Article(id=1309574791477162275, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574765791244449, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.07.021, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1764172800000, receivedDateStr=2025-11-27, revisedDate=1772035200000, revisedDateStr=2026-02-26, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156561011, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156561011, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156561011, creator=13701087609, updateTime=1790156561011, updator=13701087609, issue=Issue{id=1309574765791244449, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='7', pageStart='2341', pageEnd='2752', issueExtLink='null', onlineDate='null', pubDate='1784044800000', pubDateStr='2026-07-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156554887, creator='13701087609', updateTime=1790156849638, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576002150424823, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574765791244449, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576002154619128, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574765791244449, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2579, endPage=2591, ext={EN=ArticleExt(id=1309574791779152165, articleId=1309574791477162275, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Plowing and Nutrient Addition on Plant Community Diversity and Biomass in Alpine Meadows, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Plowing and fertilization are common management practices in alpine meadows, which directly influence plant productivity and species diversity. This study established experimental plots with plowing (Dis), nutrient addition (NPK), and their interactive treatment (DisNPK) at the Tianzhu Alpine Grassland Ecosystem Experimental Station of Gansu Agricultural University, with natural grassland as a control (CK). The objectives were to investigate the effects of plowing and nutrient addition on plant community structure and biomass in alpine meadows and to identify the key driving factors. By measuring soil physicochemical properties, plant biomass, and species diversity, we analyzed the relationships between soil physicochemical properties and vegetation biomass/species diversity. The results showed that all treatments significantly increased the contents of total and available soil nutrients compared to control. The NPK treatment significantly increased aboveground biomass, and the combined effect of plowing and fertilization (DisNPK) had the most significant enhancement on productivity (particularly for grasses and above-ground biomass). The Dis and DisNPK treatments significantly reduced community diversity. Correlation analysis revealed that soil available nutrients and organic matter were positively correlated with above-ground biomass but negatively correlated with diversity indices (P<0.05). This study demonstrates that plowing and nutrient addition can alter the community structure and productivity of the ecosystem, providing a theoretical basis for the ecological restoration of alpine meadows., authors=DONG Wen-wen1, CHEN Li-juan1, ZHANG Rui-xuan1, WANG Yi-ming1, YUE Lei1, YANG Jian1, SUN Hao1, ZHOU Jie1, XUE Ling-yu1, MENG Tian-le1, WEN Ai-fang1, CHEN Guang-jun1, MA Jing1, ZHANG Xiao-juan1, CHEN Xian-min1, BAI Yu-jie1, YANG Jie1, TANG Zhuang-sheng1, authorsList=DONG Wen-wen, CHEN Li-juan, ZHANG Rui-xuan, WANG Yi-ming, YUE Lei, YANG Jian, SUN Hao, ZHOU Jie, XUE Ling-yu, MENG Tian-le, WEN Ai-fang, CHEN Guang-jun, MA Jing, ZHANG Xiao-juan, CHEN Xian-min, BAI Yu-jie, YANG Jie, TANG Zhuang-sheng, authorCompany=1 College of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574791703654692, articleId=1309574791477162275, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=翻耕与养分添加对高寒草甸植物群落多样性及生物量的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=翻耕和施肥是高寒草甸的常见管理措施,直接影响植物生产力和物种多样性。