1，4-N-acetylglucosaminidase， and alkaline phosphatase were analyzed， along with species richness， aboveground net primary productivity （ANPP）， and soil pH， saturated water content， bulk density， organic carbon， available nitrogen， and available phosphorus. The results showed that with the increase of grazing intensity， the three soil enzyme activities， species richness， aboveground net primary productivity， soil organic carbon， available nitrogen， and available phosphorus all significantly decreased （P<0.05）， whereas soil bulk density was significantly increased. The Random Forest model indicated that available nitrogen and available phosphorus were the primary drivers regulating soil enzyme activities. Grazing suppresses soil enzyme activities primarily by decreasing available nutrients. This study provides a theoretical basis for the conservation and ecological restoration of desert steppes. In grassland management and utilization， measures such as controlling stocking rates and implementing seasonal grazing exclusion can be adopted.

Key words

Grazing intensity / Desert steppe / Soil enzyme activities / Species richness

引用本文

薛逸凡, 王宁斌, 李邵宇, 樊佳悦, 吕金岭, 茶俊香, 孙海淋, 张峰, 张彬, 赵萌莉. 不同放牧强度对短花针茅荒漠草原土壤酶活性的影响及其驱动因素. 草地学报, 2026 , 34 (7) : 2615 -2623 . DOI: 10.11733/j.issn.1007-0435.2026.07.024

XUE Yi-fan, WANG Ning-bin, LI Shao-yu, FAN Jia-yue, LÜ Jin-ling, CHA Jun-xiang, SUN Hai-lin, ZHANG Feng, ZHANG Bin, ZHAO Meng-li. Effects of Different Grazing Intensities on Soil Enzyme Activities and Driving Factors in Stipa breviflora Desert Steppe[J]. Acta Agrestia Sinica, 2026 , 34 (7) : 2615 -2623 . DOI: 10.11733/j.issn.1007-0435.2026.07.024

基金

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内蒙古自治区自然科学基金项目 (2024QN03049); 草学一流学科科研专项 (YLXKZX-NND-040); 内蒙古农业大学高层次人才引进科研项目 (NDYB2023-16); 内蒙古自治区草种质创新与草地资源可持续利用重点实验室 (2025KYPT0040); 中国科协青年人才托举工程项目资助

