Article(id=1309573304730608015, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.05.010, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1750953600000, receivedDateStr=2025-06-27, revisedDate=1760976000000, revisedDateStr=2025-10-21, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156206543, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156206543, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156206543, creator=13701087609, updateTime=1790156206543, updator=13701087609, issue=Issue{id=1309573278520406630, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='5', pageStart='1557', pageEnd='1962', issueExtLink='null', onlineDate='null', pubDate='1778774400000', pubDateStr='2026-05-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156200293, creator='13701087609', updateTime=1790156821973, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575886110814336, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575886115008641, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1664, endPage=1676, ext={EN=ArticleExt(id=1309573305078735249, articleId=1309573304730608015, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Comprehensive Evaluation of Salt Tolerance of different Alfalfa (Medicago sativa L.) Cultivars during Germination and Seedling Stages under Salt Stress, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To evaluate the differences in salt tolerance among different alfalfa (Medicago sativa L.) cultivarsunder salt stress conditions and screen high-quality salt-tolerant germplasm resources,this study used 25 alfalfa cultivars as experimental materials, with two treatments of salt stress (200 mmol·L-1 NaCl solution)and control (ultrapure water). Germination and growth parameters were measured during the germination period (eight indicators, such as germination energy and germination rate, etc.) and seedling stage (six indicators, such as plant height and branch number, etc.), and a comprehensive evaluation of salt tolerance was conducted using methods such as membership function value, comprehensive salt tolerance (D value), and cluster analyses. The results showed that salt stress significantly reduced all germination parameters, with the seed vigor index exhibiting the highest reduction rate (-92.26%), whereas the seedling dry weight was the least affected (-11.76%). Cluster analysis classified the 25 alfalfa cultivars into five salt tolerance grades at the germination stage: included 2 of the strongest salt-tolerant cultivars, 4 strong salt-tolerant cultivars, 5 moderate salt-tolerant cultivars, 10 weak salt-resistance cultivars, and 4 salt-sensitive cultivars. Further evaluation of salt tolerance of 12 representative alfalfa cultivars during the seedling stage revealed salt stress had extremely significant effects on the plant height, above-ground fresh weight, relative water content, and electrical conductivity of alfalfa (P<0.01), with significant differences between cultivars. By integrating the indicators of both germination and seedling stages, the three strongest salt-tolerant cultivars, namely ‘Xinmu No.4’‘Zhongmu No.4’, and ‘Bara520YQ’, were ultimately selected as important germplasm resources for salt-tolerant breeding and cultivation in saline-alkali soil regions., authors=SU Li-he1, HUANG Rong-zheng1, HU Tian-yu1, MA Chun-hui1, authorsList=SU Li-he, HUANG Rong-zheng, HU Tian-yu, MA Chun-hui, authorCompany=1 College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309573304999043472, articleId=1309573304730608015, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=盐胁迫下不同紫花苜蓿品种萌发期和苗期的耐盐性综合评价, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为评价不同紫花苜蓿(Medicago sativa)品种盐胁迫下的耐盐性差异,筛选优质耐盐种质资源。