Article(id=1309569854936544153, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.03.027, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1744819200000, receivedDateStr=2025-04-17, revisedDate=1751990400000, revisedDateStr=2025-07-09, acceptedDate=null, acceptedDateStr=null, onlineDate=1790155384048, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790155384048, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790155384048, creator=13701087609, updateTime=1790155384048, updator=13701087609, issue=Issue{id=1309569821424051089, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='3', pageStart='745', pageEnd='1136', issueExtLink='null', onlineDate='null', pubDate='1773504000000', pubDateStr='2026-03-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790155376058, creator='13701087609', updateTime=1790156773348, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575682133422151, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575682133422152, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1041, endPage=1052, ext={EN=ArticleExt(id=1309569855309837212, articleId=1309569854936544153, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Biochar and Nitrogen Co-Application on Vegetation and Soil Restoration in Bare-Land Artificial Restoration Grassland, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To investigate the effects of biochar combined with nitrogen fertilizer on vegetation， soil physicochemical properties， and extracellular enzyme activities in artificially restored bare-land grasslands in the Qilian Mountains， a randomized block design was conducted in 2021. The treatments included a basal fertilizer （biochar 0.5 t·hm^-2 + organic fertilizer 1.5 t·hm^-2） with nitrogen additions of 50， 100， 150， and 200 kg·hm^-2 （CN₁， CN₂， CN₃， CN₄）， as well as CN₀ （basal fertilizer only）， CN₅ （200 kg·hm^-2 nitrogen without basal fertilizer）， and CK （no fertilization）， totaling seven treatments. The results showed that biochar-nitrogen application significantly increased aboveground biomass （AB）， belowground biomass （BB）， and root/shoot ratio （R/S） （P<0.05）. Specifically， CN₃ significantly enhanced soil water content （SWC）， total nitrogen （TN）， soil organic carbon （SOC）， and nitrate nitrogen （NO₃^--N）， while CN₃ and CN₄ significantly promoted the activities of β-1，4-acetylglucosaminidase （NAG）， leucine aminopeptidase （LAP）， and cellobiose hydrolase （CBH） （P<0.05）. Piecewise structural equation modeling （SEM） indicated that biochar-nitrogen application improved vegetation performance and soil fertility by stimulating extracellular enzyme activities and nutrient supply. In conclusion， CN₃ （basal fertilizer + 150 kg·hm^-2 nitrogen， C∶N≈12∶1） showed the optimal effect， providing a theoretical reference for the restoration of bare-land artificial grassland in alpine regions., authors=ZHENG Wen-xian¹, LI Shi-xiong^1,2,3, ZHAO Wen¹, SONG Jiang-qin¹, HUO Jiu-yan¹, SUI Qi-qi¹, XU Hai-feng¹, YIN Ya-li^1,2, authorsList=ZHENG Wen-xian, LI Shi-xiong, ZHAO Wen, SONG Jiang-qin, HUO Jiu-yan, SUI Qi-qi, XU Hai-feng, YIN Ya-li, authorCompany=1 Academy of Animal Science and Veterinary Medicine Qinghai University, Xining, Qinghai Province 810016, China;
2 Key Laboratory of Alpine Grassland Ecosystem in the Three-River-Source, Ministry of Education, Qinghai University, Xining, Qinghai Province 810016, China;
