Article(id=1309573251349700731, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.04.005, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1753804800000, receivedDateStr=2025-07-30, revisedDate=1756915200000, revisedDateStr=2025-09-04, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156193816, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156193816, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156193816, creator=13701087609, updateTime=1790156193816, updator=13701087609, issue=Issue{id=1309573247344144835, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='4', pageStart='1137', pageEnd='1556', issueExtLink='null', onlineDate='null', pubDate='1776182400000', pubDateStr='2026-04-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156192860, creator='13701087609', updateTime=1790156800047, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575794117144683, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575794117144684, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1181, endPage=1195, ext={EN=ArticleExt(id=1309573251769131134, articleId=1309573251349700731, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Screening of Salt-Tolerant and Growth-Promoting Strains of Cyperus esculentus Rhizosphere in Sandy Areas of South Xinjiang, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To screen salt-tolerant and growth-promoting bacterial strains adapted to the extreme environment of Southern Xinjiang from the rhizosphere soil of Cyperus esculentus L. and enrich the strain resource bank for saline-alkali sandy soils in Xinjiang, this study employed the targeted screening medium culture method, shake flask culture method, and 16S rDNA identification method to isolate salt-tolerant and growth-promoting strains, determine their growth-promoting characteristics, and finally further verify the growth-promoting effects of these strains through Cyperus esculentus seed germination tests and potted seedling experiments. A total of 10 salt-tolerant and growth-promoting strains were obtained, which belonged to the genera Bacillus, Vreelandella, Exiguobacterium, and Bisbaumannia, with their salt tolerance thresholds ranging from 4% to 8% NaCl. Among these strains, strain Y-38 (Exiguobacterium acetylicum) exhibited prominent inorganic phosphorus-solubilizing ability (0.65 mg·L-1), strain Y-35 (Exiguobacterium mexicanum) showed the highest production of indole-3-acetic acid (IAA) (64.35 mg·L-1), and strain Y-46 (Bisbaumannia pacifica) could tolerate 8% NaCl. In the seed germination experiment, the germination rate of the Y-38 treatment group reached 82.00%, which was significantly 26.89% higher than the control group (P<0.05); in the potted experiment, growth indicators of the Y-38 treatment group, including plant height (39.17 cm), leaf width (5.22 mm), and stem diameter (5.52 mm), were all significantly higher than those of the Y-46 and Y-35 treatment groups (P<0.05). The comprehensive ranking of the growth-promoting effects of the three strains was Y-38>Y-46>Y-35. Ultimately, the high salt tolerance and growth-promoting function of strain Y-38 (Exiguobacterium acetylicum) was confirmed, providing a bacterial strain resources