本研究以25份苜蓿品种为材料,设置200 mmol·L⁻¹ NaCl盐胁迫和超纯水对照处理,测定萌发期(发芽势、发芽率等8项)及苗期(株高、分枝数等6项)参数,采用隶属函数、综合耐盐值D及聚类分析进行综合评价。结果显示:盐胁迫显著降低所有萌发参数,种子活力指数降幅最大(-92.26%),幼苗干重受影响最小(-11.76%);聚类分析将萌发期25份材料划分为5个耐盐等级,含2份强耐盐、4份较强耐盐、5份中等耐盐、10份弱耐盐及4份盐敏感品种。对12份代表性品种苗期评价发现,盐胁迫对株高、地上鲜重、叶片相对含水量及电导率影响极显著(P<0.01),且品种间差异显著。整合萌发期与苗期指标,最终筛选出‘新牧4号’‘中苜4号’和‘Bara520YQ’3个强耐盐品种,可作为耐盐育种及盐碱地栽培的重要种质资源。, authors=苏力合1, 黄嵘峥1, 胡天宇1, 马春晖1, authorsList=苏力合, 黄嵘峥, 胡天宇, 马春晖, authorCompany=1 石河子大学动物科技学院, 新疆 石河子 832000, correspAuthors=null, authorNote=苏力合(1995-),女,蒙古族,新疆塔城人,博士,主要从事牧草生理及分子机制研究,E-mail:lihesu2025@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, 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Elucidating the molecular mechanisms mediating plant salt-stress responses[J]. The New Phytologist, 2018, 217(2):523-539 QUAMRUZZAMAN M, NURUZZAMAN MANIK S M N, LIVERMORE M, et al. Multidimensional screening and evaluation of morpho-physiological indices for salinity stress tolerance in wheat[J]. Journal of Agronomy and Crop Science, 2022, 208(4):454-471 MUNNS R, TESTER M. Mechanisms of salinity tolerance[J]. Annual Review of Plant Biology, 2008, 59:651-681 VAN ZELM E, ZHANG Y, TESTERINK C. Salt tolerance mechanisms of plants[J]. Annual Review of Plant Biology, 2020, 71:403-433 GYANAGOUDAR H S, HATIYA S T, GUHEY A, et al. A comprehensive approach for evaluating salinity stress tolerance in brinjal (Solanum melongena L.) germplasm using membership function value[J]. Physiologia Plantarum, 2024, 176(2):e14239 WU H, GUO J R, WANG C F, et al. An effective screening method and a reliable screening trait for salt tolerance of brassica napus at the germination stage[J]. Frontiers in Plant Science, 2019, 26(10):530 WANG N, WAN C, GAO S, et al. Evaluation of salt tolerance of 80 introduced alfalfa varieties during germination[J]. Agricultural Research in the Arid Areas, 2024, 42(2):17-25, 52 王宁, 万畅, 高山, 等. 80份紫花苜蓿引进品种萌发期耐盐性评价[J]. 干旱地区农业研究, 2024, 42(2):17-25, 52 WANG N, WAN C, GAO S, et al. Screening and evaluation of salt tolerance of 80 alfalfa varieties at the seedling stage[J]. Pratacultural Science, 2024, 41(3):684-699 王宁, 万畅, 高山, 等. 80份紫花苜蓿品种苗期耐盐性筛选与评价[J]. 草业科学, 2024, 41(3):684-699 CHOUDHARY A, KAUR N, SHARMA A, et al. Evaluation and screening of elite wheat germplasm for salinity stress at the seedling phase[J]. Physiologia Plantarum, 2021, 173(4):2207-2215 SU L H, ZHANG F F, WANG X Z, et al. Effects of snow cover on the yield and nutritional quality of alfalfa with different fall-dormancy levels[J]. Acta Agrestia Sinica, 2021, 29(2):356-363 苏力合, 张凡凡, 王旭哲, 等. 积雪覆盖对不同秋眠型紫花苜蓿产草量及营养品质的影响[J]. 草地学报, 2021, 29(2):356-363 SU L H, ZHANG F F, WANG X Z, et al. Effects of snow cover on physiological characteristics and cold resistance related gene expression of alfalfa varieties with different fall dormancy levels[J]. Chinese Journal of Grassland, 2021, 43(5):18-26 苏力合, 张凡凡, 王旭哲, 等. 覆雪对不同秋眠型紫花苜蓿生理特性及抗寒基因表达的影响[J]. 中国草地学报, 2021, 43(5):18-26 ZHU C C, SHI K, HE Q K, et al. Effects of mixed saline-alkali stress on physiology and gene expression of alfalfa seedlings[J]. Acta Agrestia Sinica, 2024, 32(4):1044-1054 朱晨晨, 史昆, 何沁坤, 等. 