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To investigate the response mechanisms of heteromorphic seeds of Cenchrus spinifex, we examined germination characteristics of its two seed types (M-type: larger; P-type: smaller) under varying light (complete darkness vs. 12 h light/12 h dark) and moisture conditions (simulated by PEG gradients). Subsequent seedling growth, biomass allocation, and phenotypic plasticity were analyzed in response to the interaction between water availability (normal, mild, and severe drought) and light intensity (high and low intensity). The results showed that germination was unaffected by light but strongly inhibited by water stress; under severe drought, M-type seeds outperformed P-types in root length, bud length, and biomass. As seedlings, M-type plants showed higher phenotypic plasticity in morphological traits (plant height, tiller number, leaf area), favoring increased aboveground resource investment for rapid life cycle completion, whereas P-type plants exhibited greater plasticity in biomass allocation, enhancing stress tolerance through increased root biomass proportion. In summary, the complementary germination and growth strategies of heteromorphic seeds in C. spinifex represent an effective “bet-hedging” mechanism, which is crucial for its successful invasion and spread across environmentally stressful and variable habitats., authors=CAO Jing1,2, WANG Wen-hui1, LI Zhen-song3, RONG Yu-ping2, authorsList=CAO Jing, WANG Wen-hui, LI Zhen-song, RONG Yu-ping, authorCompany=1 College of Grassland, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China; 2 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China; 3 Qingdao Hengxing University, Qingdao, Shandong Province 266100, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574773340988364, articleId=1309574771986228171, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=少花蒺藜草异型种子对光照和水分条件的差异化适应策略研究, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=种子异型性是入侵植物适应环境异质性的关键策略。为探究少花蒺藜草(Cenchrus spinifex Cav.)异型种子对环境因子的响应机制,本研究通过控制光照(全黑暗与12 h光照/12 h黑暗)和水分胁迫[聚乙二醇(Polyethylene glycol,PEG)模拟]观察异型种子(M型:大种子;P型:小种子)的萌发特性,并通过水分(正常、轻度干旱、重度干旱)与光照(高光、低光)交互作用分析幼苗生长、生物量分配及表型可塑性差异。结果表明:异型种子萌发率不受光照影响但受水分胁迫显著抑制;重度干旱下M型种子萌发能力更强且胚根、胚芽及生物量表现优于P型。M型植株在株高、分蘖、叶面积等形态性状上表型可塑性更高,倾向于增加地上资源投入以快速完成生活史;P型植株则在生物量分配格局上更具可塑性,通过提升根系占比增强胁迫耐受能力。研究表明,少花蒺藜草异型种子通过萌发与生长策略的“两头下注”形成适应机制,为其在逆境中成功入侵和扩展提供了生态基础。, authors=曹婧1,2, 王文慧1, 李振松3, 戎郁萍2, authorsList=曹婧, 王文慧, 李振松, 戎郁萍, authorCompany=1 青岛农业大学草业学院, 山东 青岛 266109; 2 中国农业大学草业科学与技术学院, 北京 100193; 3 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Bet-hedging strategies of native and exotic annuals promote coexistence in semiarid Chile[J]. Journal of Arid Environments, 2016, 126:62-67 WANG L, DONG M, HUANG Z Y. Review of research on seed heteromorphism and its ecological significance[J]. Chinese Journal of Plant Ecology, 2010, 34(5):578-590 王雷, 董鸣, 黄振英. 种子异型性及其生态意义的研究进展[J]. 植物生态学报, 2010, 34(5):578-590 WEI Y, DONG M, HUANG Z Y. Seed polymorphism, dormancy and germination of Salsola affinis (Chenopodiaceae), a dominant desert annual inhabiting the Junggar Basin of Xinjiang, China[J]. Australian Journal of Botany, 2007, 55(4):464-470 FUNK J L. Differences in plasticity between invasive and native plants from a low resource environment[J]. Journal of Ecology, 2008, 96(6):1162-1173 CAO D C, BASKIN C C, BASKIN J M, et al. Comparison of germination and seed bank dynamics of dimorphic seeds of the cold desert