本研究在甘肃农业大学天祝高山草原生态系统试验站设置翻耕(Dis)、养分添加(NPK)及二者交互处理(DisNPK)的实验样地,以自然草地为对照(CK)。通过测定土壤理化性质、植物生物量及物种多样性,分析土壤理化性质与植被生物量及物种多样性之间的关系,探究翻耕与养分添加对高寒草甸植物群落结构及生物量的影响,并探明其关键驱动因子。结果表明:所有处理均显著提高了土壤全效和速效养分含量;NPK 处理显著提高了地上生物量,当翻耕与施肥结合(DisNPK)时,对生产力(特别是禾本科与总生物量)的提升效果最显著;Dis和DisNPK处理显著降低了群落多样性。相关性分析显示,土壤速效养分和有机质与地上生物量正相关,而与多样性指数负相关(P<0.05)。本研究表明翻耕及养分添加会影响生态系统的群落结构和生产力,为高寒草甸生态修复提供理论依据。, authors=董雯雯1, 陈丽娟1, 张瑞轩1, 王一鸣1, 岳磊1, 杨健1, 孙浩1, 周杰1, 薛凌宇1, 孟天乐1, 文爱芳1, 陈广军1, 马晶1, 张晓娟1, 陈先敏1, 白宇杰1, 杨洁1, 唐庄生1, authorsList=董雯雯, 陈丽娟, 张瑞轩, 王一鸣, 岳磊, 杨健, 孙浩, 周杰, 薛凌宇, 孟天乐, 文爱芳, 陈广军, 马晶, 张晓娟, 陈先敏, 白宇杰, 杨洁, 唐庄生, authorCompany=1 甘肃农业大学草业学院, 甘肃 兰州 730070, correspAuthors=null, authorNote=董雯雯(2002-),女,汉族,甘肃兰州人,硕士研究生,主要从事草地生态研究,E-mail:dongww2024@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=Tcrfdtx3QtpwZkDqhs13+w==, pdfFileSize=3495043, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金 (32360345); 甘肃农业大学伏羲青年英才 (GAUfx-04Y06); 甘肃省杰出青年基金 (24JRRA632); 中国农业大学对口支援项目 (GSAU-DKZY-2024-004))}, authors=null, keywords=[Keyword(id=1309574841083199675, tenantId=1146029695717560320, journalId=1308397196074385509, 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Carbon and water fluxes and their coupling in an Alpine meadow ecosystem on the northeastern Tibetan Plateau[J]. Theoretical and Applied Climatology, 2020, 142(1):1-18 DUAN Y Y, YANG H, LI J, et al. Effects of plateau pika disturbance on soil net nitrogen mineralization from small to large scales in Alpine meadows[J]. Catena, 2025, 259:109384 WEI L M, ZHAO W Z, FENG X Y, et al. Freeze-thaw desertification of Alpine meadow in Qilian Mountains and the implications for Alpine ecosystem management[J]. Catena, 2023, 232:107471 FAN J, LIANG B, LIU H C, et al. Impact on local sustainability of the northward expansion of human activities into protected areas in northern Tibet[J]. Land Degradation & Development, 2022, 33(15):2945-2959 ZHANG Y. Short-term impacts of tourism trampling on Shangri-la Alpine meadow vegetation[D]. Kunming:Yunnan University, 2013:1-2 张勇. 旅游踩踏对香格里拉高寒草甸植物群落的短期影响[D]. 昆明:云南大学, 2013:1-2 YU G, LU C X, XIE G D. Soil conservation capacity of Alpine meadow ecosystem and its economic value in the northern Qinghai-Tibetan Plateau[J]. Journal of Beijing Forestry University, 2006, 28(4):57-61 于格, 鲁春霞, 谢高地. 青藏高原北缘地区高寒草甸土壤保持功能及其价值的实验研究[J]. 北京林业大学学报, 2006, 28(4):57-61 BAO Z P, LIN D, LIU X P, et al. Effects of grazing intensities on soil organic carbon and its fractions in Alpine meadows[J]. Acta Agrestia Sinica, 2026, 34(1):172-181 包志鹏, 林栋, 刘雪鹏, 等. 放牧强度对高寒草甸土壤有机碳及其组分的影响[J]. 草地学报, 2026, 34(1):172-181 XU Z, LI X, ZHANG L. A bibliometric analysis of research trends and hotspots in Alpine grassland degradation on the Qinghai-Tibet Plateau[J]. PeerJ, 2023, 11:e16210 SHI M M, WANG Z, ZHOU B R, et al. Characteristics of grassland degradation and its relationship with climate factors on Qinghai-Tibetan Plateau, China[J]. Chinese Journal of Applied Ecology, 2022, 33(12):3271-3278 石明明, 王喆, 周秉荣, 等. 青藏高原草地退化特征及其与气候因子的关系[J]. 应用生态学报, 2022, 33(12):3271-3278 阿里地区林业和草原局. 第二轮中央生态环境保护督察反馈意见(27-02)整改措施完成情况公示表[EB/OL]. https://lycy.al.gov.cn/info/2363/36542.htm, 2025-11-04/2026-02-26 XIONG Q L, XIAO Y, LIANG P H, et al. Trends in climate change and human interventions indicate grassland productivity on the Qinghai-Tibetan Plateau from 1980 to 2015[J]. Ecological Indicators, 2021, 129:108010 CHEN X Y, WANG X L, SONG Y H, et al. A review of studies on the effects of anthropogenic disturbances on plant-soil-microorganism interactions in grassland ecosystems: based on grazing and tourism perspectives[J]. Agronomy, 2024, 14(12):2890 YANG S Q, HAN Y, WU Q H, et al. Field mulching techniques: evolution, advances, and future perspectives[J]. Journal of China Agricultural University, 2025, 30(9):15-26 杨世琦, 韩钰, 武清涵, 等. 农田覆盖技术的演进、发展与展望[J]. 