混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响[J]. 草地学报, 2024, 32(4):1044-1054 CHEN C J, MA L, BAO M F, et al. Identification and evaluation of drought resistance for 111 germplasm resources of alfalfa during germination stage[J]. Scientia Agricultura Sinica, 2025, 58(10):1896-1907 陈彩锦, 马琳, 包明芳, 等. 111份紫花苜蓿种质资源萌发期抗旱性鉴定评价[J]. 中国农业科学, 2025, 58(10):1896-1907 E J F, YANG S Q, ZHANG W F, et al. Effect of forage planting on improvement of saline soil under subsurface pipe in agro-pastoral intertwined zones[J]. Transactions of the Chinese Society for Agricultural Machinery, 2024, 55(9):402-410 鄂继芳, 杨树青, 张万锋, 等. 暗管条件下牧草种植对农牧交错区盐渍土的改土效应[J]. 农业机械学报, 2024, 55(9):402-410 LV Y Q, HU J, GAO D X, et al. Effects of forage cultivation and cattle manure application on nutrient and humus composition of saline alkali soil in the Songnen Plain[J]. Journal of Agricultural Resources and Environment, 2025, 42(1):169-176 吕悦齐, 胡娟, 高东雪, 等. 饲草种植及牛粪施用对松嫩平原盐碱土养分及腐殖质组成的影响[J]. 农业资源与环境学报, 2025, 42(1):169-176 SHI J Q, XIE N, CUI S Q, et al. Evaluation of salt tolerance in 16 alfalfa varieties during germination under different salt concentration stress[J]. Acta Agrestia Sinica, 2025, 33(2):472-480 石嘉琦, 谢楠, 崔素倩, 等. 不同盐浓度胁迫下16个紫花苜蓿品种萌发期耐盐性评价[J]. 草地学报, 2025, 33(2):472-480 ZHU K, LIU H J, FENG C L, et al. Comprehensive evaluation on the salt tolerance of seed germination of different alfalfa varieties under salt stress[J]. Acta Agrestia Sinica, 2023, 31(12):3724-3733 朱琨, 刘骅峻, 冯成龙, 等. 盐胁迫对不同苜蓿品种种子萌发的耐盐性综合评价[J]. 草地学报, 2023, 31(12):3724-3733 WU M M, XU J Y, NIE Z J, et al. Physiological, biochemical and transcriptomic insights into the mechanisms by which molybdenum mitigates cadmium toxicity in Triticum aestivum L[J]. Journal of Hazardous Materials, 2024, 472:134516 ZHANG X Y, LI S H, TANG T, et al. Comparison of morphological, physiological, and related-gene expression responses to saline-alkali stress in eight apple rootstock genotypes[J]. Scientia Horticulturae, 2022, 306:111455 WANG M, DING Y, WANG Q, et al. NaCl treatment on physio-biochemical metabolism and phenolics accumulation in barley seedlings[J]. Food chemistry, 2020, 331:127282 LI H, LIU L, HUANG Q, et al. Identification and screening of salt tolerance of cotton germplasm resources at germination stage[J]. Acta Agronomica Sinica, 2024, 50(5):1147-1157 李航, 刘丽, 黄乾, 等. 棉花种质资源萌发期耐盐性鉴定及筛选[J]. 作物学报, 2024, 50(5):1147-1157 WANG Y L, WEI L, LI N, et al. Comprehensive evaluation of salt tolerance of three forage seed varieties during germination[J]. Pratacultural Science, 2023, 40(12):3104-3113 王娅琳, 魏琳, 李娜, 等. 3种牧草种子萌发期耐盐性综合评价[J]. 草业科学, 2023, 40(12):3104-3113 YANG X J, SUN Q Y, ZHANG Z, et al. Comprehensive evaluation on salt tolerance of 61 alfalfa cultivars during seed germination[J]. Acta Agrestia Sinica, 2025, 33(4):1181-1191 姚兴洁, 孙庆赢, 张昭, 等. 61个品种紫花苜蓿种子萌发期耐盐性综合评价[J]. 草地学报, 2025, 33(4):1181-1191 ZHANG Z Y, LI X, WANG Y, et al. Salt tolerance evaluation and salt tolerance index screening of 59 alfalfa germplasm materials at seedling stage[J]. Acta Agrestia Sinica, 2020, 28(1):112-121 张则宇, 李雪, 王焱, 等. 59份苜蓿种质材料苗期耐盐性评价及耐盐指标筛选[J]. 草地学报, 2020, 28(1):112-121 WANG Y N, PENG X Y, HUA X Y, et al. Research progress on the physiological function of plant cotyledons[J]. Pratacultural Science, 2018, 35(12):2988-2997 王英男, 彭晓媛, 华晓雨, 等. 