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The results showed that both entomopathogenic fungi provided effective control of Odontothrips loti, with average control efficacies of 34.8% and 30.3%, respectively. Compared with the control, three years of B. bassiana application significantly improved soil physicochemical properties, with available phosphorus and soil organic carbon increasing by 67.2% and 41.7%, respectively, showing greater improvement than M. anisopliae. Soil enzyme activities were also enhanced, with increases of 36.91% in sucrase, 64.20% in alkaline phosphatase, and 31.03% in catalase. Application of M. anisopliae increased sucrase activity by 30.32% and catalase activity by 24.42% relative to the control. Compared with the soil bacterial community, soil fungal community structure was more strongly altered by the two biocontrol fungal treatments. Meanwhile, the relative abundance of beneficial taxa such as Sphingomonas increased, whereas the proportion of pathogenic fungi such as Fusarium declined. Overall, this study provides a theoretical basis and practical reference for pest biological control and the sustainable development of grassland agriculture in the Ningxia region., authors=WANG Yu-zhe1,2, CUI Jun-ling3, WANG Jia-rui1,2, LI Zhi-qiu1,2, WANG Ying1, SUN Wei1, ZHANG Rong1, BAN Li-ping2, WEI Shu-hua1, authorsList=WANG Yu-zhe, CUI Jun-ling, WANG Jia-rui, LI Zhi-qiu, WANG Ying, SUN Wei, ZHANG Rong, BAN Li-ping, WEI Shu-hua, authorCompany=1 Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, Ningxia 750002, China; 2 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China; 3 Yinchuan Park Management Center, Yinchuan, Ningxia 750000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574771491303616, articleId=1309574771269005503, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=两种生防菌对苜蓿主要害虫的防效及土壤根际微生态的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=本研究在宁夏农林科学院植物保护研究所芦花台试验基地苜蓿田,间施3年球孢白僵菌Beauveria bassiana及金龟子绿僵菌Metarhizium anisopliae,发现两种生防菌对牛角花齿蓟马具有较高防效,防治效果平均分别为34.8%和30.3%。与对照组相比,间施3年球孢白僵菌后,土壤理化性质得到显著改善,速效磷、有机碳分别增加了67.2%,41.7%,改善效果超过金龟子绿僵菌;土壤酶活性提高,蔗糖酶、碱性磷酸酶、过氧化氢酶分别增加了36.91%,64.20%,31.03%。金龟子绿僵菌使蔗糖酶活性较对照增加了30.32%、过氧化氢酶活性增加了24.42%。与土壤细菌相比,两种生防菌处理组对土壤真菌结构改变较大,部分有益菌如鞘氨醇单胞菌丰度增加,部分病原菌如镰刀菌占比下降。本研究为害虫生物防治及宁夏地区草地农业可持续发展提供了理论依据和实践参考。, authors=王誉喆1,2, 崔竣岭3, 王嘉瑞1,2, 李知秋1,2, 王颖1, 孙伟1, 张蓉1, 班丽萍2, 魏淑花1, authorsList=王誉喆, 崔竣岭, 王嘉瑞, 李知秋, 王颖, 孙伟, 张蓉, 班丽萍, 魏淑花, authorCompany=1 宁夏农林科学院植物保护研究所, 宁夏 银川 750002; 2 中国农业大学草业科学与技术学院, 北京 100193; 3 银川市公园管理中心, 宁夏 银川 750000, correspAuthors=null, authorNote=王誉喆(2000-),女,汉族,河南信阳人,博士研究生,主要从事草地植物保护方向研究,E-mail:13782910269@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=/DouW/97rj0K7EMNIElNBQ==, pdfFileSize=3053984, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=宁夏自然科学基金项目 (2024AAC02065); 宁夏农业高质量发展和生态保护科技创新示范课题 (NGSB-2021-15-04); 国家牧草产业技术体系-盐池综合试验站 (CARS-34); 国家自然科学基金项目 (32573603))}, authors=null, keywords=[Keyword(id=1309574840915419275, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574771269005503, language=CN, orderNo=1, keyword=苜蓿害虫), Keyword(id=1309574841129328780, 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pdfUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/PDF/10.11733/j.issn.1007-0435.2026.07.008, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1784044800000, fullTextJson=null, articleText=null, reference=ZHANG D, LONG H Y. Evaluation of production performance and nutritional value of eight alfalfa varieties in the hot-arid zone[J]. Chinese Journal of Grassland, 2024, 46(1):70-77 张德, 龙会英. 8个紫花苜蓿品种在干热区生产性能和营养价值评价[J]. 