中国农业大学学报, 2025, 30(9):15-26 ZHANG B G, ZHAO Y T, LIU X J, et al. Research on response characteristics of Alpine meadow microbial population to tillage in the upstream regions of Shule River, Qinghai-Tibet Plateau[J]. Journal of Glaciology and Geocryology, 2020, 42(3):1027-1035 张宝贵, 赵宇婷, 刘晓娇, 等. 翻耕补播对青藏高原疏勒河上游高寒草甸土壤可培养微生物数量的影响[J]. 冰川冻土, 2020, 42(3):1027-1035 MWAFULIRWA L, SIZMUR T, DAYMOND A, et al. Cocoa pod husk-derived organic soil amendments differentially affect soil fertility, nutrient leaching, and greenhouse gas emissions in cocoa soils[J]. Journal of Cleaner Production, 2024, 479:144065 HAUTIER Y, NIKLAUS P A, HECTOR A. Competition for light causes plant biodiversity loss after eutrophication[J]. Science, 2009, 324(5927):636-638 HAUTIER Y, SEABLOOM E W, BORER E T, et al. Eutrophication weakens stabilizing effects of diversity in natural grasslands[J]. Nature, 2014, 508(7497):521-525 CARROLL O, BATZER E, BHARATH S, et al. Nutrient identity modifies the destabilising effects of eutrophication in grasslands[J]. Ecology Letters, 2022, 25(4):754-765 KÖRSCHENS M, ALBERT E, ARMBRUSTER M, et al. Effect of mineral and organic fertilization on crop yield, nitrogen uptake, carbon and nitrogen balances, as well as soil organic carbon content and dynamics: results from 20 European long-term field experiments of the twenty-first century[J]. Archives of Agronomy and Soil Science, 2013, 59(8):1017-1040 ZHOU C F, DUN X J, TANG Q, et al. Elevation-dependent shifts in soil phosphorus forms and phosphorus-solubilizing microbial diversity suggest enhanced bioavailable phosphorus cycling with rising temperatures[J]. Microbiology Spectrum, 2025, 13(8):e01300-e01324 GAO Y X, SONG X, ZHENG W K, et al. The controlled-release nitrogen fertilizer driving the symbiosis of microbial communities to improve wheat productivity and soil fertility[J]. Field Crops Research, 2022, 289:108712 KOTAS P, CHOMA M, ŠANTRŮČKOVÁ H, et al. Linking above- and belowground responses to 16 years of fertilization, mowing, and removal of the dominant species in a temperate grassland[J]. Ecosystems, 2017, 20(2):354-367 ZHAO Y, ZHANG M L, LIU Z Q, et al. Migration and transformation of soil phosphorus by organic acids: a global meta-analysis[J]. Journal of Soils and Sediments, 2024, 24(2):589-602 LIU Y. Effects of multi-gradient nitrogen and phosphorus addition on community structure and productivity of Alpine meadow[D]. Xining:Qinghai University, 2023:11-46 刘艳. 多梯度氮、磷添加对高寒草甸群落结构和生产力的影响[D]. 西宁:青海大学, 2023:11-46 THEODOSE T A, BOWMAN W D. Nutrient availability, plant abundance, and species diversity in two Alpine tundra communities[J]. Ecology, 1997, 78(6):1861-1872 PEÑUELAS J, POULTER B, SARDANS J, et al. Human-induced nitrogen-phosphorus imbalances alter natural and managed ecosystems across the globe[J]. Nature Communications, 2013, 4:2934 JI G X, HU G Z, GAO Q Z, et al. N limitation may inhibit the effectiveness of close-to-nature restoration measures for degraded Alpine meadows on the northern Qinghai-Tibet Plateau[J]. Basic and Applied Ecology, 2024, 77:35-44 鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社, 2000: AITKEN R L, MOODY P W. The effect of valence and Ionic-strength on the measurement of pH buffer capacity[J]. Australian Journal of Soil Research, 1994, 32(5):975-984 YANG D J, HUANG Y, HU Z D. Preferable oxidation conditions for determination of soil organic matter by hot hydration method[J]. Guangxi Agricultural Sciences, 2009(10):1335-1338 杨代军, 黄莺, 虎振东. 水合热法测定土壤有机质含量的氧化条件优选[J]. 广西农业科学, 2009(10):1335-1338 LI S Q, LIU J J, GU Y L, et al. Evaluation of uncertainty in the determination of total phosphorus content in fructus aurantii by molybdenum antimony anticolorimetric method[J]. Modern Food, 2022(23):123-126 李诗琴, 刘俊俊, 谷云龙, 等. 钼锑抗比色法测定枳壳中全磷含量的不确定度评估[J]. 现代食品, 2022(23):123-126 CAO K H, ZHANG J L, LI S D. Comparison of two methods for determination of total phosphorus in soil[J]. Journal of Baoshan Teachers College, 2018, 37(5):26-29 曹凯红, 章金龙, 李树德. 