3 Qinghai Provincial Key Laboratory of Adaptive Management on Alpine Grassland, Xining, Qinghai Province 810016, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569855230145434, articleId=1309569854936544153, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=炭氮配施对黑土滩人工草地植被与土壤的修复研究, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为探究祁连山区生物炭配施氮肥对黑土滩人工草地植被、土壤理化性质及胞外酶活性的影响，本研究于2021年采用随机区组设计开展试验。试验设基肥（生物炭0.5 t·hm⁻²+有机肥1.5 t·hm⁻²），并在此基础上配施不同梯度的氮肥，分别为50，100，150，200 kg·hm⁻²（对应处理CN₁~CN₄），并设CN₀（基肥）、CN₅（氮肥200 kg·hm⁻²）和CK（不施肥），共7个处理。结果表明，氮配施显著提高植被地上、地下生物量和根冠比（P<0.05）。CN₃处理显著提高土壤含水量、全氮、有机碳和硝态氮含量。CN₃和CN₄处理显著增加β-1，4-乙酰氨基葡萄糖苷酶、亮氨酸氨基肽酶和纤维素二糖水解酶活性（P<0.05）。Piecewise SEM（Structural equation modeling）模型分析结果表明，炭氮配施通过提高胞外酶活性、促进养分供应，改善植被生长状况和土壤肥力。综上，CN₃（基肥+氮肥150 kg·hm⁻²，C∶N≈12∶1）对黑土滩人工草地植被与土壤的修复效果最佳，本研究可为黑土滩草地修复提供理论参考。, authors=郑文贤¹, 李世雄^1,2,3, 赵文¹, 宋江琴¹, 火久艳¹, 随奇奇¹, 徐海峰¹, 尹亚丽^1,2, authorsList=郑文贤, 李世雄, 赵文, 宋江琴, 火久艳, 随奇奇, 徐海峰, 尹亚丽, authorCompany=1 青海大学畜牧兽医科学院, 青海 西宁 810016;
2 三江源区高寒草地生态教育部重点实验室(青海大学), 青海 西宁 810016;
3 青海省高寒草地适应性管理重点实验室, 青海 西宁 810016, correspAuthors=null, authorNote=郑文贤（1997-），男，汉族，甘肃古浪人，博士研究生，主要从事草地生态与环境保护研究，E-mail：2874009886@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=DQ2Bi/0OaZCLhZxsZwGfiA==, pdfFileSize=3424905, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目 (32260357); 青海省科技项目 (2023-ZJ-925M))}, authors=null, keywords=[Keyword(id=1309569855444054941, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=CN, orderNo=1, keyword=生物炭与氮肥), Keyword(id=1309569855511163806, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=CN, orderNo=2, keyword=黑土滩人工草地), Keyword(id=1309569855595049887, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=CN, orderNo=3, keyword=土壤理化性质), Keyword(id=1309569857293743008, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=CN, orderNo=4, keyword=植被生物量), Keyword(id=1309569857369240482, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=CN, orderNo=5, keyword=胞外酶活性), Keyword(id=1309569857474098083, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=EN, orderNo=1, keyword=Biochar and nitrogen fertilizer), Keyword(id=1309569857541206948, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=EN, orderNo=2, keyword=Bare-land artificial restoration grassland), Keyword(id=1309569857616704421, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=EN, orderNo=3, keyword=Soil physicochemical properties), Keyword(id=1309569857692201895, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=EN, orderNo=4, keyword=Vegetation biomass), Keyword(id=1309569857771893672, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569854936544153, language=EN, orderNo=5, keyword=Extracellular enzyme activity)], refs=null, funds=null, companyList=null, figs=null, attaches=null, journal=Journal(id=1308395841968504927, delFlag=0, nameCn=草地学报, nameEn=Acta Agrestia Sinica, nameHistory1=null, nameHistory2=null, issn=1007-0435, eissn=null, cn=11-3362/S, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=gCdi9sXCX82eLR6S1d4VLg==, journalPrice=null, startedYear=null, abbrevIsoEn=Acta Agrestia Sinica, journalRemark=null, publicationField=null, createdTime=1789875477545, updatedTime=1789876241473, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=C, firstLetterEn=C, subjectCode=Agriculture, subjectName=null, subjectCodeEn=Agriculture, subjectNameEn=null, picCn=gCdi9sXCX82eLR6S1d4VLg==, picEn=4dKqEDfsaGuyf+bpm/3gfw==, jcr=null, cjcr=null, exts=[JournalExt(id=1308399046192812446, language=CN, name=草地学报, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789876241491, updatedTime=1789876241491, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/authorLogOn.action, submissionEditorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/editorLogOn.action, submissionReviewUrl=https://manu40.magtech.com.cn/JournalX_cdxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1308399046268309919, language=EN, name=Acta