halophyte Suaeda corniculata subsp. mongolica[J]. Annals of Botany, 2012, 110(8):1545-1558 YAO S X, LAN H Y, ZHANG F C. Variation of seed heteromorphism in Chenopodium album and the effect of salinity stress on the descendants[J]. Annals of Botany, 2010, 105(6):1015-1025 VALLADARES F, GIANOLI E, GÓMEZ J M. Ecological limits to plant phenotypic plasticity[J]. The New Phytologist, 2007, 176(4):749-763 HIATT D, FLORY S L. Populations of a widespread invader and co-occurring native species vary in phenotypic plasticity[J]. New Phytologist, 2020, 225(1):584-594 BOSSDORF O, AUGE H, LAFUMA L, et al. Phenotypic and genetic differentiation between native and introduced plant populations[J]. Oecologia, 2005, 144(1):1-11 MATESANZ S, HORGAN-KOBELSKI T, SULTAN S E. Phenotypic plasticity and population differentiation in an ongoing species invasion[J]. PLoS One, 2012, 7(9):e44955 ZHANG Y L, ZHANG R H, FU W D, et al. Effects of different cultivation practices on the amount of seeds in the soils and seed production of Cenchrus pauciflorus Benth[J]. Journal of Agricultural Resources and Environment, 2015, 32(3):312-320 张衍雷, 张瑞海, 付卫东, 等. 不同农作措施对少花蒺藜草(Cenchrus pauciflorus Benth)种子库及其繁殖能力的影响[J]. 农业资源与环境学报, 2015, 32(3):312-320 AN R J, WANG Y Z, TIAN X. Research progress of the invasive plant—Cenchrus pauciflorus[J]. Weed Science, 2015, 33(1):27-31 安瑞军, 王永忠, 田迅. 外来入侵植物——少花蒺藜草研究进展[J]. 杂草科学, 2015, 33(1):27-31 SUN Z L, SHU Q, GAO K, et al. Invasion status, adaptive mechanism and control strategy of field sandbur: a review[J]. Acta Agrestia Sinica, 2020, 28(5):1196-1202 孙忠林, 淑琴, 高凯, 等. 少花蒺藜草入侵现状、适应机制和防控策略[J]. 草地学报, 2020, 28(5):1196-1202 QU T, TIAN X, ZHOU L Y. Seed heteromorphism and population maintenance strategy of invasive plant Cenchrus pauciflorus[J]. Chinese Journal of Ecology, 2020, 39(8):2622-2628 曲婷, 田迅, 周立业. 入侵植物少花蒺藜草种子异型性及种群维持策略[J]. 生态学杂志, 2020, 39(8):2622-2628 PENNACCHI J P, LIRA J M S, RODRIGUES M, et al. A systemic approach to the quantification of the phenotypic plasticity of plant physiological traits: the multivariate plasticity index[J]. Journal of Experimental Botany, 2021, 72(5):1864-1878 LIU R R, WANG L, TANVEER M, et al. Seed heteromorphism: an important adaptation of halophytes for habitat heterogeneity[J]. Frontiers in Plant Science, 2018, 9:1515 MILBERG P, ANDERSSON L, THOMPSON K. Large-seeded spices are less dependent on light for germination than small-seeded ones[J]. Seed Science Research, 2000, 10(1):99-104 ZHANG M, ZHU J J, YAN Q L. Review on influence mechanisms of light in seed germination: review on influence mechanisms of light in seed germination[J]. Chinese Journal of Plant Ecology, 2012, 36(8):899-908 SONG K, CHOI G. Phytochrome regulation of seed germination[M]//Phytochromes. New York, NY:Springer New York, 2019:149-156 QU T, ZHOU L Y. Seed germination strategy and seedling growth characteristics of heteromorphic seeds of the invasive weed Cenchrus incertus[J]. Acta Prataculturae Sinica, 2022, 31(6):91-100 曲婷, 周立业. 入侵植物少花蒺藜草异型种子萌发策略及其幼苗生长特性[J]. 