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SHI R X, WANG Q C, LIU N, et al. Long-term drainage reduces soil enzyme activities in Alpine peatlands on the zoige plateau[J]. Acta Agrestia Sinica, 2025, 33(5):1398-1407 时蓉喜, 王全成, 刘宁, 等. 长期排水降低若尔盖高寒泥炭地土壤酶活性[J]. 草地学报, 2025, 33(5):1398-1407
ZHANG H H, LI L H, CHEN D W, et al. Characteristics of soil enzyme activity and enzyme stoichiometric ratio in different types of Alpine grasslands in northern Xizang[J]. Acta Agrestia Sinica, 2025, 33(8):2482-2493 张欢欢, 李林昊, 陈德炜, 等. 藏北不同类型高寒草地土壤酶活性及酶化学计量比特征[J]. 草地学报, 2025, 33(8):2482-2493
WANG Y, XIA Z Q, WU X, et al. Effect of composite soil amendments on soil physicochemical properties and enzyme activities of rare earth tailings[J]. Acta Agriculturae Universitatis Jiangxiensis (Natural Sciences Edition), 2025, 47(1):255-266 王艳, 夏志强, 吴璇, 等. 复合土壤改良剂对稀土尾矿土壤理化性质及酶活性的影响[J]. 江西农业大学学报, 2025, 47(1):255-266
SI Y E, RUKEYANMU M, AIKEBAIER Y, et al. Effects of bio-organic fertilizer on quality of Lycium barbarum L. and soil enzyme activity[J]. Xinjiang Agricultural Sciences, 2025, 62(2):350-360 司艳娥, 如克艳木·买提司地克, 艾克拜尔·伊拉洪, 等. 生物有机肥对枸杞品质及土壤酶活性的影响[J]. 新疆农业科学, 2025, 62(2):350-360
ZHAO S, ZHANG J N, LAI X, et al. Effects of grazing and fencing on soil enzyme activity and soil physicochemical properties in Stipa steppe in Hulunbeier, Inner Mongolia[J]. Chinese Journal of Grassland, 2011, 33(1):71-76 赵帅, 张静妮, 赖欣, 等. 放牧与围封对呼伦贝尔针茅草原土壤酶活性及理化性质的影响[J]. 中国草地学报, 2011, 33(1):71-76
NANNIPIERI P, TRASAR-CEPEDA C, DICK R P. Soil enzyme activity: a brief history and biochemistry as a basis for appropriate interpretations and meta-analysis[J]. Biology and Fertility of Soils, 2018, 54(1):11-19
MENCEL J, MOCEK-PŁÓCINIAK A, KRYSZAK A. Soil microbial community and enzymatic activity of grasslands under different use practices: a review[J]. Agronomy, 2022, 12(5):1136
XU J Y, WANG Q H, MA C, et al. Impact of different artificial grass species on soil enzyme activity and enzyme stoichiometry in Alpine regions[J]. Research of Soil and Water Conservation, 2025, 32(1):73-81 徐嘉翊, 王巧红, 马铖, 等. 高寒地区不同人工牧草种类对土壤酶活性及酶化学计量比的影响[J]. 水土保持研究, 2025, 32(1):73-81
HUANG X D, LIU T, NIU H Z, et al. Effect of coal-based molecular sieve on improving physical and chemical properties of saline farmland and enhancing soil enzyme activity[J]. Applied Chemical Industry, 2025, 54(1):131-136, 146 黄旭迪, 刘甜, 牛浩泽, 等. 煤基分子筛改良盐碱农田土壤理化性质提升土壤酶活研究[J]. 应用化工, 2025, 54(1):131-136, 146
REYNOLDS J F, SMITH D M S, LAMBIN E F, et al. Global desertification: building a science for dryland development[J]. Science, 2007, 316(5826):847-851
WANG X D, YAN Y, CAO Y Z. Impact of historic grazing on steppe soils on the northern Tibetan Plateau[J]. Plant and Soil, 2012, 354(1):173-183
ZHENG S X, REN H Y, LI W H, et al. Scale-dependent effects of grazing on plant C: N: P stoichiometry and linkages to ecosystem functioning in the Inner Mongolia grassland[J]. PLoS One, 2012, 7(12):e51750
TRAVERS S K, ELDRIDGE D J, VAL J, et al. Rabbits and livestock grazing alter the structure and composition of mid-storey plants in a wooded dryland[J]. Agriculture, Ecosystems & Environment, 2019, 277:53-60
BAI Y F, LI D X, XU Z X, et al. Growth and reproduction of Stipa Krylovii population on a grazing gradient[J]. Acta Ecologica Sinica, 1999, 19(4):479-484 白永飞, 李德新, 许志信, 等. 牧压梯度对克氏针茅生长和繁殖的影响[J]. 生态学报, 1999, 19(4):479-484
WANG Q, ZHENG J H, ZHANG F, et al. Effects of grazing intensity on the ecological niche of plant population in Stipa breviflora desert steppe[J]. Chinese Journal of Grassland, 2022, 44(10):1-9 王琪, 郑佳华, 张峰, 等. 放牧强度对短花针茅荒漠草原植物种群生态位的影响[J]. 中国草地学报, 2022, 44(10):1-9