for the improvement of saline-alkali soils., authors=ZHANG Ya-ning1, MA Zheng-dong1, CHEN Ying1, TONG Xin1, ZHANG Sheng-jie1, JIA Shu-an1, WANG Xu-zhe1, MA Chun-hui1, authorsList=ZHANG Ya-ning, MA Zheng-dong, CHEN Ying, TONG Xin, ZHANG Sheng-jie, JIA Shu-an, WANG Xu-zhe, MA Chun-hui, authorCompany=1 College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309573251655884924, articleId=1309573251349700731, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=南疆沙区油莎豆根际耐盐促生菌株的筛选, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为从油莎豆(Cyperus esculentus L.)根际土壤中筛选适应南疆极端环境的耐盐促生菌株,丰富新疆盐碱沙土地菌株资源库,本研究采用定向筛选培养基培养法、摇瓶培养法和16S rDNA鉴定法,对耐盐促生菌进行筛选,并测定其促生特性。最终通过油莎豆种子萌发和盆栽幼苗试验,进一步验证其促生效果。本研究共获得10株耐盐促生菌,分属于芽孢杆菌属(Bacillus)、弗里兰氏菌属(Vreelandella)、微小杆菌属(Exiguobacterium)和比斯鲍曼氏菌属(Bisbaumannia),耐NaCl阈值范围为4%~8%。其中菌株Y-38(Exiguobacterium acetylicum)溶无机磷能力突出(0.65 mg·L⁻¹),Y-35(Exiguobacterium mexicanum)产吲哚-3-乙酸(Indole-3-acetic acid,IAA)量最高(64.35 mg·L⁻¹),Y-46(Bisbaumannia pacifica)耐受8%NaCl。种子萌发试验中,Y-38处理组发芽率达82.00%,较对照显著提高26.89%(P<0.05);盆栽试验中Y-38处理组的株高(39.17cm)、叶宽(5.22mm)、茎粗(5.52mm)等指标均显著高于Y-46和Y-35处理组(P<0.05)。三株菌的促生效果通过综合排序为:Y-38>Y-46>Y-35,最终确定了Y-38(乙酰微小杆菌,Exiguobacterium acetylicum)的高耐盐性与促生功能,为盐碱土壤改良提供了菌种资源。, authors=张亚宁1, 马正东1, 陈莹1, 佟鑫1, 张盛洁1, 贾舒安1, 王旭哲1, 马春晖1, authorsList=张亚宁, 马正东, 陈莹, 佟鑫, 张盛洁, 贾舒安, 王旭哲, 马春晖, authorCompany=1 石河子大学动物科技学院, 新疆 石河子 832000, correspAuthors=null, authorNote=张亚宁(2004-),男,汉族,河北邢台人,本科生,主要从事牧草生产与加工研究,E-mail:2357678055@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=1WTzcQxGFsq0C+cx8xExqw==, pdfFileSize=11801618, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=兵团科技计划项目 (2024DB023); 石河子大学青年拔尖计划项目 (BJZK202406); 国家自然科学基金项目 (32260856); 国家牧草产业技术体系项目 (CARS))}, authors=null, keywords=[Keyword(id=1309573251899154559, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573251349700731, language=CN, orderNo=1, keyword=油莎豆), Keyword(id=1309573251962069120, tenantId=1146029695717560320, 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南疆沙区油莎豆根际耐盐促生菌株的筛选
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草地学报 | 研究论文 2026,34(4): 1181-1195
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草地学报 |研究论文 2026 , 34 (4) : 1181 -1195
南疆沙区油莎豆根际耐盐促生菌株的筛选
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张亚宁1, 马正东1, 陈莹1, 佟鑫1, 张盛洁1, 贾舒安1, 王旭哲1, 马春晖1
作者信息
    1 石河子大学动物科技学院, 新疆 石河子 832000
作者简介:
张亚宁(2004-),男,汉族,河北邢台人,本科生,主要从事牧草生产与加工研究,E-mail:2357678055@qq.com
Screening of Salt-Tolerant and Growth-Promoting Strains of Cyperus esculentus Rhizosphere in Sandy Areas of South Xinjiang
  • ZHANG Ya-ning1, MA Zheng-dong1, CHEN Ying1, TONG Xin1, ZHANG Sheng-jie1, JIA Shu-an1, WANG Xu-zhe1, MA Chun-hui1
  • Affiliations
      1 College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China
    doi: 10.11733/j.issn.1007-0435.2026.04.005
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    为从油莎豆(Cyperus esculentus L.)根际土壤中筛选适应南疆极端环境的耐盐促生菌株,丰富新疆盐碱沙土地菌株资源库,本研究采用定向筛选培养基培养法、摇瓶培养法和16S rDNA鉴定法,对耐盐促生菌进行筛选,并测定其促生特性。最终通过油莎豆种子萌发和盆栽幼苗试验,进一步验证其促生效果。本研究共获得10株耐盐促生菌,分属于芽孢杆菌属(Bacillus)、弗里兰氏菌属(Vreelandella)、微小杆菌属(Exiguobacterium)和比斯鲍曼氏菌属(Bisbaumannia),耐NaCl阈值范围为4%~8%。其中菌株Y-38(Exiguobacterium acetylicum)溶无机磷能力突出(0.65 mg·L⁻¹),Y-35(Exiguobacterium mexicanum)产吲哚-3-乙酸(Indole-3-acetic acid,IAA)量最高(64.35 mg·L⁻¹),Y-46(Bisbaumannia pacifica)耐受8%NaCl。种子萌发试验中,Y-38处理组发芽率达82.00%,较对照显著提高26.89%(P<0.05);盆栽试验中Y-38处理组的株高(39.17cm)、叶宽(5.22mm)、茎粗(5.52mm)等指标均显著高于Y-46和Y-35处理组(P<0.05)。三株菌的促生效果通过综合排序为:Y-38>Y-46>Y-35,最终确定了Y-38(乙酰微小杆菌,Exiguobacterium acetylicum)的高耐盐性与促生功能,为盐碱土壤改良提供了菌种资源。