草业学报, 2022, 31(6):91-100 ACOSTA Y, PÉREZ L, ESCALANTE D, et al. Heteromorphic seed germination and seedling emergence in the legume Teramnus labialis (L.f.) Spreng (Fabacaeae)[J]. Botany, 2020, 98(7):371-379 CARTER C T, UNGAR I A. Germination response of dimorphic seeds of two halophyte species to environmentally controlled and natural conditions[J]. Canadian Journal of Botany, 2003, 81(9):918-926 ABLEY K, GOSWAMI R, LOCKE J C W. Bet-hedging and variability in plant development: seed germination and beyond[J]. Philosophical Transactions of the Royal Society B:Biological Sciences, 2024, 379(1900):20230048 MATSUO M, SAKAI T, KAWANO Y. Seed heteromorphism in Carolina dayflower (Commelina caroliniana Walter)[J]. Weed Biology and Management, 2016, 16(4):169-176 SIMONS A M, JOHNSTON M O. Environmental and genetic sources of diversification in the timing of seed germination: implications for the evolution of bet hedging[J]. Evolution, 2006, 60(11):2280-2292 LEVERETT L D, JOLLS C L. Cryptic seed heteromorphism in Packera tomentosa (Asteraceae): differences in mass and germination[J]. Plant Species Biology, 2014, 29(2):169-180 SIMONS A M. Modes of response to environmental change and the elusive empirical evidence for bet hedging[J]. Proceedings:Biological Sciences, 278(1712):1601-1609 VELIJEVIC N, STRBANOVIC R, POSTIC D, et al. Effects of seed coat colour on the seed quality and initial seedling growth of red clover cultivars (Trifolium pratense)[J]. Journal on Processing and Energy in Agriculture, 2017, 21(3):174-177 KADEREIT G, NEWTON R J, VANDELOOK F. Evolutionary ecology of fast seed germination—a case study in Amaranthaceae/Chenopodiaceae[J]. Perspectives in Plant Ecology, Evolution and Systematics, 2017, 29:1-11 COMAS L H, BECKER S R, CRUZ V M V, et al. Root traits contributing to plant productivity under drought[J]. Frontiers in Plant Science, 2013, 4:442 SCHOLL J P, CALLE L, MILLER N, et al. Offspring polymorphism and bet hedging: a large-scale, phylogenetic analysis[J]. Ecology Letters, 2020, 23(8):1223-1231 STARRFELT J, KOKKO H. Bet-hedging—a triple trade-off between means, variances and correlations[J]. Biological Reviews, 2012, 87(3):742-755 SIEBENKÄS A, SCHUMACHER J, ROSCHER C. Phenotypic plasticity to light and nutrient availability alters functional trait ranking across eight perennial grassland species[J]. AoB Plants, 2015, 7:plv029 ZENG Y O. Research progress on the effects of light on the growth and biomass of plant branches and leaves[J]. Agricultural Technology & Equipment, 2022(12):57-59 曾译欧. 光照对植物枝叶生长和生物量的影响研究进展[J]. 农业技术与装备, 2022(12):57-59 XU D Q, ZHANG Y Z, ZHANG R X. Photoinhibition of photosynthesis in plants[J]. Plant Physiology Communications, 1992, 28(4):237-243 许大全, 张玉忠, 张荣铣. 植物光合作用的光抑制[J]. 植物生理学通讯, 1992, 28(4):237-243 WANG D D, GAO J H, WANG X Y. Research on the stress adaptation of plant physiological ecology of photosynthesis[J]. China Rural Water and Hydropower, 2010(8):43-45, 47 王多栋, 高金晖, 王晓英. 植物对逆境的光合生理生态适应性研究[J]. 中国农村水利水电, 2010(8):43-45, 47 ZHANG Z X, TIAN X. Characteristics of biomass allocation of Cenchrus pauciflorus under arid and irrigated habitats[J]. Pratacultural Science, 2011, 28(2):185-188 张志新, 田迅. 干旱和灌溉条件下少花蒺藜草分株生物量分配特征[J]. 草业科学, 2011, 28(2):185-188 VÁZQUEZ D P, GIANOLI E, MORRIS W F, et al. Ecological and evolutionary impacts of changing climatic variability[J]. Biological Reviews, 2017, 92(1):22-42 VALLADARES F, MATESANZ S, GUILHAUMON F, et al. The effects of phenotypic plasticity and local adaptation on forecasts of species range shifts under climate change[J]. Ecology Letters, 2014, 17(11):1351-1364 CAO J, XU J, PAN X B, et al. Potential impact of climate change on the global geographical distribution of the invasive species, Cenchrus spinifex (Field sandbur, Gramineae)[J]. Ecological Indicators, 2021, 131:108204 YANNELLI F A, HUGHES P, KOLLMANN J.Preventing plant invasions at early stages of revegetation: the role of limiting similarity in seed size and seed density[J]. Ecological Engineering, 2017, 100:286-290 MORAIS M C, FREITAS H. The acclimation potential of Acacia longifolia to water stress: implications for invasiveness[J]. Plant Science, 2012, 196:77-84)