中国草地学报, 2024, 46(1):70-77 YUE Y H, QI X, WANG Y R, et al. Persistence of 35 Medicago sativa varieties at the 10th year after establishment[J]. Acta Prataculturae Sinica, 2014, 23(1):58-64 岳彦红, 齐晓, 王彦荣, 等. 35个10龄紫花苜蓿品种的持久性比较[J]. 草业学报, 2014, 23(1):58-64 CHU J X, ZHANG L Z. The quality characteristics and application of alfalfa in animal production[J]. China Feed, 2022(11):91-95 初建香, 张立中. 紫花苜蓿的品质特性及其在动物生产中的应用[J]. 中国饲料, 2022(11):91-95 WANG Q B, ZOU Y. China’s alfalfa market and imports: development, trends, and potential impacts of the U.S.-China trade dispute and retaliations[J]. Journal of Integrative Agriculture, 2020, 19(4):1149-1158 SUN Q Z, YU Z, XU C C. Urgency of further developing alfalfa industry in China[J]. Pratacultural Science, 2012(2):314-319 孙启忠, 玉柱, 徐春城. 我国苜蓿产业亟待振兴[J]. 草业科学, 2012(2):314-319 RODRÍGUEZ D, COY-BARRERA E. Overview of updated control tactics for western flower Thrips[J]. Insects, 2023, 14(7):649 LI N, MA X X, WANG X P. Research progress on a major insect pest of Alfalfa Odontothrips loti[J]. China Plant Protection, 2019, 39(11):31-35 李楠, 马晓霞, 王新谱. 苜蓿重要害虫牛角花齿蓟马研究进展[J]. 中国植保导刊, 2019, 39(11):31-35 QIAO L, ZHANG Z Q, BAN L P, et al. Optimization trapping technology for controlling Odontothrips loti(Haliday)(Thysanoptera, Thripidae) in alfalfa field[J]. Chinese Journal of Biological Control, 2023, 39(6):1351-1360 乔浪, 张志强, 班丽萍, 等. 紫花苜蓿田牛角花齿蓟马诱集技术优化研究[J]. 中国生物防治学报, 2023, 39(6):1351-1360 TANG L D, YAN K L, FU B L, et al. The life table parameters of Megalurothrips usitatus (Thysanoptera: Thripidae) on four leguminous crops[J]. The Florida Entomologist, 2015, 98(2):620-625 SRINIVASAN R, PAOLA S, LIN M Y, et al. Development and validation of an integrated pest management strategy for the control of major insect pests on yard-long bean in Cambodia[J]. Crop Protection, 2019, 116:82-91 FU Z Y, LUO Y N, MIAO M, et al. Predation of Orius similis on different instars of the spotted alfalfa aphid in alfalfa[J]. Chinese Journal of Biological Control:1-11. 傅祖英, 罗撄宁, 苗淼, 等. 南方小花蝽对苜蓿斑蚜不同龄期的捕食作用 [J]. 中国生物防治学报: 1-11. CUI X N, XI B X, PENG B, et al. Predatory functional response of Orius minutus to Acyrthosiphon pisum and Odontothrips loti at different temperatures[J]. Chinese Journal of Biological Control, 2025, 41(2):276-283 崔晓宁, 席驳鑫, 彭斌, 等. 不同温度下微小花蝽对豌豆蚜和牛角花齿蓟马的捕食作用[J]. 中国生物防治学报, 2025, 41(2):276-283 WANG P, LIU X G, HUANG Z Y. Laboratory bioassay of five insecticides on against field dominant populations of Thrips[J]. Agrochemicals, 2016, 55(7):527-529 王彭, 刘叙杆, 黄正谊. 5种药剂对不同地区蓟马田间优势种群的敏感度测定[J]. 农药, 2016, 55(7):527-529 YE H C, WANG Q, YAN C, et al. The field efficacy and joint toxicity of emamectin benzoate and acetamiprid against cowpea Thrips[J]. Chinese Journal of Tropical Agriculture, 2020, 40(2):76-81 叶火春, 王琴, 闫超, 等. 甲维盐与啶虫脒对豇豆蓟马的联合毒力及田间防效[J]. 