两种测定土壤全磷含量方法对比[J]. 保山学院学报, 2018, 37(5):26-29 TIAN M, AN H, LIU Z J, et al. The response characteristics of grassland species diversity and productivity to environmental factors in the three-river-source area[J]. Acta Agrestia Sinica, 2025, 33(12):3983-3993 田淼, 安航, 刘智军, 等. 三江源区草地物种多样性和生产力对环境因子的响应特征[J]. 草地学报, 2025, 33(12):3983-3993 MA J J, LI X L, YIN T J, et al. Study on succession characteristics of plant communities and soil limiting factors in degraded patches of alpine meadow[J/OL]. https://link.cnki.net/urlid/11.3362.S.20260104.1549.002, 2026-01-04/2026-02-26 马婧娟, 李希来, 尹通江, 等.高寒草甸退化斑块植物群落演替特征及土壤限制因子研究[J/OL]..https://link.cnki.net/urlid/11.3362.S.20260104.1549.002, 2026-01-04/2026-02-26 WU M Q, PHALEMPIN M, REITZ T, et al. Links between soil microstructure dynamics and carbon cycling in response to land use and climate change[J]. Soil Biology and Biochemistry, 2025, 211:109982 DUAN Y F, XIE B H, LV W G, et al. Earthworm-biochar linkage drives saline-alkali soil health restoration: processes, mechanisms and prospects[J]. Desalination, 2026, 620:119603 CHICHONGUE O, VAN TOL J, CERONIO G, et al. Effects of tillage systems and cropping patterns on soil physical properties in Mozambique[J]. Agriculture, 2020, 10(10):448 ZHOU J, SUN T T, SHI L L, et al. Organic carbon accumulation and microbial activities in arable soils after abandonment: A chronosequence study[J]. Geoderma, 2023, 435:116496 RAIESI F, SALEK-GILANI S. The potential activity of soil extracellular enzymes as an indicator for ecological restoration of rangeland soils after agricultural abandonment[J]. Applied Soil Ecology, 2018, 126:140-147 YAN G Y, LUO X, HUANG B B, et al. Imbalance in nitrogen and phosphorus allocation between tree roots and leaves induced by nitrogen addition[J]. Science of the Total Environment, 2025, 958:177925 WANG S Q, SONG M H, WANG C M, et al. Mechanisms underlying soil microbial regulation of available phosphorus in a temperate forest exposed to long-term nitrogen addition[J]. Science of the Total Environment, 2023, 904:166403 LUO M, MOORHEAD D L, OCHOA-HUESO R, et al. Nitrogen loading enhances phosphorus limitation in terrestrial ecosystems with implications for soil carbon cycling[J]. Functional Ecology, 2022, 36(11):2845-2858 ZHANG J Q, LIU Y H, DONG J Y. Responses of Alpine soil nitrification and denitrification rates to nitrogen addition gradient—the role of functional genes[J]. Agriculture, 2023, 13(2):245 LI J H, YANG Y J, LI B W, et al. Effects of nitrogen and phosphorus fertilization on soil carbon fractions in Alpine meadows on the Qinghai-Tibetan Plateau[J]. Plos One, 2014, 9(7):e103266 SPOHN M, SCHLEUSS P M. Addition of inorganic phosphorus to soil leads to desorption of organic compounds and thus to increased soil respiration[J]. Soil Biology and Biochemistry, 2019, 130:220-226 QIN W K, FENG J G, ZHANG Q F, et al. Nitrogen and phosphorus addition mediate soil priming effects via affecting microbial stoichiometric balance in an Alpine meadow[J]. Science of the Total Environment, 2024, 908:168350 SUN B, ROBERTS D M, DENNIS P G, et al. Microbial properties and nitrogen contents of arable soils under different tillage regimes[J]. Soil Use and Management, 2014, 30(1):152-159 TAO J J, FAN L C, JIANG T, et al. In situ visualization of soil profile acidification and processes following nitrogen fertilization and liming[J]. Geoderma, 2025, 456:117243 BORER E T, SEABLOOM E W, GRUNER D S, et al. Herbivores and nutrients control grassland plant diversity via light limitation[J]. Nature, 2014, 508(7497):517-520 ZHANG P F, HEFTING M M, SOONS M B, et al. Fast and furious: early differences in growth rate drive short-term plant dominance and exclusion under eutrophication[J]. Ecology and Evolution, 2020, 10(18):10116-10129 DYBZINSKI R, TILMAN D. Resource use patterns predict long-term