草地学报

草地退化对土壤碳循环、微生物功能基因及代谢的影响

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草地学报 | 研究论文 2026,34(7): 2341-2354

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草地学报 |研究论文 2026 , 34 (7) : 2341 -2354

草地退化对土壤碳循环、微生物功能基因及代谢的影响

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丁成翔¹, 李兴福², 苏德荣³, 刘禹⁴

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丁成翔（1980-），男，回族，宁夏中卫人，博士，主要从事草地生态学研究，E-mail:2011990016@qhu.edu.cn

Effects of Grassland Degradation on Soil Carbon Cycle， Microbial Functional Genes and Metabolism

DING Cheng-xiang¹, LI Xing-fu², SU De-rong³, LIU Yu⁴

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doi: 10.11733/j.issn.1007-0435.2026.07.001

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在全球气候变化背景下，草地退化对土壤碳循环的影响机制仍缺乏系统认知。本研究以青藏高原15对未退化—退化配对样地为对象，结合微宇宙培养、宏基因组与宏代谢组分析，综合评估退化对土壤碳循环关键过程、有机碳组分、微生物功能基因及代谢产物的影响。结果表明：草地退化显著降低活性碳库（颗粒有机碳、溶解性有机碳和微生物生物量碳分别下降48.21%、30.87%和20.21%），同时增强有机碳矿化（提高56.71%），降低碳积累效率（下降32.51%）。退化还导致碳循环功能基因多样性下降（-2.95%，P<0.05），但淀粉分解基因及碳水化合物代谢产物显著增加（分别上升9.39%和49.6%，P<0.05），表明微生物碳限制加剧，代谢策略由合成向分解转变。本研究从多要素耦合视角阐明了草地退化调控土壤碳循环的关键机制，表明环境因子与微生物特征协同驱动碳循环变化，其中微生物功能基因与代谢产物发挥主导作用。

关键词

土壤碳循环 / 高寒草地 / 青藏高原 / 草地退化 / 功能基因 / 微生物代谢

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Under the context of global climate change， the influence mechanism of grassland degradation on soil carbon cycling remain poorly understood. In this study， we investigated 15 paired degraded and non-degraded grassland sites on the Qinghai-Tibet Plateau， integrated microcosm incubation， metagenomics and metabolomics to assess the impacts of degradation on key carbon cycling processes， organic carbon fractions， microbial functional genes， and metabolic intermediates. The results showed that grassland degradation significantly reduced labile carbon pools， with particulate organic carbon， dissolved organic carbon， and microbial biomass carbon decreasing by 48.21%， 30.87%， and 20.21%， respectively. Meanwhile， soil organic carbon mineralization increased by 56.71%， and carbon accumulation efficiency declined by 32.51%. Degradation also led to a decrease in the diversity of carbon cycling functional genes （-2.95%， P<0.05）， while the abundance of starch-degrading genes and carbohydrate-related metabolites increased significantly （by 9.39% and 49.6%， respectively）. These changes indicated that intensified microbial carbon limitation and a shift in metabolic strategy from synthesis to decoomposition. This study elucidates the key mechanisms underlying soil carbon cycling responses to grassland degradation from a multi-factor coupling perspective， revealing that environmental factors and microbial traits jointly regulated carbon cycling， with microbial functional genes and metabolic intermediates playing a dominat role.

Key words

Soil carbon cycling / Alpine grassland / Qinghai-Xizang Plateau / Grassland degradation / Functional genes / Microbial metabolism

引用本文

丁成翔, 李兴福, 苏德荣, 刘禹. 草地退化对土壤碳循环、微生物功能基因及代谢的影响. 草地学报, 2026 , 34 (7) : 2341 -2354 . DOI: 10.11733/j.issn.1007-0435.2026.07.001

DING Cheng-xiang, LI Xing-fu, SU De-rong, LIU Yu. Effects of Grassland Degradation on Soil Carbon Cycle， Microbial Functional Genes and Metabolism[J]. Acta Agrestia Sinica, 2026 , 34 (7) : 2341 -2354 . DOI: 10.11733/j.issn.1007-0435.2026.07.001

基金

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青海省中央引导地方科技发展资金项目 (2024ZY011); 青海省重点研发与转化计划科技成果转化专项项目 (2024-NK-138); 中央级公益性科研院所基本科研业务费青年探索项目 (2025YSKY-39)

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2026年第34卷第7期

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doi: 10.11733/j.issn.1007-0435.2026.07.001

接收时间：2026-02-09
首发时间：2026-09-23

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收稿日期：2026-02-09
修回日期：2026-03-30

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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