热带农业科学, 2020, 40(2):76-81 LIU K, TANG L D, LI P, et al. Toxocity and synergistic effect of the complex formulation of several insecticides to Megalurothrips usitatus (bagnall)[J]. Chinese Journal of Tropical Crops, 2014, 35(8):1615-1618 刘奎, 唐良德, 李鹏, 等. 几种杀虫剂对豆大蓟马的毒力测定及复配增效作用[J]. 热带作物学报, 2014, 35(8):1615-1618 LUO Y N, HAN C, TU X B, et al. Evaluation of trap systems for monitoring of Odontothrips loti and Frankliniella occidentalis: a pilot field trial[J]. Insects, 2026, 17(1):84 YAN S, REN X Y, WANG D J, et al. The research progress on the effects of entomopathogenic fungi on natural enemies[J]. Chinese Journal of Biological Control, 2023, 39(1):221-230 严森, 任小云, 王登杰, 等. 昆虫病原真菌在害虫防治中对天敌生物的影响研究进展[J]. 中国生物防治学报, 2023, 39(1):221-230 LI J, XIA Y X. Host-pathogen interactions between Metarhizium spp. and locusts[J]. Journal of Fungi, 2022, 8(6):602 PENG G X, ZHANG S L, XIA Y X. Metarhizium anisopliae CQMa421 and its application status[J]. Chinese Journal of Biological Control, 2020, 36(6):850-857 彭国雄, 张淑玲, 夏玉先. 金龟子绿僵菌CQMa421农药及应用情况[J]. 中国生物防治学报, 2020, 36(6):850-857 WU S Y, HE Z, WANG E D, et al. Application of Beauveria bassiana and Neoseiulus barkeri for improved control of Frankliniella occidentalis in greenhouse cucumber[J]. Crop Protection, 2017, 96:83-87 TADESSE MAWCHA K, MALINGA L, MUIR D, et al. Recent advances in biopesticide research and development with a focus on microbials[J]. F1000Research, 2025, 13:1071 WANG X W, JIANG X D, DING X H, et al. Biological characteristics and pathogenicity of four dominant pathogenic fungi of overwintering larvae of Ostrinia furnacalis in Xinjiang Desert oasis region[J]. Chinese Journal of Biological Control, 2023, 39(2):305-312 王小武, 蒋旭东, 丁新华, 等. 新疆荒漠绿洲区亚洲玉米螟越冬幼虫4种主要病原真菌生物学特性及致病力测定[J]. 中国生物防治学报, 2023, 39(2):305-312 CHI X X, CAO W, WANG C, et al. Effects of combined restoration measures on soil enzyme activities and microbial communities in degraded meadow steppe[J]. Acta Agrestia Sinica, 2026, 34(4): 1403-1412 迟晓雪, 曹薇, 王畅, 等. 复合改良措施对退化草甸草原土壤酶活性与微生物群落的影响[J]. 草地学报, 2026, 34(4): 1403-1412 TAN H Y, CHEN B R, YAN R R, et al. Research progress on grassland soil microbial characteristics and their responses to anthropogenic disturbance[J]. Acta Agrestia Sinica, 2014, 22(6): 1163-1170 谭红妍, 陈宝瑞, 闫瑞瑞, 等. 草地土壤微生物特性及其对人为干扰响应的研究进展[J]. 草地学报, 2014, 22(6): 1163-1170 JABER L R, OWNLEY B H. Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens?[J]. Biological Control, 2018, 116:36-45 BEHIE S W, MOREIRA C C, SEMENTCHOUKOVA I, et al. Carbon translocation from a plant to an insect-pathogenic endophytic fungus[J]. Nature Communications, 2017, 8:14245 WEI Q Y, LI Y Y, XU C, et al. Endophytic colonization by Beauveria bassiana increases the resistance of tomatoes against Bemisia tabaci[J]. Arthropod-Plant Interactions, 2020, 14(3):289-300 ZHANG Q K, WANG D J, LIU S, et al. Reproductive disruption induced by Beauveria bassiana in Frankliniellaoccidentalis as a potential long-term biocontrol strategy[J]. Pesticide Biochemistry and Physiology, 2025, 212:106464 MIR Y H, AHMAD GANIE M, SHAH T I, et al. Soil microbial and enzyme activities in different land use systems of the Northwestern Himalayas[J]. PeerJ, 2023, 11:e15993 BARELLI L, WALLER A S, BEHIE S W, et