outcomes of plant competition for nutrients and light[J]. The American Naturalist, 2007, 170(3):305-318 ZONG N, SHI P L. Enhanced community production rather than structure improvement under nitrogen and phosphorus addition in severely degraded Alpine meadows[J]. Sustainability, 2019, 11(7):2023 DOLEŽAL J, LANTA V, MUDRÁK O, et al. Seasonality promotes grassland diversity: interactions with mowing, fertilization and removal of dominant species[J]. Journal of Ecology, 2019, 107(1):203-215 GAO T M, ZHANG R Q, LIU T J, et al. Effect of Shallow tillage during different season on grassland vegetation and soil in the Xilamuren region of North Yin Mountains[J]. Pratacultural Science, 2012, 29(5):676-680 高天明, 张瑞强, 刘铁军, 等. 阴山北麓浅耕翻季节对草地植被和土壤的影响[J]. 草业科学, 2012, 29(5):676-680 ZHAO W H, JU C Y, XIAO Q L, et al. Effect of tillage depths on root growth, biomass accumulation and quality of tobacco plants[J]. Chinese Journal of Soil Science, 2025, 56(5):1288-1296 赵伟浩, 琚晨仪, 肖庆礼, 等. 翻耕深度对烤烟根系发育和生物量积累及质量的影响[J]. 土壤通报, 2025, 56(5):1288-1296 WANG H, WANG Q J, SHAO M A, et al. Influence of tillage and compaction on solute transport process on the sloping land[J]. Science of Soil and Water Conservation, 2008, 6(6):21-25, 86 王辉, 王全九, 邵明安, 等. 翻耕与压实对坡地土壤溶质迁移过程的影响[J]. 中国水土保持科学, 2008, 6(6):21-25, 86 GAO Z C, TIAN J N, HUO Y S, et al. Effects of root pruning and shallow plowing on soil properties and plant communities in deteriorated steppe[J]. Acta Ecologica Sinica, 2017, 37(11):3824-3829 高志成, 田佳妮, 霍艳双, 等. 切根和浅耕翻措施对退化草地生长季土壤性质及植物群落的影响[J]. 生态学报, 2017, 37(11):3824-3829 JARNE A, USÓN A, REINÉ R. Management practices in mountain meadows: consequences for soil nutrient availability[J]. Agronomy, 2024, 14(10):2419)
Plowing and fertilization are common management practices in alpine meadows, which directly influence plant productivity and species diversity. This study established experimental plots with plowing (Dis), nutrient addition (NPK), and their interactive treatment (DisNPK) at the Tianzhu Alpine Grassland Ecosystem Experimental Station of Gansu Agricultural University, with natural grassland as a control (CK). The objectives were to investigate the effects of plowing and nutrient addition on plant community structure and biomass in alpine meadows and to identify the key driving factors. By measuring soil physicochemical properties, plant biomass, and species diversity, we analyzed the relationships between soil physicochemical properties and vegetation biomass/species diversity. The results showed that all treatments significantly increased the contents of total and available soil nutrients compared to control. The NPK treatment significantly increased aboveground biomass, and the combined effect of plowing and fertilization (DisNPK) had the most significant enhancement on productivity (particularly for grasses and above-ground biomass). The Dis and DisNPK treatments significantly reduced community diversity. Correlation analysis revealed that soil available nutrients and organic matter were positively correlated with above-ground biomass but negatively correlated with diversity indices (P<0.05). This study demonstrates that plowing and nutrient addition can alter the community structure and productivity of the ecosystem, providing a theoretical basis for the ecological restoration of alpine meadows.
