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2 Department of Mining Engineering, Shanxi Institute of Technology, Yangquan, Shanxi Province 045000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575078598238651, articleId=1309575077864235450, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=偏关苜蓿MsLAC17基因克隆及其对非生物胁迫的响应分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=漆酶（Laccase，LAC）作为铜蓝氧化酶蛋白家族的重要成员，在植物生长发育调控及非生物胁迫响应过程中扮演着关键角色。本研究选取山西地方优良种质‘偏关’苜蓿（Medicago sativa ‘Pianguan’）作为试验材料，采用PCR技术克隆获得MsLAC17基因，通过生物信息学手段对该基因进行分析，并利用荧光定量PCR技术，系统探究了MsLAC17基因表达的组织特异性，及其在不同非生物胁迫下的响应情况。结果显示：MsLAC17基因全长1782 bp，编码593个氨基酸，该蛋白的相对分子质量为66.26 kDa，理论等电点为10.05，属于亲水性蛋白，主要位于叶绿体和线粒体中。系统进化分析结果表明，‘偏关’苜蓿LAC17蛋白与蒺藜苜蓿（Medicago truncatula）的LAC17蛋白亲缘关系较近。荧光定量PCR检测发现MsLAC17基因在‘偏关’苜蓿茎中表达量最高，且对盐、干旱和ABA处理均有响应，推测该基因可能在调控‘偏关’苜蓿应对非生物胁迫的过程中发挥作用，本研究结果为进一步明晰MsLAC17基因的生物学功能奠定理论基础。, authors=杜欣蔚¹, 戎睿伟¹, 华怡雯¹, 刘怡¹, 陶佳丽^1,2, 周思懿¹, 许涛¹, 岑慧芳¹, 朱慧森¹, authorsList=杜欣蔚, 戎睿伟, 华怡雯, 刘怡, 陶佳丽, 周思懿, 许涛, 岑慧芳, 朱慧森, authorCompany=1 山西农业大学草业学院, 山西 太谷 030801;
2 山西工程技术学院矿业工程系, 山西 阳泉 045000, correspAuthors=null, authorNote=杜欣蔚(2000-),女,汉族,山东淄博人,硕士研究生,主要从事牧草种质资源与育种相关研究,E-mail:dxwxiamo@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=cfA5Wtq8/71/7+DDDh5U8Q==, pdfFileSize=8321694, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目 (32071872); 山西省中央引导地方科技发展资金项目 (YDZJSX2022B006); 现代种业发展项目 (sxzyk202501-7); 山西省重点研发计划课题 (202102140601006-3); 山西省基础研究计划 (202303021221101); 山西省来晋科研奖励 (SXBYKY2022118); 优秀博士启动项目 (2021BQ01,2023BQ03); 山西省研究生实践创新项目 (2024SJ133); 山西省研究生学术创新项目 (2025XS300))}, authors=null, keywords=[Keyword(id=1309575078946365885, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575077864235450, language=CN, orderNo=1, 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