植物子叶生理功能的研究进展[J]. 草业科学, 2018, 35(12):2988-2997 ZHANG X P, DENG Y T, YANG R, et al. Evaluation of salt tolerance of forage oat germplasm resources at germination and seedling stages[J]. Acta Agrestia Sinica, 2025, 33(1):3741-3755 张晓澎, 邓焱天, 杨蕊, 等. 饲用燕麦种质资源萌发期及苗期耐盐性鉴定评价[J]. 草地学报, 2025, 33(1):3741-3755 YAN J H, LI X C, SONG W X, et al. Evaluation of salt tolerance of 57 Bromus inermis germplasm at the germination and seedling stages[J].https://kns.cnki.net/kcms/detail/62.1069.S.20250528.1345.002.html 闫聚辉, 李小聪, 宋文学, 等. 57份无芒雀麦种质萌发期与苗期耐盐性评价[J/OL]. https://kns.cnki.net/kcms/detail/62.1069.S.20250528.1345.002.html XIONG X, GUI W Y, LIU M H, et al. Evaluation of salt tolerance in different alfalfa varieties under uniform and non-uniform salt stress[J]. Acta Prataculturae Sinica, 2018, 27(9):67-76 熊雪, 桂维阳, 刘沫含, 等. 不同紫花苜蓿品种在均匀与不均匀盐胁迫下的耐盐性评价[J]. 草业学报, 2018, 27(9):67-76 LÜ Y Z, Y X J, ZHANG Z, et al. Evaluation of alkali tolerance and key indicators screening during seed germination of different alfalfa cultivars[J]. Acta Agrestia Sinica, 2025, 33(6):1852-1861 吕艳贞, 姚兴洁, 张昭, 等. 不同品种紫花苜蓿种子萌发期耐碱性评价与关键指标筛选[J]. 草地学报, 2025, 33(6):1852-1861)
Comprehensive Evaluation of Salt Tolerance of different Alfalfa (Medicago sativa L.) Cultivars during Germination and Seedling Stages under Salt Stress
SU Li-he1, HUANG Rong-zheng1, HU Tian-yu1, MA Chun-hui1
Affiliations
1 College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China
To evaluate the differences in salt tolerance among different alfalfa (Medicago sativa L.) cultivarsunder salt stress conditions and screen high-quality salt-tolerant germplasm resources,this study used 25 alfalfa cultivars as experimental materials, with two treatments of salt stress (200 mmol·L-1 NaCl solution)and control (ultrapure water). Germination and growth parameters were measured during the germination period (eight indicators, such as germination energy and germination rate, etc.) and seedling stage (six indicators, such as plant height and branch number, etc.), and a comprehensive evaluation of salt tolerance was conducted using methods such as membership function value, comprehensive salt tolerance (D value), and cluster analyses. The results showed that salt stress significantly reduced all germination parameters, with the seed vigor index exhibiting the highest reduction rate (-92.26%), whereas the seedling dry weight was the least affected (-11.76%). Cluster analysis classified the 25 alfalfa cultivars into five salt tolerance grades at the germination stage: included 2 of the strongest salt-tolerant cultivars, 4 strong salt-tolerant cultivars, 5 moderate salt-tolerant cultivars, 10 weak salt-resistance cultivars, and 4 salt-sensitive cultivars. Further evaluation of salt tolerance of 12 representative alfalfa cultivars during the seedling stage revealed salt stress had extremely significant effects on the plant height, above-ground fresh weight, relative water content, and electrical conductivity of alfalfa (P<0.01), with significant differences between cultivars. By integrating the indicators of both germination and seedling stages, the three strongest salt-tolerant cultivars, namely ‘Xinmu No.4’‘Zhongmu No.4’, and ‘Bara520YQ’, were ultimately selected as important germplasm resources for salt-tolerant breeding and cultivation in saline-alkali soil regions.
