Article(id=1309569822111925058, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.03.005, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1750867200000, receivedDateStr=2025-06-26, revisedDate=1754928000000, revisedDateStr=2025-08-12, acceptedDate=null, acceptedDateStr=null, onlineDate=1790155376221, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790155376221, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790155376221, creator=13701087609, updateTime=1790155376221, updator=13701087609, issue=Issue{id=1309569821424051089, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='3', pageStart='745', pageEnd='1136', issueExtLink='null', onlineDate='null', pubDate='1773504000000', pubDateStr='2026-03-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790155376058, creator='13701087609', updateTime=1790156773348, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575682133422151, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575682133422152, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=788, endPage=795, ext={EN=ArticleExt(id=1309569822527161156, articleId=1309569822111925058, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Chromosomal Ploidy and Karyotype Analysis of 5 Plant Species of Gynostemma Genus, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=This study employed conventional chromosome squash techniques to analyze the ploidy levels and karyotypes of eight samples from five Gynostemma species, aiming to investigate their evolutionary trends and phylogenetic relationships. The results revealed that all eight samples exhibited a basic chromosome number of 11. Among them, G.pentaphyllum collected from Fujian Nanjing was tetraploid, while the remaining seven samples were diploid. Satellite chromosomes were observed in two samples. Chromosome types included two categories: m (metacentric) and sm (submetacentric). Karyotype classifications comprised four types: 1A, 2A, 1B, and 2B. The ratio of the longest to shortest chromosome ranged from 1.48 to 3.26, with an average arm ratio of 1.26-1.45. The karyotypic asymmetry coefficient varied between 55.59% and 58.56%. Based on karyotype evolution, the samples were ordered as follows: GXH, WZ>GXY>AH, FJ>MD, GXB, YJ. These findings provide cytological evidence for the taxonomic classification and phylogenetic analysis of Gynostemma species., authors=CHEN Shuang1 , CAO Feng1 , LI Dou-dou1 , GUO Bao-lin1 , SUO Feng-mei1 , CAO Xian-jun2 , LI Xiao-hu2 , authorsList=CHEN Shuang, CAO Feng, LI Dou-dou, GUO Bao-lin, SUO Feng-mei, CAO Xian-jun, LI Xiao-hu, authorCompany=1 Institute of Medicinal Plants Development, Chinese Academy of Medical Sciences, Beijing 100193; 2 Shaanxi Cuiyuankang Agricultural Technology, Ankang, Shaanxi Province 725000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569822409720643, articleId=1309569822111925058, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=绞股蓝属5种植物的染色体倍性及核型分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=本研究利用常规染色体压片技术,对绞股蓝属5个物种8个样品的倍性及核型进行分析,探讨它们之间的进化趋势和亲缘关系,发现8个样品的染色体基数均为11,除福建南靖产绞股蓝Gynostemma pentaphyllum 为四倍体,其余均为二倍体;8个样品中2个样品有随体,染色体类型包括m,sm 2种,核型有1A,2A,1B,2B 4个类型,最长染色体/最短染色体值1.48~3.26,平均臂比值1.26~1.45,核不对称系数55.59%~58.56%。