Divergent Adaptation of Cenchrus spinifex Heteromorphic Seeds to Light and Water Conditions
CAO Jing1,2, WANG Wen-hui1, LI Zhen-song3, RONG Yu-ping2
Affiliations
1 College of Grassland, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China; 2 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China; 3 Qingdao Hengxing University, Qingdao, Shandong Province 266100, China
Seed heteromorphism is a critical adaptive strategy for invasive plants to cope with environmental heterogeneity. To investigate the response mechanisms of heteromorphic seeds of Cenchrus spinifex, we examined germination characteristics of its two seed types (M-type: larger; P-type: smaller) under varying light (complete darkness vs. 12 h light/12 h dark) and moisture conditions (simulated by PEG gradients). Subsequent seedling growth, biomass allocation, and phenotypic plasticity were analyzed in response to the interaction between water availability (normal, mild, and severe drought) and light intensity (high and low intensity). The results showed that germination was unaffected by light but strongly inhibited by water stress; under severe drought, M-type seeds outperformed P-types in root length, bud length, and biomass. As seedlings, M-type plants showed higher phenotypic plasticity in morphological traits (plant height, tiller number, leaf area), favoring increased aboveground resource investment for rapid life cycle completion, whereas P-type plants exhibited greater plasticity in biomass allocation, enhancing stress tolerance through increased root biomass proportion. In summary, the complementary germination and growth strategies of heteromorphic seeds in C. spinifex represent an effective “bet-hedging” mechanism, which is crucial for its successful invasion and spread across environmentally stressful and variable habitats.
CAO Jing, WANG Wen-hui, LI Zhen-song, RONG Yu-ping.
Divergent Adaptation of Cenchrus spinifex Heteromorphic Seeds to Light and Water Conditions[J].
Acta Agrestia Sinica,
2026
, 34
(7)
: 2393
-2402
.
DOI: 10.11733/j.issn.1007-0435.2026.07.005
JIMÉNEZ M A, GAXIOLA A, ARMESTO J J, et al. Bet-hedging strategies of native and exotic annuals promote coexistence in semiarid Chile[J]. Journal of Arid Environments, 2016, 126:62-67 WANG L, DONG M, HUANG Z Y. Review of research on seed heteromorphism and its ecological significance[J]. Chinese Journal of Plant Ecology, 2010, 34(5):578-590 王雷, 董鸣, 黄振英. 种子异型性及其生态意义的研究进展[J]. 植物生态学报, 2010, 34(5):578-590 WEI Y, DONG M, HUANG Z Y. Seed polymorphism, dormancy and germination of Salsola affinis (Chenopodiaceae), a dominant desert annual inhabiting the Junggar Basin of Xinjiang, China[J]. Australian Journal of Botany, 2007, 55(4):464-470 FUNK J L. Differences in plasticity between invasive and native plants from a low resource environment[J]. Journal of Ecology, 2008, 96(6):1162-1173 CAO D C, BASKIN C C, BASKIN J M, et al. Comparison of germination and seed bank dynamics of dimorphic seeds of the cold desert halophyte Suaeda corniculata subsp. mongolica[J]. Annals of Botany, 2012, 110(8):1545-1558 YAO S X, LAN H Y, ZHANG F C. Variation of seed heteromorphism in Chenopodium album and the effect of salinity stress on the descendants[J]. Annals of