Agrestia Sinica, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789876241509, updatedTime=1789876241509, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/authorLogOn.action, submissionEditorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/editorLogOn.action, submissionReviewUrl=https://manu40.magtech.com.cn/JournalX_cdxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1308397196074385509, websiteList=[Website(id=1308429426430071695, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1308397196074385509, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/cdxb/CN, language=CN, createTime=1789883484704, createBy=18614031015, updateTime=1789883745058, updateBy=18614031015, name=草地学报-中文, tplId=1146099689490845704, title=草地学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1308430669353340972, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=articleTextType, value=kx, createTime=1789883781040, updateTime=1789883781040, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669328175145, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=banner, value=null, createTime=1789883781034, updateTime=1789883781034, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669382701103, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=grayFlag, value=0, createTime=1789883781047, updateTime=1789883781047, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669319786536, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=logo, value=https://castjournals.cast.org.cn/joweb/cdxb/CN/file/pic?fileId=BZ28lIoKdB54MmA0z6K66g==, createTime=1789883781032, updateTime=1789883781032, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669395284017, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=minRunFlag, value=0, createTime=1789883781050, updateTime=1789883781050, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669344952363, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/cdxb/CN/file/pic, createTime=1789883781038, updateTime=1789883781038, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669391089712, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=silenceFlag, value=0, createTime=1789883781049, updateTime=1789883781049, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669336563754, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1789883781036, updateTime=1789883781036, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669361729581, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=themeColor, value=null, createTime=1789883781042, updateTime=1789883781042, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669370118190, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=themeStyle, value=null, createTime=1789883781044, updateTime=1789883781044, creator=18614031015, updator=18614031015)]), Website(id=1308429426501374870, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1308397196074385509, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/cdxb/EN, language=EN, createTime=1789883484721, createBy=18614031015, updateTime=1789883716139, updateBy=18614031015, name=草地学报-英文, tplId=1146101810881728533, title=Acta Agrestia