按照核型类型的进化程度分别为GXH,WZ>GXY>AH,FJ>MD,GXB、YJ。这些数据为绞股蓝属植物分类和系统关系研究提供了细胞学依据。, authors=陈霜1 , 曹峰1 , 李豆豆1 , 郭宝林1 , 索凤梅1 , 曹显俊2 , 李晓虎2 , authorsList=陈霜, 曹峰, 李豆豆, 郭宝林, 索凤梅, 曹显俊, 李晓虎, authorCompany=1 中国医学科学院药用植物研究所, 北京 100193; 2 陕西萃源康农业科技有限公司, 陕西 安康 725000, correspAuthors=null, authorNote=陈霜(2004-),女,汉族,四川南充人,硕士研究生,主要从事绞股蓝种质资源研究,E-mail:cs17738210796@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, 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BMC Plant Biology ,2024,24(1):205 QIN S S,LI H T, WANG Z Y,et al. Analysis phylogenetic relationship of Gynostemma (Cucurbitaceae)[J]. China Journal of Chinese Materia Medica ,2015,40(9):1681-1687秦双双,李海涛,汪周勇,等. 绞股蓝属植物亲缘关系初步分析[J]. 中国中药杂志,2015,40(9):1681-1687 SUN H,CHEN S K. The microstructural features of seed surfaces and its taxonomic significance in the genus Gynostemma [J]. Plant Diversity ,1998,20(3):309-311孙航,陈书坤. 绞股蓝属植物种皮微结构特征及其分类学意义[J]. 云南植物研究,1998,20(3):309-311 LI R J,SHANG Z Y,ZHANG J Z. The chromosome observation on 3 species in the genus Gynostemma [J]. Chinese Bulletin of Botany ,1989,24(4):245-247李汝娟,尚宗燕,张继祖. 三种绞股蓝植物染色体观察[J]. 植物学通报,1989,24(4):245-247 GAO X F,CHEN S K,GU Z J,et al. A chromosomal study on the genus Gynostemma (Cucurbitaceae)[J]. Plant Diversity ,1995,17(3):312-316高信芬,陈书坤,顾志建,等. 绞股蓝属的染色体研究[J]. 云南植物研究,1995,17(3):312-316 YANG Q Y,WEI P H,LI G L. A study on the karyotype of Gynostemma laxiflorum [J]. Journal of Nanjing Normal University (Natural Science Edition) ,1995,18(1):85-87,93杨启银,韦平和,李桂兰. 疏花绞股蓝的核型研究[J]. 南京师大学报(自然科学版),1995,18(1):85-87,93 HUANG X Y,NONG D X,HUANG B Y,et al. Gynostemma verrucosum (Cucurbitaceae),a new species with verrucose stem from Guangxi,China[J]. Phytotaxa ,2024,649(3):293-300 CHEN S K,LU A M,CHARLES J. Flora of China [M]. Beijing:Science Press,2011:11-15 QU R J,LIU Y P,CHEN J Y,et al. Chromosome karyotype analysis from six different populations of Sphaerophysa salsula (Fabaceae)[J]. Acta Agrestia Sinica ,2024,32(8):2469-2477曲荣举,刘玉萍,陈金元,等. 苦马豆(Sphaerophysa salsula )6个不同居群的染色体核型分析[J]. 草地学报,2024,32(8):2469-2477 LI M X, CHEN R Y. A suggestion on the standardization of karyotype analysis in plants[J]. Plant Science Journal ,1985,3(4):297-302李懋学,陈瑞阳. 关于植物核型分析的标准化问题[J]. 武汉植物学研究,1985,3(4):297-302 LEVAN A,FREDGA K,SANDBERG A A. Nomenclature for centromeric position on chromosomes[J]. Hereditas ,1964,52(2):201-220 ARANO H. Cytological studies in subfamily carduoideae (Compositae) of Japan XII[J]. Shokubutsugaku Zasshi ,1963,76(900):219-224 STEBBINS G L. Chromosomal evolution in higher plants [M]. London:Edward Arnold,1971:20-30 KUO S K, WANG T T, HUANG T C. Karyotype analysis of some Formosan gymnosperms[J]. Taiwania , 1972, 17(1): 66-80 LI M X,ZHANG F. Plant Chromosome research technology [M]. Harbin:Northeast Forestry University Press, 1991:1-30李懋学,张方. 植物染色体研究技术[M]. 哈尔滨:东北林业大学出版社,1991:1-30 TAO D H,LI X H,WANG L Q,et al. Research progress on identification methods of plant chromosome ploidy[J]. Life Science Research ,2009,13(5):453-458陶抵辉,李小红,王利群,等. 植物染色体倍性鉴定方法研究进展[J]. 