al. Plant microbiome analysis after Metarhizium amendment reveals increases in abundance of plant growth-promoting organisms and maintenance of disease-suppressive soil[J]. Plos One, 2020, 15(4):e0231150 PAVA-RIPOLL M, ANGELINI C, FANG W G, et al. The rhizosphere-competent entomopathogen Metarhizium anisopliae expresses a specific subset of genes in plant root exudate[J]. Microbiology, 2011, 157(1):47-55 DU W, ZHOU J, JIANG P, et al. Effects of Beauveria bassiana and acephate on enzyme activities and microbial diversity in paddy soil[J]. Plant, Soil and Environment, 2013, 59(12):562-567 ZAHN D K, HAVILAND D R, STANGHELLINI M E, et al. Evaluation of Beauveria bassiana for management of Citrus thrips (Thysanoptera: Thripidae) in California blueberries[J]. Journal of Economic Entomology, 2013, 106(5):1986-1995 BRUCK D J. Ecology of Metarhizium anisopliae in soilless potting media and the rhizosphere: implications for pest management[J]. Biological Control, 2005, 32(1):155-163)
Effects of Two Biocontrol Fungi on Major Alfalfa Pests and Rhizosphere Microecology
WANG Yu-zhe1,2, CUI Jun-ling3, WANG Jia-rui1,2, LI Zhi-qiu1,2, WANG Ying1, SUN Wei1, ZHANG Rong1, BAN Li-ping2, WEI Shu-hua1
Affiliations
1 Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, Ningxia 750002, China; 2 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China; 3 Yinchuan Park Management Center, Yinchuan, Ningxia 750000, China
This study was conducted in an alfalfa field at the Luhuatai experimental site of the Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, where Beauveria bassiana and Metarhizium anisopliae were applied in alternate rows for three consecutive years. The results showed that both entomopathogenic fungi provided effective control of Odontothrips loti, with average control efficacies of 34.8% and 30.3%, respectively. Compared with the control, three years of B. bassiana application significantly improved soil physicochemical properties, with available phosphorus and soil organic carbon increasing by 67.2% and 41.7%, respectively, showing greater improvement than M. anisopliae. Soil enzyme activities were also enhanced, with increases of 36.91% in sucrase, 64.20% in alkaline phosphatase, and 31.03% in catalase. Application of M. anisopliae increased sucrase activity by 30.32% and catalase activity by 24.42% relative to the control. Compared with the soil bacterial community, soil fungal community structure was more strongly altered by the two biocontrol fungal treatments. Meanwhile, the relative abundance of beneficial taxa such as Sphingomonas increased, whereas the proportion of pathogenic fungi such as Fusarium declined. Overall, this study provides a theoretical basis and practical reference for pest biological control and the sustainable development of grassland agriculture in the Ningxia region.
Key words
Alfalfa pests
/
Biological translation
/
Soil physical and chemical properties
/
Rhizosphere microecology
WANG Yu-zhe, CUI Jun-ling, WANG Jia-rui, LI Zhi-qiu, WANG Ying, SUN Wei, ZHANG Rong, BAN Li-ping, WEI Shu-hua.
Effects of Two Biocontrol Fungi on Major Alfalfa Pests and Rhizosphere Microecology[J].
Acta Agrestia Sinica,
2026
, 34
(7)
: 2429
-2439
.