Botany, 2010, 105(6):1015-1025 VALLADARES F, GIANOLI E, GÓMEZ J M. Ecological limits to plant phenotypic plasticity[J]. The New Phytologist, 2007, 176(4):749-763 HIATT D, FLORY S L. Populations of a widespread invader and co-occurring native species vary in phenotypic plasticity[J]. New Phytologist, 2020, 225(1):584-594 BOSSDORF O, AUGE H, LAFUMA L, et al. Phenotypic and genetic differentiation between native and introduced plant populations[J]. Oecologia, 2005, 144(1):1-11 MATESANZ S, HORGAN-KOBELSKI T, SULTAN S E. Phenotypic plasticity and population differentiation in an ongoing species invasion[J]. PLoS One, 2012, 7(9):e44955 ZHANG Y L, ZHANG R H, FU W D, et al. Effects of different cultivation practices on the amount of seeds in the soils and seed production of Cenchrus pauciflorus Benth[J]. Journal of Agricultural Resources and Environment, 2015, 32(3):312-320 张衍雷, 张瑞海, 付卫东, 等. 不同农作措施对少花蒺藜草(Cenchrus pauciflorus Benth)种子库及其繁殖能力的影响[J]. 农业资源与环境学报, 2015, 32(3):312-320 AN R J, WANG Y Z, TIAN X. Research progress of the invasive plant—Cenchrus pauciflorus[J]. Weed Science, 2015, 33(1):27-31 安瑞军, 王永忠, 田迅. 外来入侵植物——少花蒺藜草研究进展[J]. 杂草科学, 2015, 33(1):27-31 SUN Z L, SHU Q, GAO K, et al. Invasion status, adaptive mechanism and control strategy of field sandbur: a review[J]. Acta Agrestia Sinica, 2020, 28(5):1196-1202 孙忠林, 淑琴, 高凯, 等. 少花蒺藜草入侵现状、适应机制和防控策略[J]. 草地学报, 2020, 28(5):1196-1202 QU T, TIAN X, ZHOU L Y. Seed heteromorphism and population maintenance strategy of invasive plant Cenchrus pauciflorus[J]. Chinese Journal of Ecology, 2020, 39(8):2622-2628 曲婷, 田迅, 周立业. 入侵植物少花蒺藜草种子异型性及种群维持策略[J]. 生态学杂志, 2020, 39(8):2622-2628 PENNACCHI J P, LIRA J M S, RODRIGUES M, et al. A systemic approach to the quantification of the phenotypic plasticity of plant physiological traits: the multivariate plasticity index[J]. Journal of Experimental Botany, 2021, 72(5):1864-1878 LIU R R, WANG L, TANVEER M, et al. Seed heteromorphism: an important adaptation of halophytes for habitat heterogeneity[J]. Frontiers in Plant Science, 2018, 9:1515 MILBERG P, ANDERSSON L, THOMPSON K. Large-seeded spices are less dependent on light for germination than small-seeded ones[J]. Seed Science Research, 2000, 10(1):99-104 ZHANG M, ZHU J J, YAN Q L. Review on influence mechanisms of light in seed germination: review on influence mechanisms of light in seed germination[J]. Chinese Journal of Plant Ecology, 2012, 36(8):899-908 SONG K, CHOI G. Phytochrome regulation of seed germination[M]//Phytochromes. New York, NY:Springer New York, 2019:149-156 QU T, ZHOU L Y. Seed germination strategy and seedling growth characteristics of heteromorphic seeds of the invasive weed Cenchrus incertus[J]. Acta Prataculturae Sinica, 2022, 31(6):91-100 曲婷, 周立业. 入侵植物少花蒺藜草异型种子萌发策略及其幼苗生长特性[J]. 草业学报, 2022, 31(6):91-100 ACOSTA Y, PÉREZ L, ESCALANTE D, et al. Heteromorphic seed germination and seedling emergence in the legume Teramnus labialis (L.f.) Spreng (Fabacaeae)[J]. Botany, 2020, 98(7):371-379 CARTER C T, UNGAR I A. Germination response of dimorphic seeds of two halophyte species to environmentally controlled and natural conditions[J]. Canadian Journal of Botany, 2003, 81(9):918-926 ABLEY K, GOSWAMI R, LOCKE J C W. Bet-hedging and variability in plant development: seed germination and beyond[J]. Philosophical Transactions of the Royal Society B:Biological Sciences, 2024, 379(1900):20230048 MATSUO M, SAKAI T, KAWANO Y. Seed heteromorphism in Carolina dayflower (Commelina caroliniana