WANG Y Y, MA Y M, LI H X, et al. Carbon and water fluxes and their coupling in an Alpine meadow ecosystem on the northeastern Tibetan Plateau[J]. Theoretical and Applied Climatology, 2020, 142(1):1-18 DUAN Y Y, YANG H, LI J, et al. Effects of plateau pika disturbance on soil net nitrogen mineralization from small to large scales in Alpine meadows[J]. Catena, 2025, 259:109384 WEI L M, ZHAO W Z, FENG X Y, et al. Freeze-thaw desertification of Alpine meadow in Qilian Mountains and the implications for Alpine ecosystem management[J]. Catena, 2023, 232:107471 FAN J, LIANG B, LIU H C, et al. Impact on local sustainability of the northward expansion of human activities into protected areas in northern Tibet[J]. Land Degradation & Development, 2022, 33(15):2945-2959 ZHANG Y. Short-term impacts of tourism trampling on Shangri-la Alpine meadow vegetation[D]. Kunming:Yunnan University, 2013:1-2 张勇. 旅游踩踏对香格里拉高寒草甸植物群落的短期影响[D]. 昆明:云南大学, 2013:1-2 YU G, LU C X, XIE G D. Soil conservation capacity of Alpine meadow ecosystem and its economic value in the northern Qinghai-Tibetan Plateau[J]. Journal of Beijing Forestry University, 2006, 28(4):57-61 于格, 鲁春霞, 谢高地. 青藏高原北缘地区高寒草甸土壤保持功能及其价值的实验研究[J]. 北京林业大学学报, 2006, 28(4):57-61 BAO Z P, LIN D, LIU X P, et al. Effects of grazing intensities on soil organic carbon and its fractions in Alpine meadows[J]. Acta Agrestia Sinica, 2026, 34(1):172-181 包志鹏, 林栋, 刘雪鹏, 等. 放牧强度对高寒草甸土壤有机碳及其组分的影响[J]. 草地学报, 2026, 34(1):172-181 XU Z, LI X, ZHANG L. A bibliometric analysis of research trends and hotspots in Alpine grassland degradation on the Qinghai-Tibet Plateau[J]. PeerJ, 2023, 11:e16210 SHI M M, WANG Z, ZHOU B R, et al. Characteristics of grassland degradation and its relationship with climate factors on Qinghai-Tibetan Plateau, China[J]. Chinese Journal of Applied Ecology, 2022, 33(12):3271-3278 石明明, 王喆, 周秉荣, 等. 青藏高原草地退化特征及其与气候因子的关系[J]. 应用生态学报, 2022, 33(12):3271-3278 阿里地区林业和草原局. 第二轮中央生态环境保护督察反馈意见(27-02)整改措施完成情况公示表[EB/OL]. https://lycy.al.gov.cn/info/2363/36542.htm, 2025-11-04/2026-02-26 XIONG Q L, XIAO Y, LIANG P H, et al. Trends in climate change and human interventions indicate grassland productivity on the Qinghai-Tibetan Plateau from 1980 to 2015[J]. Ecological Indicators, 2021, 129:108010 CHEN X Y, WANG X L, SONG Y H, et al. A review of studies on the effects of anthropogenic disturbances on plant-soil-microorganism interactions in grassland ecosystems: based on grazing and tourism perspectives[J]. Agronomy, 2024, 14(12):2890 YANG S Q, HAN Y, WU Q H, et al. Field mulching techniques: evolution, advances, and future perspectives[J]. Journal of China Agricultural University, 2025, 30(9):15-26 杨世琦, 韩钰, 武清涵, 等. 农田覆盖技术的演进、发展与展望[J]. 中国农业大学学报, 2025, 30(9):15-26 ZHANG B G, ZHAO Y T, LIU X J, et al. Research on response characteristics of Alpine meadow microbial population to tillage in the upstream regions of Shule River, Qinghai-Tibet Plateau[J]. Journal of Glaciology and Geocryology, 2020, 42(3):1027-1035 张宝贵, 赵宇婷, 刘晓娇, 等. 翻耕补播对青藏高原疏勒河上游高寒草甸土壤可培养微生物数量的影响[J]. 冰川冻土, 2020, 42(3):1027-1035 MWAFULIRWA L, SIZMUR T, DAYMOND A, et al. Cocoa pod husk-derived organic soil amendments differentially affect soil fertility, nutrient leaching, and greenhouse gas emissions in cocoa soils[J]. Journal of Cleaner Production, 2024, 479:144065 HAUTIER Y, NIKLAUS P A, HECTOR A. Competition for light causes plant biodiversity loss after eutrophication[J]. Science, 2009, 324(5927):636-638 HAUTIER Y, SEABLOOM E W, BORER E T, et al. Eutrophication weakens stabilizing effects of diversity in natural grasslands[J]. Nature, 2014, 508(7497):521-525 CARROLL O, BATZER E, BHARATH S, et al. Nutrient identity modifies the destabilising effects of eutrophication in grasslands[J]. Ecology Letters, 2022, 25(4):754-765 KÖRSCHENS M, ALBERT E, ARMBRUSTER M, et al. Effect of mineral and organic fertilization on crop yield, nitrogen uptake, carbon and nitrogen balances, as well as soil organic carbon content and dynamics: results from 20 European long-term field experiments of the twenty-first century[J]. Archives of Agronomy and Soil Science, 2013, 59(8):1017-1040 ZHOU C F, DUN X J, TANG Q, et al. Elevation-dependent shifts in soil phosphorus forms and phosphorus-solubilizing microbial diversity suggest enhanced bioavailable phosphorus cycling with rising temperatures[J]. Microbiology Spectrum, 2025, 13(8):e01300-e01324 GAO Y X, SONG X, ZHENG W K, et al. The controlled-release nitrogen fertilizer