Key words
Medicago sativa L.
/
Seed germination
/
Seedling stages
/
Salt tolerance
/
Comprehensive evaluation
SU Li-he, HUANG Rong-zheng, HU Tian-yu, MA Chun-hui.
Comprehensive Evaluation of Salt Tolerance of different Alfalfa (Medicago sativa L.) Cultivars during Germination and Seedling Stages under Salt Stress[J].
Acta Agrestia Sinica,
2026
, 34
(5)
: 1664
-1676
.
DOI: 10.11733/j.issn.1007-0435.2026.05.010
基金
收起
国家牧草产业技术体系 (CARS-34)
参考文献
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文献
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参考文献引证文献
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YANG Y Q, GUO Y. Elucidating the molecular mechanisms mediating plant salt-stress responses[J]. The New Phytologist, 2018, 217(2):523-539 QUAMRUZZAMAN M, NURUZZAMAN MANIK S M N, LIVERMORE M, et al. Multidimensional screening and evaluation of morpho-physiological indices for salinity stress tolerance in wheat[J]. Journal of Agronomy and Crop Science, 2022, 208(4):454-471 MUNNS R, TESTER M. Mechanisms of salinity tolerance[J]. Annual Review of Plant Biology, 2008, 59:651-681 VAN ZELM E, ZHANG Y, TESTERINK C. Salt tolerance mechanisms of plants[J]. Annual Review of Plant Biology, 2020, 71:403-433 GYANAGOUDAR H S, HATIYA S T, GUHEY A, et al. A comprehensive approach for evaluating salinity stress tolerance in brinjal (Solanum melongena L.) germplasm using membership function value[J]. Physiologia Plantarum, 2024, 176(2):e14239 WU H, GUO J R, WANG C F, et al. An effective screening method and a reliable screening trait for salt tolerance of brassica napus at the germination stage[J]. Frontiers in Plant Science, 2019, 26(10):530 WANG N, WAN C, GAO S, et al. Evaluation of salt tolerance of 80 introduced alfalfa varieties during germination[J]. Agricultural Research in the Arid Areas, 2024, 42(2):17-25, 52 王宁, 万畅, 高山, 等. 80份紫花苜蓿引进品种萌发期耐盐性评价[J]. 干旱地区农业研究, 2024, 42(2):17-25, 52 WANG N, WAN C, GAO S, et al. Screening and evaluation of salt tolerance of 80 alfalfa varieties at the seedling stage[J]. Pratacultural Science, 2024, 41(3):684-699 王宁, 万畅, 高山, 等. 80份紫花苜蓿品种苗期耐盐性筛选与评价[J]. 草业科学, 2024, 41(3):684-699 CHOUDHARY A, KAUR N, SHARMA A, et al. Evaluation and screening of elite wheat germplasm for salinity stress at the seedling phase[J]. Physiologia Plantarum, 2021, 173(4):2207-2215 SU L H, ZHANG F F, WANG X Z, et al. Effects of snow cover on the yield and nutritional quality of alfalfa with different fall-dormancy levels[J]. Acta Agrestia Sinica, 2021, 29(2):356-363 苏力合, 张凡凡, 王旭哲, 等. 