Walter)[J]. Weed Biology and Management, 2016, 16(4):169-176 SIMONS A M, JOHNSTON M O. Environmental and genetic sources of diversification in the timing of seed germination: implications for the evolution of bet hedging[J]. Evolution, 2006, 60(11):2280-2292 LEVERETT L D, JOLLS C L. Cryptic seed heteromorphism in Packera tomentosa (Asteraceae): differences in mass and germination[J]. Plant Species Biology, 2014, 29(2):169-180 SIMONS A M. Modes of response to environmental change and the elusive empirical evidence for bet hedging[J]. Proceedings:Biological Sciences, 278(1712):1601-1609 VELIJEVIC N, STRBANOVIC R, POSTIC D, et al. Effects of seed coat colour on the seed quality and initial seedling growth of red clover cultivars (Trifolium pratense)[J]. Journal on Processing and Energy in Agriculture, 2017, 21(3):174-177 KADEREIT G, NEWTON R J, VANDELOOK F. Evolutionary ecology of fast seed germination—a case study in Amaranthaceae/Chenopodiaceae[J]. Perspectives in Plant Ecology, Evolution and Systematics, 2017, 29:1-11 COMAS L H, BECKER S R, CRUZ V M V, et al. Root traits contributing to plant productivity under drought[J]. Frontiers in Plant Science, 2013, 4:442 SCHOLL J P, CALLE L, MILLER N, et al. Offspring polymorphism and bet hedging: a large-scale, phylogenetic analysis[J]. Ecology Letters, 2020, 23(8):1223-1231 STARRFELT J, KOKKO H. Bet-hedging—a triple trade-off between means, variances and correlations[J]. Biological Reviews, 2012, 87(3):742-755 SIEBENKÄS A, SCHUMACHER J, ROSCHER C. Phenotypic plasticity to light and nutrient availability alters functional trait ranking across eight perennial grassland species[J]. AoB Plants, 2015, 7:plv029 ZENG Y O. Research progress on the effects of light on the growth and biomass of plant branches and leaves[J]. Agricultural Technology & Equipment, 2022(12):57-59 曾译欧. 光照对植物枝叶生长和生物量的影响研究进展[J]. 农业技术与装备, 2022(12):57-59 XU D Q, ZHANG Y Z, ZHANG R X. Photoinhibition of photosynthesis in plants[J]. Plant Physiology Communications, 1992, 28(4):237-243 许大全, 张玉忠, 张荣铣. 植物光合作用的光抑制[J]. 植物生理学通讯, 1992, 28(4):237-243 WANG D D, GAO J H, WANG X Y. Research on the stress adaptation of plant physiological ecology of photosynthesis[J]. China Rural Water and Hydropower, 2010(8):43-45, 47 王多栋, 高金晖, 王晓英. 植物对逆境的光合生理生态适应性研究[J]. 中国农村水利水电, 2010(8):43-45, 47 ZHANG Z X, TIAN X. Characteristics of biomass allocation of Cenchrus pauciflorus under arid and irrigated habitats[J]. Pratacultural Science, 2011, 28(2):185-188 张志新, 田迅. 干旱和灌溉条件下少花蒺藜草分株生物量分配特征[J]. 草业科学, 2011, 28(2):185-188 VÁZQUEZ D P, GIANOLI E, MORRIS W F, et al. Ecological and evolutionary impacts of changing climatic variability[J]. Biological Reviews, 2017, 92(1):22-42 VALLADARES F, MATESANZ S, GUILHAUMON F, et al. The effects of phenotypic plasticity and local adaptation on forecasts of species range shifts under climate change[J]. Ecology Letters, 2014, 17(11):1351-1364 CAO J, XU J, PAN X B, et al. Potential impact of climate change on the global geographical distribution of the invasive species, Cenchrus spinifex (Field sandbur, Gramineae)[J]. Ecological Indicators, 2021, 131:108204 YANNELLI F A, HUGHES P, KOLLMANN J.Preventing plant invasions at early stages of revegetation: the role of limiting similarity in seed size and seed density[J]. Ecological Engineering, 2017, 100:286-290 MORAIS M C, FREITAS H. The acclimation potential of Acacia longifolia to water stress: implications for invasiveness[J]. Plant Science, 2012, 196:77-84