driving the symbiosis of microbial communities to improve wheat productivity and soil fertility[J]. Field Crops Research, 2022, 289:108712 KOTAS P, CHOMA M, ŠANTRŮČKOVÁ H, et al. Linking above- and belowground responses to 16 years of fertilization, mowing, and removal of the dominant species in a temperate grassland[J]. Ecosystems, 2017, 20(2):354-367 ZHAO Y, ZHANG M L, LIU Z Q, et al. Migration and transformation of soil phosphorus by organic acids: a global meta-analysis[J]. Journal of Soils and Sediments, 2024, 24(2):589-602 LIU Y. Effects of multi-gradient nitrogen and phosphorus addition on community structure and productivity of Alpine meadow[D]. Xining:Qinghai University, 2023:11-46 刘艳. 多梯度氮、磷添加对高寒草甸群落结构和生产力的影响[D]. 西宁:青海大学, 2023:11-46 THEODOSE T A, BOWMAN W D. Nutrient availability, plant abundance, and species diversity in two Alpine tundra communities[J]. Ecology, 1997, 78(6):1861-1872 PEÑUELAS J, POULTER B, SARDANS J, et al. Human-induced nitrogen-phosphorus imbalances alter natural and managed ecosystems across the globe[J]. Nature Communications, 2013, 4:2934 JI G X, HU G Z, GAO Q Z, et al. N limitation may inhibit the effectiveness of close-to-nature restoration measures for degraded Alpine meadows on the northern Qinghai-Tibet Plateau[J]. Basic and Applied Ecology, 2024, 77:35-44 鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社, 2000: AITKEN R L, MOODY P W. The effect of valence and Ionic-strength on the measurement of pH buffer capacity[J]. Australian Journal of Soil Research, 1994, 32(5):975-984 YANG D J, HUANG Y, HU Z D. Preferable oxidation conditions for determination of soil organic matter by hot hydration method[J]. Guangxi Agricultural Sciences, 2009(10):1335-1338 杨代军, 黄莺, 虎振东. 水合热法测定土壤有机质含量的氧化条件优选[J]. 广西农业科学, 2009(10):1335-1338 LI S Q, LIU J J, GU Y L, et al. Evaluation of uncertainty in the determination of total phosphorus content in fructus aurantii by molybdenum antimony anticolorimetric method[J]. Modern Food, 2022(23):123-126 李诗琴, 刘俊俊, 谷云龙, 等. 钼锑抗比色法测定枳壳中全磷含量的不确定度评估[J]. 现代食品, 2022(23):123-126 CAO K H, ZHANG J L, LI S D. Comparison of two methods for determination of total phosphorus in soil[J]. Journal of Baoshan Teachers College, 2018, 37(5):26-29 曹凯红, 章金龙, 李树德. 两种测定土壤全磷含量方法对比[J]. 保山学院学报, 2018, 37(5):26-29 TIAN M, AN H, LIU Z J, et al. The response characteristics of grassland species diversity and productivity to environmental factors in the three-river-source area[J]. Acta Agrestia Sinica, 2025, 33(12):3983-3993 田淼, 安航, 刘智军, 等. 三江源区草地物种多样性和生产力对环境因子的响应特征[J]. 草地学报, 2025, 33(12):3983-3993 MA J J, LI X L, YIN T J, et al. Study on succession characteristics of plant communities and soil limiting factors in degraded patches of alpine meadow[J/OL]. https://link.cnki.net/urlid/11.3362.S.20260104.1549.002, 2026-01-04/2026-02-26 马婧娟, 李希来, 尹通江, 等.高寒草甸退化斑块植物群落演替特征及土壤限制因子研究[J/OL]..https://link.cnki.net/urlid/11.3362.S.20260104.1549.002, 2026-01-04/2026-02-26 WU M Q, PHALEMPIN M, REITZ T, et al. Links between soil microstructure dynamics and carbon cycling in response to land use and climate change[J]. Soil Biology and Biochemistry, 2025, 211:109982 DUAN Y F, XIE B H, LV W G, et al. Earthworm-biochar linkage drives saline-alkali soil health restoration: processes, mechanisms and prospects[J]. Desalination, 2026, 620:119603 CHICHONGUE O, VAN TOL J, CERONIO G, et al. Effects of tillage systems and cropping patterns on soil physical properties in Mozambique[J]. Agriculture, 2020, 10(10):448 ZHOU J, SUN T T, SHI L L, et al. Organic carbon accumulation and microbial activities in arable soils after abandonment: A chronosequence study[J]. Geoderma, 2023, 435:116496 RAIESI F, SALEK-GILANI S. The potential activity of soil extracellular enzymes as an indicator for ecological restoration of rangeland soils after agricultural abandonment[J]. Applied Soil Ecology, 2018, 126:140-147 YAN G Y, LUO X, HUANG B