生命科学研究,2009,13(5):453-458 JIN J R,LIU Y P,SU X,et al. Chromosome karyotype studies on different populations of Xanthopappus Subacaulis (Asteraceae),an endemic species from the Qinghai-Xizang Plateau[J]. Acta Agrestia Sinica ,2025,33(1):53-61靳佳瑞,刘玉萍,苏旭,等. 青藏高原特有种—黄缨菊不同居群的染色体核型研究[J]. 草地学报,2025,33(1):53-61 WANG Z X,WEI C,LIAN Y Q,et al. Karyotype analysis of four Lilium species from China[J]. Acta Horticulturae Sinica ,2013,40(11):2207-2212王中轩,魏迟,廉玉芹,等. 中国原产4种野生百合的核型分析[J]. 园艺学报,2013,40(11):2207-2212 SUN H N,LI M Y,LIU D Y,et al. Chromosome number identification and karyotype analysis of Coreopsis L[J]. Acta Agrestia Sinica ,2021,29(11):2477-2485孙浩男,李明阳,刘冬云,等. 不同种(品种)金鸡菊的染色体数目鉴定及核型分析[J]. 草地学报,2021,29(11):2477-2485 ALAM M,HAQU E, GHOSH B. Karyomorphological studies of six commercially cultivated edible cucurbits: bitter gourd, sponge gourd, ridge gourd, snake gourd, ash gourd and cucumber[J]. Caryologia ,2018,71(2):150-159 LI M X,YAN X C. Studies on karyotypes of some wild and cultivated tea in China[J]. Journal of Wuhan Botanical Research ,1985,3(4):319-324,491-493李懋学,严学成. 中国某些野生和栽培茶的核型研究[J]. 武汉植物学研究,1985,3(4):319-324,491-493)
草地学报
|研究论文
2026
, 34
(3) :
788
-795
绞股蓝属5种植物的染色体倍性及核型分析
全屏
陈霜1 , 曹峰1 , 李豆豆1 , 郭宝林1 , 索凤梅1 , 曹显俊2 , 李晓虎2
作者信息
1 中国医学科学院药用植物研究所, 北京 100193; 2 陕西萃源康农业科技有限公司, 陕西 安康 725000
作者简介:
陈霜(2004-),女,汉族,四川南充人,硕士研究生,主要从事绞股蓝种质资源研究,E-mail:cs17738210796@163.com
Chromosomal Ploidy and Karyotype Analysis of 5 Plant Species of Gynostemma Genus
CHEN Shuang1 , CAO Feng1 , LI Dou-dou1 , GUO Bao-lin1 , SUO Feng-mei1 , CAO Xian-jun2 , LI Xiao-hu2
Affiliations
1 Institute of Medicinal Plants Development, Chinese Academy of Medical Sciences, Beijing 100193; 2 Shaanxi Cuiyuankang Agricultural Technology, Ankang, Shaanxi Province 725000, China
doi: 10.11733/j.issn.1007-0435.2026.03.005
文章导航
本研究利用常规染色体压片技术,对绞股蓝属5个物种8个样品的倍性及核型进行分析,探讨它们之间的进化趋势和亲缘关系,发现8个样品的染色体基数均为11,除福建南靖产绞股蓝Gynostemma pentaphyllum 为四倍体,其余均为二倍体;8个样品中2个样品有随体,染色体类型包括m,sm 2种,核型有1A,2A,1B,2B 4个类型,最长染色体/最短染色体值1.48~3.26,平均臂比值1.26~1.45,核不对称系数55.59%~58.56%。按照核型类型的进化程度分别为GXH,WZ>GXY>AH,FJ>MD,GXB、YJ。这些数据为绞股蓝属植物分类和系统关系研究提供了细胞学依据。
This study employed conventional chromosome squash techniques to analyze the ploidy levels and karyotypes of eight samples from five Gynostemma species, aiming to investigate their evolutionary trends and phylogenetic relationships. The results revealed that all eight samples exhibited a basic chromosome number of 11. Among them, G.pentaphyllum collected from Fujian Nanjing was tetraploid, while the remaining seven samples were diploid. Satellite chromosomes were observed in two samples. Chromosome types included two categories: m (metacentric) and sm (submetacentric). Karyotype classifications comprised four types: 1A, 2A, 1B, and 2B. The ratio of the longest to shortest chromosome ranged from 1.48 to 3.26, with an average arm ratio of 1.26-1.45. The karyotypic asymmetry coefficient varied between 55.59% and 58.56%. Based on karyotype evolution, the samples were ordered as follows: GXH, WZ>GXY>AH, FJ>MD, GXB, YJ. These findings provide cytological evidence for the taxonomic classification and phylogenetic analysis of Gynostemma species.
