草地学报

星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析

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草地学报 | 研究论文 2026,34(7): 2366-2376

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草地学报 |研究论文 2026 , 34 (7) : 2366 -2376

星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析

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马蓉¹, 杨攀¹, 张蕴文², 胡晓炜¹, 王会², 王沛¹

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作者简介:

马蓉（2000-），女，回族，宁夏中宁人，硕士研究生，主要从事牧草逆境生理与分子生物学研究，E-mail:18328569843@163.com

Cloning of PtCASP1 Gene in Puccinellia tenuiflora and Analysis of its Expression Patterns under Saline and Stagnant Stress

MA Rong¹, YANG Pan¹, ZHANG Yun-wen², HU Xiao-wei¹, WANG Hui², WANG Pei¹

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doi: 10.11733/j.issn.1007-0435.2026.07.003

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摘要

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星星草（Puccinellia tenuiflora）是一种对盐涝交互逆境有独特适应性的盐生植物，内皮层加厚形成的质外体屏障可能是其适应这一环境的关键。凯氏带和木栓层是根内皮层水和溶质径向运输的质外体屏障。本研究以星星草为材料，通过基因克隆获得凯氏带形成关键基因 PtCASP1，并对该基因进行生物信息学分析、亚细胞定位及盐、涝和盐涝复合胁迫下的表达模式分析。结果表明，PtCASP1基因全长 552 bp，编码184个氨基酸，为疏水性稳定蛋白，具有响应赤霉素、干旱、低温及光反应等顺式作用元件。系统进化分析表明PtCASP1与多花黑麦草（Lolium multiflorum）、看麦娘（Alopecurus aequalis）的亲缘关系较近。免疫荧光分析显示，PtCASP1 蛋白分布于细胞核与细胞膜。PtCASP1 基因在根中表达量显著高于叶，且受盐、涝及盐涝复合胁迫诱导，均在各处理 72 h 时表达量最高。本研究可为今后深入研究凯氏带在星星草适应盐涝交互逆境中的功能提供参考。

关键词

星星草 / PtCASP1 / 基因克隆 / 亚细胞定位 / 表达分析 / 盐涝复合胁迫

Abstract

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Puccinellia tenuiflora is a halophyte with remarkable adaptability to the combined stress of saline and stagnant. The apoplastic barrier formed by endodermal thickening is likely crucial for this adaptation， in which the Casparian strip and suberin lamellae serve as key apoplastic barriers in endodermis for the radial transport of water and solutes. In this study， using Puccinellia tenuiflora as material， we cloned PtCASP1， a key gene involved in Casparian strip formation. Bioinformatic analysis， subcellular localization， and expression pattern analysis under saline， stagnant and combined saline-stagnant stresses were conducted. The results showed that the PtCASP1 gene was 552 bp in length， encoding 184 amino acids. It was a hydrophobic， stable protein and contained cis-acting elements responsive to gibberellin， drought stress， low temperature stress， and light response. Phylogenetic analysis indicated that PtCASP1 was closely related to those in Lolium multiflorum and Alopecurus aequalis. Immunofluorescence analysis revealed that the PtCASP1 protein exhibited dual localization in the nucleus and cell membrane. The expression level of the PtCASP1 gene in the roots of Puccinellia tenuiflora was significantly higher than that in the leaves， and it responded to saline， stagnant， and combined saline-stagnant stresses， with the highest relative expression levels at 72 hours post-treatment for all stress conditions. This study provides a reference for further investigation into the function of the Casparian strip in helping Puccinellia tenuiflora adapt to saline-stagnant combined stress.

Key words

Puccinellia tenuiflora / PtCASP1 / Gene cloning / Subcellular localization / Expression analysis / Combined saline-stagnant stress

引用本文

马蓉, 杨攀, 张蕴文, 胡晓炜, 王会, 王沛. 星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析. 草地学报, 2026 , 34 (7) : 2366 -2376 . DOI: 10.11733/j.issn.1007-0435.2026.07.003

MA Rong, YANG Pan, ZHANG Yun-wen, HU Xiao-wei, WANG Hui, WANG Pei. Cloning of PtCASP1 Gene in Puccinellia tenuiflora and Analysis of its Expression Patterns under Saline and Stagnant Stress[J]. Acta Agrestia Sinica, 2026 , 34 (7) : 2366 -2376 . DOI: 10.11733/j.issn.1007-0435.2026.07.003

基金

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四川省科技计划项目 (2024NSFSC0310); 西南民族大学“双一流”项目 (CX2023013); 西南民族大学青藏高原研究院创新团队项目 (2024CXTD11)

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2026年第34卷第7期

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doi: 10.11733/j.issn.1007-0435.2026.07.003

接收时间：2025-11-03
首发时间：2026-09-23

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收稿日期：2025-11-03
修回日期：2025-12-14

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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