PEI J H, LI J W, HAN G D, et al. Response of plant functional traits to stocking rates in the Stipa breviflora desert steppe[J]. Chinese Journal of Grassland, 2025, 47(1):27-36 裴婧宏, 李江文, 韩国栋, 等. 短花针茅荒漠草原植物功能性状对载畜率的响应[J]. 中国草地学报, 2025, 47(1):27-36
SINSABAUGH R L, HILL B H, FOLLSTAD SHAH J J. Ecoenzymatic stoichiometry of microbial organic nutrient acquisition in soil and sediment[J]. Nature, 2009, 462(7274):795-798
BARDGETT R D, VAN DER PUTTEN W H. Belowground biodiversity and ecosystem functioning[J]. Nature, 2014, 515(7528):505-511
BURNS R G, DEFOREST J L, MARXSEN J, et al. Soil enzymes in a changing environment: current knowledge and future directions[J]. Soil Biology and Biochemistry, 2013, 58:216-234
ZHANG F, BENNETT J A, ZHANG B, et al. Cessation of grazing stabilizes productivity through effects on species asynchrony and stability of shrub/semi-shrub plants in arid grasslands[J]. Agriculture, Ecosystems & Environment, 2023, 348:108411
CHUN F, ZHANG F, WU Y S, et al. Effects of different grazing intensities on biodiversity in the Stipa brevi flora desert steppe[J]. Acta Agrestia Sinica, 2024, 32(3):848-858 春风, 张峰, 吴永胜, 等. 不同放牧强度对短花针茅荒漠草原生物多样性的影响[J]. 草地学报, 2024, 32(3):848-858
ZHANG F, GAO F, SUN J W, et al. Effects of different stocking rates on spatio-temporal variability of constructive species Stipa breviflora populations in the desert steppe[J]. Acta Agrestia Sinica, 2021, 29(6):1369-1374 张峰, 高峰, 孙嘉伟, 等. 不同载畜率对荒漠草原建群种短花针茅种群时空变异性的影响[J]. 草地学报, 2021, 29(6):1369-1374
CHEN H, LI D J, XIAO K C, et al. Soil microbial processes and resource limitation in karst and non-karst forests[J]. Functional Ecology, 2018, 32(5):1400-1409
JING X, CHEN X, FANG J Y, et al. Soil microbial carbon and nutrient constraints are driven more by climate and soil physicochemical properties than by nutrient addition in forest ecosystems[J]. Soil Biology and Biochemistry, 2020, 141:107657
SHAO M A, WANG Q J, HUANG M B. Soil physics[M]. Beijing: Higher Education Press, 2006:80-85 邵明安, 王全九, 黄明斌. 土壤物理学[M]. 北京:高等教育出版社, 2006:80-85
BAO S D. Soil and agricultural chemistry analysis[M]. 3rd ed. Beijing: China Agriculture Press, 2000:14-97 鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社, 2000:14-97
ZHAO M, ZHAO K, WANG Y B, et al. Long-term grazing disturbance reduced plant diversity in Stipa breviflora desert steppe[J]. Acta Prataculturae Sinica, 2023, 32(9):39-49 赵敏, 赵坤, 王赟博, 等. 长期放牧干扰降低了短花针茅荒漠草原植物多样性[J]. 草业学报, 2023, 32(9):39-49
WANG Z H, LÜ S J, WANG Z W, et al. Effects of grazing intensity on dominant population and species diversity and their typical relationships[J]. Ecology and Environmental Sciences, 2024, 33(6):869-876 王梓晗, 吕世杰, 王忠武, 等. 放牧强度对优势种群重要值和物种多样性及其二者典型关系的影响[J]. 生态环境学报, 2024, 33(6):869-876
WU J X, LI S Y, HAN G D. Study on soil carbon, nitrogen, phosphorus acquisition enzyme activity, and microbial entropy in Stipa breviflora desert grassland of Inner Mongolia[J]. Pratacultural Science, 2024, 41(10):2240-2249 吴佳芯, 李邵宇, 韩国栋. 内蒙古短花针茅荒漠草原土壤碳氮磷获取酶活性及微生物熵[J]. 草业科学, 2024, 41(10):2240-2249
FONTAINE S, BAROT S, BARRÉ P, et al. Stability of organic carbon in deep soil layers controlled by fresh carbon supply[J]. Nature, 2007, 450(7167):277-280
SINSABAUGH R L, LAUBER C L, WEINTRAUB M N, et al. Stoichiometry of soil enzyme activity at global scale[J]. Ecology Letters, 2008, 11(11):1252-1264
MA W J, LI J, GAO Y, et al. Responses of soil extracellular enzyme activities and microbial community properties to interaction between nitrogen addition and increased precipitation in a semi-arid grassland ecosystem[J]. Science of the Total Environment, 2020, 703:134691
WANG Z H, LÜ S J, YANG Y W, et al. Effects of grazing intensity on dominant populations and species diversity in a Stipa breviflora desert steppe[J]. Pratacultural Science, 2025, 42(3):591-600 王梓晗, 吕世杰, 杨溢文, 等. 放牧强度对短花针茅荒漠草原优势种群和物种多样性的影响[J]. 草业科学, 2025, 42(3):591-600