B, et al. Imbalance in nitrogen and phosphorus allocation between tree roots and leaves induced by nitrogen addition[J]. Science of the Total Environment, 2025, 958:177925 WANG S Q, SONG M H, WANG C M, et al. Mechanisms underlying soil microbial regulation of available phosphorus in a temperate forest exposed to long-term nitrogen addition[J]. Science of the Total Environment, 2023, 904:166403 LUO M, MOORHEAD D L, OCHOA-HUESO R, et al. Nitrogen loading enhances phosphorus limitation in terrestrial ecosystems with implications for soil carbon cycling[J]. Functional Ecology, 2022, 36(11):2845-2858 ZHANG J Q, LIU Y H, DONG J Y. Responses of Alpine soil nitrification and denitrification rates to nitrogen addition gradient—the role of functional genes[J]. Agriculture, 2023, 13(2):245 LI J H, YANG Y J, LI B W, et al. Effects of nitrogen and phosphorus fertilization on soil carbon fractions in Alpine meadows on the Qinghai-Tibetan Plateau[J]. Plos One, 2014, 9(7):e103266 SPOHN M, SCHLEUSS P M. Addition of inorganic phosphorus to soil leads to desorption of organic compounds and thus to increased soil respiration[J]. Soil Biology and Biochemistry, 2019, 130:220-226 QIN W K, FENG J G, ZHANG Q F, et al. Nitrogen and phosphorus addition mediate soil priming effects via affecting microbial stoichiometric balance in an Alpine meadow[J]. Science of the Total Environment, 2024, 908:168350 SUN B, ROBERTS D M, DENNIS P G, et al. Microbial properties and nitrogen contents of arable soils under different tillage regimes[J]. Soil Use and Management, 2014, 30(1):152-159 TAO J J, FAN L C, JIANG T, et al. In situ visualization of soil profile acidification and processes following nitrogen fertilization and liming[J]. Geoderma, 2025, 456:117243 BORER E T, SEABLOOM E W, GRUNER D S, et al. Herbivores and nutrients control grassland plant diversity via light limitation[J]. Nature, 2014, 508(7497):517-520 ZHANG P F, HEFTING M M, SOONS M B, et al. Fast and furious: early differences in growth rate drive short-term plant dominance and exclusion under eutrophication[J]. Ecology and Evolution, 2020, 10(18):10116-10129 DYBZINSKI R, TILMAN D. Resource use patterns predict long-term outcomes of plant competition for nutrients and light[J]. The American Naturalist, 2007, 170(3):305-318 ZONG N, SHI P L. Enhanced community production rather than structure improvement under nitrogen and phosphorus addition in severely degraded Alpine meadows[J]. Sustainability, 2019, 11(7):2023 DOLEŽAL J, LANTA V, MUDRÁK O, et al. Seasonality promotes grassland diversity: interactions with mowing, fertilization and removal of dominant species[J]. Journal of Ecology, 2019, 107(1):203-215 GAO T M, ZHANG R Q, LIU T J, et al. Effect of Shallow tillage during different season on grassland vegetation and soil in the Xilamuren region of North Yin Mountains[J]. Pratacultural Science, 2012, 29(5):676-680 高天明, 张瑞强, 刘铁军, 等. 阴山北麓浅耕翻季节对草地植被和土壤的影响[J]. 草业科学, 2012, 29(5):676-680 ZHAO W H, JU C Y, XIAO Q L, et al. Effect of tillage depths on root growth, biomass accumulation and quality of tobacco plants[J]. Chinese Journal of Soil Science, 2025, 56(5):1288-1296 赵伟浩, 琚晨仪, 肖庆礼, 等. 翻耕深度对烤烟根系发育和生物量积累及质量的影响[J]. 土壤通报, 2025, 56(5):1288-1296 WANG H, WANG Q J, SHAO M A, et al. Influence of tillage and compaction on solute transport process on the sloping land[J]. Science of Soil and Water Conservation, 2008, 6(6):21-25, 86 王辉, 王全九, 邵明安, 等. 翻耕与压实对坡地土壤溶质迁移过程的影响[J]. 中国水土保持科学, 2008, 6(6):21-25, 86 GAO Z C, TIAN J N, HUO Y S, et al. Effects of root pruning and shallow plowing on soil properties and plant communities in deteriorated steppe[J]. Acta Ecologica Sinica, 2017, 37(11):3824-3829 高志成, 田佳妮, 霍艳双, 等. 切根和浅耕翻措施对退化草地生长季土壤性质及植物群落的影响[J]. 生态学报, 2017, 37(11):3824-3829 JARNE A, USÓN A, REINÉ R. Management practices in mountain meadows: consequences for soil nutrient availability[J]. Agronomy, 2024, 14(10):2419