    油莎豆  /  植物根际促生菌  /  耐盐菌  /  分离鉴定
    To screen salt-tolerant and growth-promoting bacterial strains adapted to the extreme environment of Southern Xinjiang from the rhizosphere soil of Cyperus esculentus L. and enrich the strain resource bank for saline-alkali sandy soils in Xinjiang, this study employed the targeted screening medium culture method, shake flask culture method, and 16S rDNA identification method to isolate salt-tolerant and growth-promoting strains, determine their growth-promoting characteristics, and finally further verify the growth-promoting effects of these strains through Cyperus esculentus seed germination tests and potted seedling experiments. A total of 10 salt-tolerant and growth-promoting strains were obtained, which belonged to the genera Bacillus, Vreelandella, Exiguobacterium, and Bisbaumannia, with their salt tolerance thresholds ranging from 4% to 8% NaCl. Among these strains, strain Y-38 (Exiguobacterium acetylicum) exhibited prominent inorganic phosphorus-solubilizing ability (0.65 mg·L-1), strain Y-35 (Exiguobacterium mexicanum) showed the highest production of indole-3-acetic acid (IAA) (64.35 mg·L-1), and strain Y-46 (Bisbaumannia pacifica) could tolerate 8% NaCl. In the seed germination experiment, the germination rate of the Y-38 treatment group reached 82.00%, which was significantly 26.89% higher than the control group (P<0.05); in the potted experiment, growth indicators of the Y-38 treatment group, including plant height (39.17 cm), leaf width (5.22 mm), and stem diameter (5.52 mm), were all significantly higher than those of the Y-46 and Y-35 treatment groups (P<0.05). The comprehensive ranking of the growth-promoting effects of the three strains was Y-38>Y-46>Y-35. Ultimately, the high salt tolerance and growth-promoting function of strain Y-38 (Exiguobacterium acetylicum) was confirmed, providing a bacterial strain resources for the improvement of saline-alkali soils.
    Cyperus esculentus L.  /  Rhizosphere growth-promoting bacteria  /  Salt-tolerant bacteria  /  Isolation and identification
    张亚宁, 马正东, 陈莹, 佟鑫, 张盛洁, 贾舒安, 王旭哲, 马春晖. 南疆沙区油莎豆根际耐盐促生菌株的筛选. 草地学报, 2026 , 34 (4) : 1181 -1195 . DOI: 10.11733/j.issn.1007-0435.2026.04.005
    ZHANG Ya-ning, MA Zheng-dong, CHEN Ying, TONG Xin, ZHANG Sheng-jie, JIA Shu-an, WANG Xu-zhe, MA Chun-hui. Screening of Salt-Tolerant and Growth-Promoting Strains of Cyperus esculentus Rhizosphere in Sandy Areas of South Xinjiang[J]. Acta Agrestia Sinica, 2026 , 34 (4) : 1181 -1195 . DOI: 10.11733/j.issn.1007-0435.2026.04.005

      兵团科技计划项目 (2024DB023); 石河子大学青年拔尖计划项目 (BJZK202406); 国家自然科学基金项目 (32260856); 国家牧草产业技术体系项目 (CARS)

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    Number of
    species
    占总种数比例
    Percentage of
    total species (%)
    属
    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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