Gynostemma
/
Chromosomes
/
Ploidy
/
Karyotype
陈霜, 曹峰, 李豆豆, 郭宝林, 索凤梅, 曹显俊, 李晓虎.
绞股蓝属5种植物的染色体倍性及核型分析.
草地学报,
2026
, 34
(3)
: 788
-795
.
DOI: 10.11733/j.issn.1007-0435.2026.03.005
CHEN Shuang, CAO Feng, LI Dou-dou, GUO Bao-lin, SUO Feng-mei, CAO Xian-jun, LI Xiao-hu.
Chromosomal Ploidy and Karyotype Analysis of 5 Plant Species of Gynostemma Genus[J].
Acta Agrestia Sinica ,
2026
, 34
(3)
: 788
-795
.
DOI: 10.11733/j.issn.1007-0435.2026.03.005
参考文献
引证文献
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Acta Botanica Boreali-Occidentalia Sinica ,2012,32(10):2133-2138李忠虎,刘占林,赵鹏,等. 绞股蓝属植物系统进化研究进展述评[J]. 西北植物学报,2012,32(10):2133-2138 YUN L L,ZHANG C Y,LIANG T T,et al. Insights into dammarane-type triterpenoid saponin biosynthesis from the telomere-to-telomere genome of Gynostemma pentaphyllum [J]. Plant Communications ,2024,5(8):100932 ZHANG X,YANG H,WANG X,et al. Genome-wide identification and characterization of OSC gene family in Gynostemma pentaphyllum (Cucurbitaceae)[J]. Life ,2024,14(12):1599 HUANG D,MING R H,XU S Q,et al. Genome-wide identification of R2R3-MYB transcription factors: discovery of a “dual-function” regulator of gypenoside and flavonol biosynthesis in Gynostemma pentaphyllum [J]. Frontiers in Plant Science ,2021,12:796248 QIN Y H,LI J M,CHEN J H,et al. Genome-wide characterization of the bHLH gene family in Gynostemma pentaphyllum reveals its potential role in the regulation of gypenoside biosynthesis[J]. BMC Plant Biology ,2024,24(1):205 QIN S S,LI H T, WANG Z Y,et al. Analysis phylogenetic relationship of Gynostemma (Cucurbitaceae)[J]. China Journal of Chinese Materia Medica ,2015,40(9):1681-1687秦双双,李海涛,汪周勇,等. 绞股蓝属植物亲缘关系初步分析[J]. 中国中药杂志,2015,40(9):1681-1687 SUN H,CHEN S K. The microstructural features of seed surfaces and its taxonomic significance in the genus Gynostemma [J]. Plant Diversity ,1998,20(3):309-311孙航,陈书坤. 绞股蓝属植物种皮微结构特征及其分类学意义[J]. 云南植物研究,1998,20(3):309-311 LI R J,SHANG Z Y,ZHANG J Z. The chromosome observation on 3 species in the genus Gynostemma [J]. Chinese Bulletin of Botany ,1989,24(4):245-247李汝娟,尚宗燕,张继祖. 三种绞股蓝植物染色体观察[J]. 植物学通报,1989,24(4):245-247 GAO X F,CHEN S K,GU Z J,et al. A chromosomal study on the genus Gynostemma (Cucurbitaceae)[J]. Plant Diversity ,1995,17(3):312-316高信芬,陈书坤,顾志建,等. 绞股蓝属的染色体研究[J]. 云南植物研究,1995,17(3):312-316 YANG Q Y,WEI P H,LI G L. A study on the karyotype of Gynostemma laxiflorum [J]. Journal of Nanjing Normal University (Natural Science Edition) ,1995,18(1):85-87,93杨启银,韦平和,李桂兰. 疏花绞股蓝的核型研究[J]. 南京师大学报(自然科学版),1995,18(1):85-87,93 HUANG X Y,NONG D X,HUANG B Y,et al. Gynostemma verrucosum (Cucurbitaceae),a new species with verrucose stem from Guangxi,China[J]. Phytotaxa ,2024,649(3):293-300 CHEN S K,LU A M,CHARLES J. Flora of China [M]. Beijing:Science Press,2011:11-15 QU R J,LIU Y P,CHEN J Y,et al. Chromosome karyotype analysis from six different populations of Sphaerophysa salsula (Fabaceae)[J]. Acta Agrestia Sinica ,2024,32(8):2469-2477曲荣举,刘玉萍,陈金元,等. 