Influences of AsA Priming on AsA-GSH Cycle in Mitochondria of Bothriochloa ischaemum under Salt Stress
DONG Qiu-li1,2, WANG Jing-ying1, MA Ru-yue1, CHEN Yi-lin1, LI Yin-lin1, XIA Fang-shan1
Affiliations
1 College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China; 2 College of Forestry, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China
To provide a theoretical basis for the breeding of salt-tolerant germplasm resources in Bothriochloa ischaemum， this experiment investigated the effects of ascorbic acid （AsA） priming on the AsA-GSH cycle in the embryonic mitochondria of B. ischaemum under salt stress. Seeds of ‘Taihang’ B. ischaemum were used as experimental materials， and were primed with 0.1 mmol·L-1 AsA solution for 12 h， then were germinated in NaCl and Na2SO4 solutions containing 30 mmol·L-1 Na+. The changes in AsA-GSH cycle enzymes activities and lipid peroxidation levels in their mitochondria of radicle and plumule were analyzed. The results showed that the activities of catalase （CAT）， glutathione transferase （GST）， dehydroascorbate reductase （DHAR） and monodehydroascorbate reductase （MDHAR） in the mitochondria of radicle and plumule of B. ischaemum were increased significantly after AsA priming （P<0.05）， while the activities of superoxide dismutase （SOD）， ascorbate peroxidase （APX） and glutathione peroxidases （GPX）， as well as the contents of malondialdehyde （MDA） and H2O2， were decreased significantly （P<0.05）. Under salt stress， APX and GPX were the key enzymes regulating salt tolerance of embryonic mitochondria in B. ischaemum， but CAT， MDHAR and DHAR were served as the core regulatory enzymes after AsA priming. The membership function analysis indicated that priming with 0.1 mmol·L-1 AsA had the best effect on enhancing the activities of mitochondrial AsA-GSH cycle enzymes in the radicle of B. ischaemum under 30 mmol·L-1 NaCl stress.
DONG Qiu-li, WANG Jing-ying, MA Ru-yue, CHEN Yi-lin, LI Yin-lin, XIA Fang-shan.
Influences of AsA Priming on AsA-GSH Cycle in Mitochondria of Bothriochloa ischaemum under Salt Stress[J].
Acta Agrestia Sinica,
2026
, 34
(6)
: 2055
-2063
.
DOI: 10.11733/j.issn.1007-0435.2026.06.009
基金
收起
山西农业大学“十四五”生物育种工程项目“乡土草种质资源挖掘与新品种选育 (YZGC134)
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