积雪覆盖对不同秋眠型紫花苜蓿产草量及营养品质的影响[J]. 草地学报, 2021, 29(2):356-363 SU L H, ZHANG F F, WANG X Z, et al. Effects of snow cover on physiological characteristics and cold resistance related gene expression of alfalfa varieties with different fall dormancy levels[J]. Chinese Journal of Grassland, 2021, 43(5):18-26 苏力合, 张凡凡, 王旭哲, 等. 覆雪对不同秋眠型紫花苜蓿生理特性及抗寒基因表达的影响[J]. 中国草地学报, 2021, 43(5):18-26 ZHU C C, SHI K, HE Q K, et al. Effects of mixed saline-alkali stress on physiology and gene expression of alfalfa seedlings[J]. Acta Agrestia Sinica, 2024, 32(4):1044-1054 朱晨晨, 史昆, 何沁坤, 等. 混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响[J]. 草地学报, 2024, 32(4):1044-1054 CHEN C J, MA L, BAO M F, et al. Identification and evaluation of drought resistance for 111 germplasm resources of alfalfa during germination stage[J]. Scientia Agricultura Sinica, 2025, 58(10):1896-1907 陈彩锦, 马琳, 包明芳, 等. 111份紫花苜蓿种质资源萌发期抗旱性鉴定评价[J]. 中国农业科学, 2025, 58(10):1896-1907 E J F, YANG S Q, ZHANG W F, et al. Effect of forage planting on improvement of saline soil under subsurface pipe in agro-pastoral intertwined zones[J]. Transactions of the Chinese Society for Agricultural Machinery, 2024, 55(9):402-410 鄂继芳, 杨树青, 张万锋, 等. 暗管条件下牧草种植对农牧交错区盐渍土的改土效应[J]. 农业机械学报, 2024, 55(9):402-410 LV Y Q, HU J, GAO D X, et al. Effects of forage cultivation and cattle manure application on nutrient and humus composition of saline alkali soil in the Songnen Plain[J]. Journal of Agricultural Resources and Environment, 2025, 42(1):169-176 吕悦齐, 胡娟, 高东雪, 等. 饲草种植及牛粪施用对松嫩平原盐碱土养分及腐殖质组成的影响[J]. 农业资源与环境学报, 2025, 42(1):169-176 SHI J Q, XIE N, CUI S Q, et al. Evaluation of salt tolerance in 16 alfalfa varieties during germination under different salt concentration stress[J]. Acta Agrestia Sinica, 2025, 33(2):472-480 石嘉琦, 谢楠, 崔素倩, 等. 不同盐浓度胁迫下16个紫花苜蓿品种萌发期耐盐性评价[J]. 草地学报, 2025, 33(2):472-480 ZHU K, LIU H J, FENG C L, et al. Comprehensive evaluation on the salt tolerance of seed germination of different alfalfa varieties under salt stress[J]. Acta Agrestia Sinica, 2023, 31(12):3724-3733 朱琨, 刘骅峻, 冯成龙, 等. 盐胁迫对不同苜蓿品种种子萌发的耐盐性综合评价[J]. 草地学报, 2023, 31(12):3724-3733 WU M M, XU J Y, NIE Z J, et al. Physiological, biochemical and transcriptomic insights into the mechanisms by which molybdenum mitigates cadmium toxicity in Triticum aestivum L[J]. Journal of Hazardous Materials, 2024, 472:134516 ZHANG X Y, LI S H, TANG T, et al. Comparison of morphological, physiological, and related-gene expression responses to saline-alkali stress in eight apple rootstock