DOI: 10.11733/j.issn.1007-0435.2026.07.008
ZHANG D, LONG H Y. Evaluation of production performance and nutritional value of eight alfalfa varieties in the hot-arid zone[J]. Chinese Journal of Grassland, 2024, 46(1):70-77 张德, 龙会英. 8个紫花苜蓿品种在干热区生产性能和营养价值评价[J]. 中国草地学报, 2024, 46(1):70-77 YUE Y H, QI X, WANG Y R, et al. Persistence of 35 Medicago sativa varieties at the 10th year after establishment[J]. Acta Prataculturae Sinica, 2014, 23(1):58-64 岳彦红, 齐晓, 王彦荣, 等. 35个10龄紫花苜蓿品种的持久性比较[J]. 草业学报, 2014, 23(1):58-64 CHU J X, ZHANG L Z. The quality characteristics and application of alfalfa in animal production[J]. China Feed, 2022(11):91-95 初建香, 张立中. 紫花苜蓿的品质特性及其在动物生产中的应用[J]. 中国饲料, 2022(11):91-95 WANG Q B, ZOU Y. China’s alfalfa market and imports: development, trends, and potential impacts of the U.S.-China trade dispute and retaliations[J]. Journal of Integrative Agriculture, 2020, 19(4):1149-1158 SUN Q Z, YU Z, XU C C. Urgency of further developing alfalfa industry in China[J]. Pratacultural Science, 2012(2):314-319 孙启忠, 玉柱, 徐春城. 我国苜蓿产业亟待振兴[J]. 草业科学, 2012(2):314-319 RODRÍGUEZ D, COY-BARRERA E. Overview of updated control tactics for western flower Thrips[J]. Insects, 2023, 14(7):649 LI N, MA X X, WANG X P. Research progress on a major insect pest of Alfalfa Odontothrips loti[J]. China Plant Protection, 2019, 39(11):31-35 李楠, 马晓霞, 王新谱. 苜蓿重要害虫牛角花齿蓟马研究进展[J]. 中国植保导刊, 2019, 39(11):31-35 QIAO L, ZHANG Z Q, BAN L P, et al. Optimization trapping technology for controlling Odontothrips loti(Haliday)(Thysanoptera, Thripidae) in alfalfa field[J]. Chinese Journal of Biological Control, 2023, 39(6):1351-1360 乔浪, 张志强, 班丽萍, 等. 紫花苜蓿田牛角花齿蓟马诱集技术优化研究[J]. 中国生物防治学报, 2023, 39(6):1351-1360 TANG L D, YAN K L, FU B L, et al. The life table parameters of Megalurothrips usitatus (Thysanoptera: Thripidae) on four leguminous crops[J]. The Florida Entomologist, 2015, 98(2):620-625 SRINIVASAN R, PAOLA S, LIN M Y, et al. Development and validation of an integrated pest management strategy for the control of major insect pests on yard-long bean in Cambodia[J]. Crop Protection, 2019, 116:82-91 FU Z Y, LUO Y N, MIAO M, et al. Predation of Orius similis on different instars of the spotted alfalfa aphid in alfalfa[J]. Chinese Journal of Biological Control:1-11. 傅祖英, 罗撄宁, 苗淼, 等. 南方小花蝽对苜蓿斑蚜不同龄期的捕食作用 [J]. 中国生物防治学报: 1-11. CUI X N, XI B X, PENG B, et al. Predatory functional response of Orius minutus to Acyrthosiphon pisum and Odontothrips loti at different temperatures[J]. Chinese Journal of Biological Control, 2025, 41(2):276-283 崔晓宁, 席驳鑫, 彭斌, 等. 不同温度下微小花蝽对豌豆蚜和牛角花齿蓟马的捕食作用[J]. 中国生物防治学报, 2025, 41(2):276-283 WANG P, LIU X G, HUANG Z Y. Laboratory bioassay of five insecticides on against field dominant populations of Thrips[J]. Agrochemicals, 2016, 55(7):527-529 王彭, 刘叙杆, 黄正谊. 5种药剂对不同地区蓟马田间优势种群的敏感度测定[J]. 农药, 2016, 55(7):527-529 YE H C, WANG Q, YAN C, et al. The field efficacy and joint toxicity of emamectin benzoate and acetamiprid against cowpea Thrips[J]. Chinese Journal of Tropical Agriculture, 2020, 40(2):76-81 叶火春, 王琴, 闫超, 等. 甲维盐与啶虫脒对豇豆蓟马的联合毒力及田间防效[J]. 热带农业科学, 2020, 40(2):76-81 LIU K, TANG L D, LI P, et al. Toxocity and synergistic effect of the complex formulation of several insecticides to Megalurothrips usitatus (bagnall)[J]. Chinese Journal of Tropical Crops, 2014, 35(8):1615-1618 刘奎, 唐良德, 李鹏, 等. 几种杀虫剂对豆大蓟马的毒力测定及复配增效作用[J]. 热带作物学报, 2014, 35(8):1615-1618 LUO Y N, HAN C, TU X B, et al. Evaluation of trap systems for monitoring of Odontothrips loti and Frankliniella occidentalis: a pilot field trial[J]. Insects, 2026, 17(1):84 YAN S, REN X Y, WANG D J, et al. The research progress on the effects of entomopathogenic fungi on natural enemies[J]. Chinese Journal of Biological Control, 2023, 39(1):221-230 严森, 任小云, 王登杰, 等. 