ZHU A M, LIU H L, WANG Y H, et al. Grazing intensity changed the activities of nitrogen assimilation related enzymes in desert Steppe Plants[J]. BMC Plant Biology, 2021, 21(1):436
FAN L L, LIANG Y Y, LI X F, et al. Grazing decreases soil aggregation and has different effects on soil organic carbon storage across different grassland types in northern Xinjiang, China[J]. Land, 2023, 12(8):1575
WANG Y Y, CHEN X C, DONG Z, et al. Effects of different grazing intensities on vegetation community characteristics and soil aggregates in Xilamuren grassland[J]. Journal of Soil and Water Conservation, 2024, 38(3):121-129, 139 王艺颖, 陈新闯, 董智, 等. 希拉穆仁草原不同放牧强度对植被群落特征和土壤团聚体的影响[J]. 水土保持学报, 2024, 38(3):121-129, 139
HARTMANN M, SIX J. Soil structure and microbiome functions in agroecosystems[J]. Nature Reviews Earth & Environment, 2023, 4(1):4-18
GAO J J, CARMEL Y. Can the intermediate disturbance hypothesis explain grazing-diversity relations at a global scale？[J]. Oikos, 2020, 129(4):493-502
YANG D L, HAN G D, HU Y G, et al. Effects of grazing intensity on plant diversity and aboveground biomass of Stipa baicalensis grassland[J]. Chinese Journal of Ecology, 2006, 25(12):1470-1475 杨殿林, 韩国栋, 胡跃高, 等. 放牧对贝加尔针茅草原群落植物多样性和生产力的影响[J]. 生态学杂志, 2006, 25(12):1470-1475
BAI Y F, HAN X G, WU J G, et al. Ecosystem stability and compensatory effects in the Inner Mongolia grassland[J]. Nature, 2004, 431(7005):181-184
LI Q, SUN Y N, LIN L, et al. Changes of soil enzyme activities and nutrients across different succession stages of grazing Alpine Kobresia grassland[J]. Chinese Journal of Applied Ecology, 2019, 30(7):2267-2274 李茜, 孙亚男, 林丽, 等. 放牧高寒嵩草草地不同演替阶段土壤酶活性及养分演变特征[J]. 应用生态学报, 2019, 30(7):2267-2274
MOOSHAMMER M, WANEK W, ZECHMEISTER-BOLTENSTERN S, et al. Stoichiometric imbalances between terrestrial decomposer communities and their resources: mechanisms and implications of microbial adaptations to their resources[J]. Frontiers in Microbiology, 2014, 5:22
WANG S Q, SONG M H, WANG C M, et al. Mechanisms underlying soil microbial regulation of available phosphorus in a temperate forest exposed to long-term nitrogen addition[J]. Science of the Total Environment, 2023, 904:166403
DU E Z, TERRER C, PELLEGRINI A F A, et al. Global patterns of terrestrial nitrogen and phosphorus limitation[J]. Nature Geoscience, 2020, 13(3):221-226
CUI Y X, ZHANG Y L, DUAN C J, et al. Ecoenzymatic stoichiometry reveals microbial phosphorus limitation decreases the nitrogen cycling potential of soils in semi-arid agricultural ecosystems[J]. Soil and Tillage Research, 2020, 197:104463
SUMNER J B. The isolation and crystallization of the enzyme urease preliminary paper[J]. Journal of Biological Chemistry, 1926, 69(2):435-441
JARAI G, BUXTON F. Nitrogen, carbon, and pH regulation of extracellular acidic proteases of Aspergillus niger[J]. Current Genetics, 1994, 26(3):238-244
ZHU H Y, JIN Z J, ZHANG X W, et al. Comparison of soil phosphorus content and alkaline phosphatase activity in paddy fields in karst and non-karst areas[J]. Journal of Agricultural Resources and Environment, 2024, 41(5):1083-1090 朱虹颖, 靳振江, 张晓文, 等. 岩溶区与非岩溶区稻田土壤磷含量与碱性磷酸酶活性比较[J]. 农业资源与环境学报, 2024, 41(5):1083-1090
CUI Y X, MOORHEAD D L, PENG S S, et al. New insights into the patterns of ecoenzymatic stoichiometry in soil and sediment[J]. Soil Biology and Biochemistry, 2023, 177:108910

2026年第34卷第7期

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doi: 10.11733/j.issn.1007-0435.2026.07.024

接收时间：2025-11-07
首发时间：2026-09-23

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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