Sinica, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1308430635979264030, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=articleTextType, value=kx, createTime=1789883773083, updateTime=1789883773083, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635954098203, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=banner, value=null, createTime=1789883773077, updateTime=1789883773077, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636000235553, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=grayFlag, value=0, createTime=1789883773088, updateTime=1789883773088, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635945709594, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=logo, value=https://castjournals.cast.org.cn/joweb/cdxb/EN/file/pic?fileId=BZ28lIoKdB54MmA0z6K66g==, createTime=1789883773075, updateTime=1789883773075, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636012818467, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=minRunFlag, value=0, createTime=1789883773091, updateTime=1789883773091, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635970875421, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/cdxb/EN/file/pic, createTime=1789883773081, updateTime=1789883773081, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636004429858, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=silenceFlag, value=0, createTime=1789883773089, updateTime=1789883773089, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635962486812, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1789883773079, updateTime=1789883773079, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635987652639, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=themeColor, value=null, createTime=1789883773085, updateTime=1789883773085, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635991846944, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=themeStyle, value=null, createTime=1789883773086, updateTime=1789883773086, creator=18614031015, updator=18614031015)])], journalTitle=草地学报, weixinUrl=null, journalUrl=https://manu40.magtech.com.cn/Jweb_cdxb, iacademicId=null, status=1, seqNo=null, journalTitleEn=Acta Agrestia Sinica, journalPhotoCn=gCdi9sXCX82eLR6S1d4VLg==, journalPhotoEn=4dKqEDfsaGuyf+bpm/3gfw==, journalFirstLetter=C, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/10.11733/j.issn.1007-0435.2026.03.027, detailUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/10.11733/j.issn.1007-0435.2026.03.027, pdfUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/PDF/10.11733/j.issn.1007-0435.2026.03.027, pdfUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/PDF/10.11733/j.issn.1007-0435.2026.03.027, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1773504000000, fullTextJson=null, articleText=null, reference=DU Z Y，CONG N. Responses of vegetation and soil characterisitics to degraded grassland under different degrees on the Qinghai-Tibet Plateau[J]. Acta Ecologica Sinica，2024，44（6）：2504-2516杜志勇，丛楠.植被与土壤特征对青藏高原不同程度退化草地的响应[J]. 生态学报，2024，44（6）：2504-2516
ZHANG J G，WANG L D，YAO T，et al. Characteristics of plant community and soil nutrient of different degraded grasslands in east Qilian Mountains[J]. Journal of Soil and Water Conservation，2019，33（1）：227-233张建贵，王理德，姚拓，等. 东祁连山不同退化草地植物群落特征与土壤养分特性[J]. 水土保持学报，2019，33（1）：227-233
LIN L，ZHANG D G，CAO G M，et al. Responses of soil nutrient traits to grazing intensities in alpine Kobresia meadows[J]. Acta Ecologica Sinica，2016，36（15）：4664-4671林丽，张德罡，曹广民，等. 放牧强度对高寒嵩草草甸土壤养分特性的影响[J]. 生态学报，2016，36（15）：4664-4671
SHANG Z H，DONG Q M，SHI J J，et al. Research progress in recent ten years of ecological restoration for “black soil land” degraded grassland on Tibetan Plateau—concurrently discuss of ecological restoration in Sang jiangyuan region[J]. Acta Agrestia Sinica，2018，26（1）：1-21尚占环，董全民，施建军，等. 青藏高原“黑土滩”退化草地及其生态恢复近10年研究进展——兼论三江源生态恢复问题[J]. 草地学报，2018，26（1）：1-21