genotypes[J]. Scientia Horticulturae, 2022, 306:111455 WANG M, DING Y, WANG Q, et al. NaCl treatment on physio-biochemical metabolism and phenolics accumulation in barley seedlings[J]. Food chemistry, 2020, 331:127282 LI H, LIU L, HUANG Q, et al. Identification and screening of salt tolerance of cotton germplasm resources at germination stage[J]. Acta Agronomica Sinica, 2024, 50(5):1147-1157 李航, 刘丽, 黄乾, 等. 棉花种质资源萌发期耐盐性鉴定及筛选[J]. 作物学报, 2024, 50(5):1147-1157 WANG Y L, WEI L, LI N, et al. Comprehensive evaluation of salt tolerance of three forage seed varieties during germination[J]. Pratacultural Science, 2023, 40(12):3104-3113 王娅琳, 魏琳, 李娜, 等. 3种牧草种子萌发期耐盐性综合评价[J]. 草业科学, 2023, 40(12):3104-3113 YANG X J, SUN Q Y, ZHANG Z, et al. Comprehensive evaluation on salt tolerance of 61 alfalfa cultivars during seed germination[J]. Acta Agrestia Sinica, 2025, 33(4):1181-1191 姚兴洁, 孙庆赢, 张昭, 等. 61个品种紫花苜蓿种子萌发期耐盐性综合评价[J]. 草地学报, 2025, 33(4):1181-1191 ZHANG Z Y, LI X, WANG Y, et al. Salt tolerance evaluation and salt tolerance index screening of 59 alfalfa germplasm materials at seedling stage[J]. Acta Agrestia Sinica, 2020, 28(1):112-121 张则宇, 李雪, 王焱, 等. 59份苜蓿种质材料苗期耐盐性评价及耐盐指标筛选[J]. 草地学报, 2020, 28(1):112-121 WANG Y N, PENG X Y, HUA X Y, et al. Research progress on the physiological function of plant cotyledons[J]. Pratacultural Science, 2018, 35(12):2988-2997 王英男, 彭晓媛, 华晓雨, 等. 植物子叶生理功能的研究进展[J]. 草业科学, 2018, 35(12):2988-2997 ZHANG X P, DENG Y T, YANG R, et al. Evaluation of salt tolerance of forage oat germplasm resources at germination and seedling stages[J]. Acta Agrestia Sinica, 2025, 33(1):3741-3755 张晓澎, 邓焱天, 杨蕊, 等. 饲用燕麦种质资源萌发期及苗期耐盐性鉴定评价[J]. 草地学报, 2025, 33(1):3741-3755 YAN J H, LI X C, SONG W X, et al. Evaluation of salt tolerance of 57 Bromus inermis germplasm at the germination and seedling stages[J].https://kns.cnki.net/kcms/detail/62.1069.S.20250528.1345.002.html 闫聚辉, 李小聪, 宋文学, 等. 57份无芒雀麦种质萌发期与苗期耐盐性评价[J/OL]. https://kns.cnki.net/kcms/detail/62.1069.S.20250528.1345.002.html XIONG X, GUI W Y, LIU M H, et al. Evaluation of salt tolerance in different alfalfa varieties under uniform and non-uniform salt stress[J]. Acta Prataculturae Sinica, 2018, 27(9):67-76 熊雪, 桂维阳, 刘沫含, 等. 不同紫花苜蓿品种在均匀与不均匀盐胁迫下的耐盐性评价[J]. 草业学报, 2018, 27(9):67-76 LÜ Y Z, Y X J, ZHANG Z, et al. Evaluation of alkali tolerance and key indicators screening during seed germination of different alfalfa cultivars[J]. Acta Agrestia Sinica, 2025, 33(6):1852-1861 吕艳贞, 姚兴洁, 张昭, 等. 不同品种紫花苜蓿种子萌发期耐碱性评价与关键指标筛选[J]. 草地学报, 2025, 33(6):1852-1861