昆虫病原真菌在害虫防治中对天敌生物的影响研究进展[J]. 中国生物防治学报, 2023, 39(1):221-230 LI J, XIA Y X. Host-pathogen interactions between Metarhizium spp. and locusts[J]. Journal of Fungi, 2022, 8(6):602 PENG G X, ZHANG S L, XIA Y X. Metarhizium anisopliae CQMa421 and its application status[J]. Chinese Journal of Biological Control, 2020, 36(6):850-857 彭国雄, 张淑玲, 夏玉先. 金龟子绿僵菌CQMa421农药及应用情况[J]. 中国生物防治学报, 2020, 36(6):850-857 WU S Y, HE Z, WANG E D, et al. Application of Beauveria bassiana and Neoseiulus barkeri for improved control of Frankliniella occidentalis in greenhouse cucumber[J]. Crop Protection, 2017, 96:83-87 TADESSE MAWCHA K, MALINGA L, MUIR D, et al. Recent advances in biopesticide research and development with a focus on microbials[J]. F1000Research, 2025, 13:1071 WANG X W, JIANG X D, DING X H, et al. Biological characteristics and pathogenicity of four dominant pathogenic fungi of overwintering larvae of Ostrinia furnacalis in Xinjiang Desert oasis region[J]. Chinese Journal of Biological Control, 2023, 39(2):305-312 王小武, 蒋旭东, 丁新华, 等. 新疆荒漠绿洲区亚洲玉米螟越冬幼虫4种主要病原真菌生物学特性及致病力测定[J]. 中国生物防治学报, 2023, 39(2):305-312 CHI X X, CAO W, WANG C, et al. Effects of combined restoration measures on soil enzyme activities and microbial communities in degraded meadow steppe[J]. Acta Agrestia Sinica, 2026, 34(4): 1403-1412 迟晓雪, 曹薇, 王畅, 等. 复合改良措施对退化草甸草原土壤酶活性与微生物群落的影响[J]. 草地学报, 2026, 34(4): 1403-1412 TAN H Y, CHEN B R, YAN R R, et al. Research progress on grassland soil microbial characteristics and their responses to anthropogenic disturbance[J]. Acta Agrestia Sinica, 2014, 22(6): 1163-1170 谭红妍, 陈宝瑞, 闫瑞瑞, 等. 草地土壤微生物特性及其对人为干扰响应的研究进展[J]. 草地学报, 2014, 22(6): 1163-1170 JABER L R, OWNLEY B H. Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens?[J]. Biological Control, 2018, 116:36-45 BEHIE S W, MOREIRA C C, SEMENTCHOUKOVA I, et al. Carbon translocation from a plant to an insect-pathogenic endophytic fungus[J]. Nature Communications, 2017, 8:14245 WEI Q Y, LI Y Y, XU C, et al. Endophytic colonization by Beauveria bassiana increases the resistance of tomatoes against Bemisia tabaci[J]. Arthropod-Plant Interactions, 2020, 14(3):289-300 ZHANG Q K, WANG D J, LIU S, et al. Reproductive disruption induced by Beauveria bassiana in Frankliniellaoccidentalis as a potential long-term biocontrol strategy[J]. Pesticide Biochemistry and Physiology, 2025, 212:106464 MIR Y H, AHMAD GANIE M, SHAH T I, et al. Soil microbial and enzyme activities in different land use systems of the Northwestern Himalayas[J]. PeerJ, 2023, 11:e15993 BARELLI L, WALLER A S, BEHIE S W, et al. Plant microbiome analysis after Metarhizium amendment reveals increases in abundance of plant growth-promoting organisms and maintenance of disease-suppressive soil[J]. Plos One, 2020, 15(4):e0231150 PAVA-RIPOLL M, ANGELINI C, FANG W G, et al. The rhizosphere-competent entomopathogen Metarhizium anisopliae expresses a specific subset of genes in plant root exudate[J]. Microbiology, 2011, 157(1):47-55 DU W, ZHOU J, JIANG P, et al. Effects of Beauveria bassiana and acephate on enzyme activities and microbial diversity in paddy soil[J]. Plant, Soil and Environment, 2013, 59(12):562-567 ZAHN D K, HAVILAND D R, STANGHELLINI M E, et al. Evaluation of Beauveria bassiana for management of Citrus thrips (Thysanoptera: Thripidae) in California blueberries[J]. Journal of Economic Entomology, 2013, 106(5):1986-1995 BRUCK D J. Ecology of Metarhizium anisopliae in soilless potting media and the rhizosphere: implications for pest management[J]. Biological Control, 2005, 32(1):155-163