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In this study we cloned the EnWRKY26 gene from Elymus nutans Griseb ‘Ba Qing’ and conducted bioinformatics analysis， subcellular localization analysis， gene expression profiling， and transcription factor activity validation. The results indicated that EnWRKY26 was 996 bp in length， encoding 331 amino acids， with a molecular weight of 35 501.70 Da. The theoretical isoelectric point of the EnWRKY26 protein was 9.49， with an instability index of 53.78 and an average hydrophobicity of -0.652， indicating that it was an unstable hydrophilic protein. The entire protein sequence lacks transmembrane domains， and its secondary structure is predominantly composed of random coils. EnWRKY26 is most closely related to TraseCS7B02G418600 and TraseCS7D02G497700 in terms of phylogenetic relationship. Subcellular localization analysis revealed that EnWRKY26 was localized in the nucleus. Transcription factor activity validation confirmed that it possesses transcriptional activation activity. Under salt stress treatment， the relative expression level of EnWRKY26 showed an increasing trend. This study provides a theoretical basis for an in-depth understanding of the molecular mechanism by which EnWRKY26 regulates the response of Elymus nutans to salt stress., authors=WANG Jiao-feng¹, LIU Yu-cheng¹, YANG Shi-yu¹, HU Tian-ming¹, HAN Xiang-yan¹, YANG Pei-zhi¹, authorsList=WANG Jiao-feng, LIU Yu-cheng, YANG Shi-yu, HU Tian-ming, HAN Xiang-yan, YANG Pei-zhi, authorCompany=1 Department of Grassland Science, College of Grassland Agriculture, Northwest A& F University, Yangling, Shaanxi Province 712100, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575118414766615, articleId=1309575117991141909, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=垂穗披碱草EnWRKY26基因克隆及表达分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=WRKY转录因子广泛参与植物的生长发育过程，并在响应生物和非生物胁迫中发挥重要作用，本研究从‘巴青’垂穗披碱草（Elymus nutans Griseb ‘Ba Qing’）中成功克隆了EnWRKY26基因，并对其进行了生物信息学分析、亚细胞定位分析、基因表达分析和转录因子活性验证等。结果表明：EnWRKY26全长996 bp，共编码331个氨基酸，分子量为35，501.70 Da；EnWRKY26蛋白理论等电点为9.49，不稳定系数为53.78，疏水性平均值为-0.652，是一种不稳定亲水性蛋白，整个蛋白序列无跨膜区域，二级结构以无规卷曲为主；EnWRKY26与小麦TraseCS7B02G418600和小麦TraseCS7D02G497700亲缘关系最近；亚细胞定位分析显示EnWRKY26存在于细胞核中；转录因子活性验证表明其具有转录激活活性；在盐胁迫处理下，EnWRKY26相对表达量呈现升高趋势。本研究为深入解析EnWRKY26调控垂穗披碱草响应盐胁迫的分子机制提供理论依据。, authors=王娇凤¹, 刘昱成¹, 杨世裕¹, 呼天明¹, 韩祥艳¹, 杨培志¹, authorsList=王娇凤, 刘昱成, 杨世裕, 呼天明, 韩祥艳, 杨培志, authorCompany=1 西北农林科技大学草业与草原学院, 陕西 杨凌 712100, correspAuthors=null, authorNote=王娇凤(2001-),女,汉族,山西忻州人,硕士研究生,主要从事牧草逆境生物学研究,E-mail:wang_jiaofeng@126.com, correspAuthorsNote=null, 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