DUAN C W，LI X L，MA P P，et al. Effect of artificial restoration measure on soil nutrients and enzyme activities of degraded alpine meadow[J]. Acta Agriculturae Boreali-occidentalis Sinica，2022，31（4）：431-440段成伟，李希来，马盼盼，等. 人工修复措施对退化高寒草甸土壤养分及酶活性的影响[J]. 西北农业学报，2022，31（4）：431-440
LI X Y，LI Y H，QIN W P，et al. Impacts of artificial grasslands on soil organic carbon fractions in a degraded alpine meadow of the Three-River Source Region[J]. Chinese Journal of Ecology，2024，43（1）：50-56李晓燕，李永慧，秦文萍，等. 人工草地建植对三江源区退化高寒草甸土壤有机碳组分的影响[J]. 生态学杂志，2024，43（1）：50-56
XIA H. Effect of nitrogen fertilizer combined with biochar on improving fertility and nitrogen utilization efficiency of acid soil and its microbiological mechanism[D]. Wuhan：Huazhong Agricultural University，2023：14-21夏浩. 生物炭与氮肥配施提升酸性土壤肥力和氮素利用率的效应及其微生物学机制[D]. 武汉：华中农业大学，2023：14-21
ZHANG T，CHEN X P，ZHAO J X，et al. Effects of carbon and nitrogen addition on the plant species diversity and biomass of typical alpine meadow in Tibet of China[J]. Ecology and Environmental Sciences，2015，24（10）：1604-1610张涛，陈晓鹏，赵景学，等. 碳、氮添加对高寒草甸植物群落物种多样性和生物量的影响[J]. 生态环境学报，2015，24（10）：1604-1610
DUAN J J，GUO Q B，XU B，et al. Effects of biochar application with reduced nitrogen fertilizer on rice yield and nutrient utilization[J]. Journal of Soil and Water Conservation，2022，36（6）：298-308段建军，郭琴波，徐彬，等. 氮肥减量施生物炭对水稻产量和养分利用的影响[J]. 水土保持学报，2022，36（6）：298-308
HU J Y，YANG H J，LIU D J，et al. Effects of brewery-waste derived biochar on soil physicochemical properties and crop yield[J]. Journal of Plant Nutrition and Fertilizers，2022，28（9）：1664-1672胡京钰，杨红军，刘大军，等. 酒糟生物炭与化肥配施对土壤理化特性及作物产量的影响[J]. 植物营养与肥料学报，2022，28（9）：1664-1672
GE X G，CAO Y H，ZHOU B Z，et al. Combined application of biochar and N increased temperature sensitivity of soil respiration but still decreased the soil CO₂ emissions in moso bamboo plantations[J]. Science of the Total Environment，2020，730：139003
MENG F H. Influence mechanism of soil physicochemical property and yield and nitrogen efficiency of spring corn by combined application of biochar with nitrogen fertilizer[D]. Hohhot：Inner Mongolia Agricultural University，2018：2-10孟繁昊. 生物炭配施氮肥对土壤理化性质及春玉米产量和氮效率的影响机制[D]. 呼和浩特：内蒙古农业大学，2018：2-10
ZHANG C R，YANG Y L，CHENG Q G，et al. Effects of different tillages on soil microbial community structure and enzyme activity in Mollisols of China[J]. Soils and Crops，2020，9（4）：335-347张常仁，杨雅丽，程全国，等. 不同耕作模式对东北黑土微生物群落结构和酶活性的影响[J]. 土壤与作物，2020，9（4）：335-347
QIN J P，MA Y S，LI S X，et al. Effects of grazing intensity in spring on forage growth in Poa pratensis L. cv. Qinghai artificial grassland in Qilian mountains[J]. Journal of Qinghai University，2019，37（4）：1-6秦金萍，马玉寿，李世雄，等. 春季放牧强度对祁连山区青海草地早熟禾人工草地牧草生长的影响[J]. 青海大学学报，2019，37（4）：1-6
BAI S H，OMIDVAR N，GALLART M，et al. Combined effects of biochar and fertilizer applications on yield：a review and meta-analysis[J]. Science of the Total Environment，2022，808：152073
LI H R，ZHU Y，TIAN J H，et al. Effects of carbon and nitrogen additions on soil organic C，N，P contents and their catalyzed enzyme activities in a grassland[J]. Chinese Journal of Applied Ecology，2018，29（8）：2470-2476李焕茹，朱莹，田纪辉，等. 碳氮添加对草地土壤有机碳氮磷含量及相关酶活性的影响[J]. 应用生态学报，2018，29（8）：2470-2476
TIAN J H，WEI K，CONDRON L M，et al. Impact of land use and nutrient addition on phosphatase activities and their relationships with organic phosphorus turnover in semi-arid grassland soils[J]. Biology and Fertility of Soils，2016，52（5）：675-683
XU L L，WANG Q B，ZHANG X Y，et al. Effects of applying different kind fertilizers on enzyme activities related to carbon nitrogen and phosphorus cycles in reddish paddy soil[J]. The Journal of Applied Ecology，2013，24（4）：909-914
