Colonization of Plant Growth-Promoting Rhizobacteria and its Effects on the Growth of Stipa Purpurea Under Different Temperature Conditions
YI Guo-yun1,2, CHENG Liang1,2, LI Juan1,2, WANG Xin1
Affiliations
1 Academy of Agriculture and Forestry, Qinghai University, Xining, Qinghai Province 810016, China; 2 Qinghai Provincial Key Laboratory of Agricultural Integrated Pest Management, Xining, Qinghai Province 810016, China
To screen plant growth-promoting rhizobacteria （PGPR） that are capable of stably colonizing the rhizosphere of Stipa purpurea， four PGPR strains previously isolated from this alpine grass were evaluated. Rifampicin-resistant derivatives were generated to monitor root-zone colonization dynamics， and the effects of bacterial inoculation on seed germination， seedling morphology， and physiological indices were assessed under the culture of 15℃， 20℃， and 25℃. Serratia proteamaculans GD-1-1 and Paenarthrobacter nitroguajacolicus YD-2-4 established stable populations in the S. purpurea rhizosphere， and the colonization amounts were 1.22×108 and 0.06×108 CFU·g-1 at 20 d， respectively. Dilutions of YD-2-4 （1×101 and 1×102 CFU·mL-1） significantly enhanced germination and seedling growth across 15℃-25℃， concurrently increasing antioxidant enzyme activities and lowering malondialdehyde content. In contrast， the stimulatory effect of GD-1-1 （1×102 and 1×103 CFU·mL-1） declined with temperature and disappeared at 15℃. These results indicated that YD-2-4 possessed robust colonization ability and broad temperature adaptability， making it an excellent candidate for the development of microbial inoculants for alpine grasslands.
YI Guo-yun, CHENG Liang, LI Juan, WANG Xin.
Colonization of Plant Growth-Promoting Rhizobacteria and its Effects on the Growth of Stipa Purpurea Under Different Temperature Conditions[J].
Acta Agrestia Sinica,
2026
, 34
(6)
: 2316
-2328
.
DOI: 10.11733/j.issn.1007-0435.2026.06.034
基金
收起
青海省自然科学基金项目项目-青年 (2024-ZJ-942)
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