苦马豆(Sphaerophysa salsula )6个不同居群的染色体核型分析[J]. 草地学报,2024,32(8):2469-2477 LI M X, CHEN R Y. A suggestion on the standardization of karyotype analysis in plants[J]. Plant Science Journal ,1985,3(4):297-302李懋学,陈瑞阳. 关于植物核型分析的标准化问题[J]. 武汉植物学研究,1985,3(4):297-302 LEVAN A,FREDGA K,SANDBERG A A. Nomenclature for centromeric position on chromosomes[J]. Hereditas ,1964,52(2):201-220 ARANO H. Cytological studies in subfamily carduoideae (Compositae) of Japan XII[J]. Shokubutsugaku Zasshi ,1963,76(900):219-224 STEBBINS G L. Chromosomal evolution in higher plants [M]. London:Edward Arnold,1971:20-30 KUO S K, WANG T T, HUANG T C. Karyotype analysis of some Formosan gymnosperms[J]. Taiwania , 1972, 17(1): 66-80 LI M X,ZHANG F. Plant Chromosome research technology [M]. Harbin:Northeast Forestry University Press, 1991:1-30李懋学,张方. 植物染色体研究技术[M]. 哈尔滨:东北林业大学出版社,1991:1-30 TAO D H,LI X H,WANG L Q,et al. Research progress on identification methods of plant chromosome ploidy[J]. Life Science Research ,2009,13(5):453-458陶抵辉,李小红,王利群,等. 植物染色体倍性鉴定方法研究进展[J]. 生命科学研究,2009,13(5):453-458 JIN J R,LIU Y P,SU X,et al. Chromosome karyotype studies on different populations of Xanthopappus Subacaulis (Asteraceae),an endemic species from the Qinghai-Xizang Plateau[J]. Acta Agrestia Sinica ,2025,33(1):53-61靳佳瑞,刘玉萍,苏旭,等. 青藏高原特有种—黄缨菊不同居群的染色体核型研究[J]. 草地学报,2025,33(1):53-61 WANG Z X,WEI C,LIAN Y Q,et al. Karyotype analysis of four Lilium species from China[J]. Acta Horticulturae Sinica ,2013,40(11):2207-2212王中轩,魏迟,廉玉芹,等. 中国原产4种野生百合的核型分析[J]. 园艺学报,2013,40(11):2207-2212 SUN H N,LI M Y,LIU D Y,et al. Chromosome number identification and karyotype analysis of Coreopsis L[J]. Acta Agrestia Sinica ,2021,29(11):2477-2485孙浩男,李明阳,刘冬云,等. 不同种(品种)金鸡菊的染色体数目鉴定及核型分析[J]. 草地学报,2021,29(11):2477-2485 ALAM M,HAQU E, GHOSH B. Karyomorphological studies of six commercially cultivated edible cucurbits: bitter gourd, sponge gourd, ridge gourd, snake gourd, ash gourd and cucumber[J]. Caryologia ,2018,71(2):150-159 LI M X,YAN X C. Studies on karyotypes of some wild and cultivated tea in China[J]. Journal of Wuhan Botanical Research ,1985,3(4):319-324,491-493李懋学,严学成. 中国某些野生和栽培茶的核型研究[J]. 武汉植物学研究,1985,3(4):319-324,491-493
2026年第34卷第3期
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doi: 10.11733/j.issn.1007-0435.2026.03.005
接收时间:2025-06-26
首发时间:2026-09-23
收稿日期:2025-06-26
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2种不同金属材料的力学参数
科 Family 属数 Number of genus 种数 Number of species 占总种数比例 Percentage of total species (%) 属 Genus 种数 Number of species 占总种数比例 Percentage of total species (%) 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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