ZHANG L Y，XIANG Y Z，JING Y M，et al. Biochar amendment effects on the activities of soil carbon， nitrogen and phosphorus hydrolytic enzymes：a meta-analysis[J]. Environmental Science and Pollution Research，2019，26（22）：22990-23001
BAO S D. Soil agrochemical analysis[M]. 3rd ed. Beijing：China Agriculture Press，2000：108-268鲍士旦. 土壤农化分析[M]. 3版. 北京：中国农业出版社，2000：108-268
GUAN S Y. Soil enzymes and research methods[M]. Beijing：Agriculture Press，1986：274-329关松荫. 土壤酶及其研究法[M]. 北京：农业出版社，1986：274-329
LU X F，GUO J Y，WANG B，et al. Effects of nitrogen addition on plant above and below-ground biomass allocation in terrestrial ecosystems in China[J]. Acta Ecologica Sinica，2024，44（4）：1313-1323陆啸飞，郭洁芸，王斌，等. 氮添加对中国陆地植被地上-地下生物量分配的影响[J]. 生态学报，2024，44（4）：1313-1323
LE J J，SU Y，PENG Q W，et al. Effects of nitrogen addition on soil enzyme activities and ecoenzymatic stoichiometry in alpine grassland of the Tianshan Mountains[J]. Arid Zone Research，2020，37（2）：382-389勒佳佳，苏原，彭庆文，等. 氮添加对天山高寒草原土壤酶活性和酶化学计量特征的影响[J]. 干旱区研究，2020，37（2）：382-389
GUO W Q，XU X X，LU L，et al. Effects of warming and biochar addition on soil nutrients and microbial biomass in wheat fields[J]. Journal of Ecology and Rural Environment，2021，37（5）：611-618郭万庆，徐小逊，鲁兰，等. 增温和生物炭添加对麦田土壤养分和微生物生物量的影响[J]. 生态与农村环境学报，2021，37（5）：611-618
ZHAO N，WANG X L，HE J，et al. Effects of replacing chemical nitrogen fertilizer with organic fertilizer on active organic carbon fractions，enzyme activities，and crop yield in yellow soil[J]. Environmental Science，2024，45（7）：4196-4205赵娜，王小利，何进，等. 有机肥替代化学氮肥对黄壤活性有机碳组分、酶活性及作物产量的影响[J]. 环境科学，2024，45（7）：4196-4205
WANG L C，JIN Y B，SUN F X，et al. Effect of proportional fertilization on the growth of Sapindus mukoraiensis in Sichuan and Yunnan[J]. Agriculture and Technology，2024，44（3）：44-47王连春，金艳彬，孙风霞，等. 配比施肥对川滇无患子生长的影响[J]. 农业与技术，2024，44（3）：44-47
JING M H，JIA X T，ZHANG Y L，et al. Effects of long-term nitrogen addition on community aboveground and belowground biomass and their ratio in a typical steppe of Inner Mongolia[J]. Chinese Journal of Ecology，2020，39（10）：3185-3193景明慧，贾晓彤，张运龙，等. 长期氮添加对内蒙古典型草原植物地上、地下生物量及根冠比的影响[J]. 生态学杂志，2020，39（10）：3185-3193
YANG X T，FAN J，GE J M，et al. Soil physical and chemical properties and vegetation characteristics of different types of grassland in Qilian Mountains，China[J]. Chinese Journal of Applied Ecology，2022，33（4）：878-886杨学亭，樊军，盖佳敏，等. 祁连山不同类型草地的土壤理化性质与植被特征[J]. 应用生态学报，2022，33（4）：878-886
CHANG T，ZHANG Q，QIN R M，et al. Effects of nitrogen combined application on the vegetation and soil of degraded alpine meadow in the Three-River Headwaters Region[J]. Acta Agrestia Sinica，2023，31（3）：860-867常涛，张骞，秦瑞敏，等. 氮素配施对三江源区退化高寒草甸植被和土壤的影响[J]. 草地学报，2023，31（3）：860-867
JIANG S，ZHANG Z S，LI M，et al. Effect of short-term biochar addition on soil microbial community structure in degraded saline-alkali wetland[J]. Soils and Crops，2023，12（2）：225-233姜珊，张仲胜，李敏，等. 短期生物炭添加对退化盐碱湿地土壤微生物群落结构特征影响[J]. 土壤与作物，2023，12（2）：225-233
CUI Y F，SUN G C，GUO A N，et al. Effects of straw and biochar on soil physical properties and pH value in cold waterlogged paddy soils[J]. Jiangsu Agricultural Sciences，2020，48（21）：255-260崔月峰，孙国才，郭奥楠，等. 秸秆和生物炭还田对冷凉稻区土壤物理性质及pH值的影响[J]. 江苏农业科学，2020，48（21）：255-260
MATSCHONAT G，MATZNER E. Soil chemical properties affecting NH₄⁺ sorption in forest soils[J]. Zeitschrift Für Pflanzenernährung und Bodenkunde，1996，159（5）：505-511
YANG Y，LI C R，PENG Y，et al. Effects of biochar and organic fertilizer application on physical and chemical properties of red soil and nitrogen losses by runoff[J]. Soil and Fertilizer Sciences in China，2023（4）：16-24杨宇，李成蓉，彭银，等. 生物炭与有机肥施用对红壤理化性质及氮素径流损失的影响[J]. 中国土壤与肥料，2023（4）：16-24
WANG G F，LIU H Y，XING D， et al. Effect of pepper straw biochar added in seedling substrate on growth and nutrient of pepper seedlings[J]. Southwest China Journal of Agricultural Sciences，2022，35（3）：543-549王高飞，刘鸿雁，邢丹，等. 育苗基质添加辣椒秆生物炭对辣椒幼苗生长与养分的影响[J]. 西南农业学报，2022，35（3）：543-549
SUN C L，WANG Y W，WANG C J，et al. Effects of land use conversion on soil extracellular enzyme activity and its stoichiometric characteristics in karst mountainous areas[J]. Acta Ecologica Sinica，2021，41（10）：4140-4149孙彩丽，王艺伟，王从军，等. 喀斯特山区土地利用方式转变对土壤酶活性及其化学计量特征的影响[J]. 生态学报，2021，41（10）：4140-4149
LI X W，XIAO Y M，LI C L，et al. Effects of microtopography on enzyme activities and ecological stoichiometry in an alpine meadow on the Qinghai-Tibet Plateau[J]. Chinese Journal of Applied and Environmental Biology，2024，30（2）：212-219李欣蔚，肖元明，李春丽，等. 青藏高原高寒草甸微地形对土壤胞外酶活性及生态化学计量比的影响[J]. 应用与环境生物学报，2024，30（2）：212-219
MIAO Y，HOU R X，PEI H，et al. Impact of organic material incorporation on the chemical stoichiometry characteristics of extracellular enzymes in black soil[J]. Transactions of the Chinese Society of Agricultural Engineering，2023，39（22）：207-215苗宇，侯瑞星，裴浩，等. 有机物料还田对黑土胞外酶化学计量特征的影响[J]. 农业工程学报，2023，39（22）：207-215
DU L S，TANG M L，ZHU Z K，et al. Effects of long-term fertilization on enzyme activities in profile of paddy soil profiles[J]. Environmental Science，2018，39（8）：3901-3909杜林森，唐美铃，祝贞科，等. 长期施肥对不同深度稻田土壤碳氮水解酶活性的影响特征[J]. 环境科学，2018，39（8）：3901-3909
MENG F H，GAO J L，YU X F，et al. Improvment of biochemical property of surface soil by combined application of biochar with nitrogen fertilizer[J]. Journal of Plant Nutrition and Fertilizers，2018，24（5）：1214-1226孟繁昊，高聚林，于晓芳，等. 生物炭配施氮肥改善表层土壤生物化学性状研究[J]. 植物营养与肥料学报，2018，24（5）：1214-1226
PU C，KAN Z R，LIU P，et al. Residue management induced changes in soil organic carbon and total nitrogen under different tillage practices in the North China Plain[J]. Journal of Integrative Agriculture，2019，18（6）：1337-1347
JING Y P，LI Y J，YAO Y P，et al. Enzyme activities of saline-alkali soil and its relationship with soil microbial biomass and physicochemical factor[J]. Journal of Agricultural Science and Technology，2016，18（2）：128-138景宇鹏，李跃进，姚一萍，等. 盐渍化土壤酶活性及其与微生物、理化因子的关系[J]. 中国农业科技导报，2016，18（2）：128-138
YU Y， ZHANG C R， YANG Y L， et al. Effects of long-term no-tillage and different stover mulching amounts on soil carbon and nitrogen contents and enzyme activities of carbon and nitrogen cycle in Mollisols[J]. Chinese Journal of Applied Ecology，2024，35（3）：695-704于洋，张常仁，杨雅丽，等. 长期免耕和秸秆覆盖量对黑土碳氮含量及碳氮循环相关酶活性的影响[J]. 应用生态学报，2024，35（3）：695-704
SU J Q，LI X R，BAO J T. Effects of nitrogen addition on soil physico-chemical properties and enzyme activities in desertified steppe[J]. Chinese Journal of Applied Ecology，2014，25（3）：664-670苏洁琼，李新荣，鲍婧婷. 施氮对荒漠化草原土壤理化性质及酶活性的影响[J].应用生态学报，2014，25（3）：664-670
BAI M X，SITU G M，LI S H，et al. Effects of combined application of biochar with organic amendments on enzyme activity and microbial metabolic function of carbon sources in infertile red soil[J]. Chinese Journal of Applied Ecology，2022，33（5）：1283-1290白美霞，司徒高铭，李松昊，等. 生物质炭配施有机物料对贫瘠红壤酶活性和微生物碳源代谢功能的影响[J]. 应用生态学报，2022，33（5）：1283-1290
SHI D L，WANG X L，LIU A K，et al. Biological effect of biochar nitrogen replacing partial fertilizer nitrogen on rice in yellow soil[J]. Soil and Fertilizer Sciences in China，2021（2）：199-205史登林，王小利，刘安凯，等. 生物炭氮替代部分化肥氮对黄壤水稻的生物效应[J]. 中国土壤与肥料，2021（2）：199-205
ZHAO Y D， PAN Y Z. Impacts of reduced nitrogen combined with biochar application for many consecutive years on soil enzyme activity， microbial community and peanut yield[J]. Jiangsu Agricultural Sciences，2024，52（17）：260-267赵耀东，潘一展. 连续多年减氮配施生物炭对土壤酶活性、微生物群落及花生产量的影响[J]. 江苏农业科学，2024，52（17）：260-267)