Article(id=1309569826440442623, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.03.003, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1745251200000, receivedDateStr=2025-04-22, revisedDate=1749312000000, revisedDateStr=2025-06-08, acceptedDate=null, acceptedDateStr=null, onlineDate=1790155377253, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790155377253, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790155377253, creator=13701087609, updateTime=1790155377253, updator=13701087609, issue=Issue{id=1309569821424051089, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='3', pageStart='745', pageEnd='1136', issueExtLink='null', onlineDate='null', pubDate='1773504000000', pubDateStr='2026-03-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790155376058, creator='13701087609', updateTime=1790156773348, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575682133422151, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575682133422152, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=770, endPage=778, ext={EN=ArticleExt(id=1309569826843095809, articleId=1309569826440442623, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Nano-Iron Priming on Seed Germination and Physiological Characteristics of Seedling of Agropyron mongolicum under Salt Stress, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=The development and utilization of saline-alkali land is a key focus for grassland improvement in China, and enhancing the germination capacity of native grass seeds is a crucial step of this process. In this study seeds of Mongolian wheatgrass (Agropyron mongolicum) was used as the material to investigate the effects of nano-iron particle suspensions at different concentrations (25, 50, 75 and 100 mg·L-1) and different priming duration (4 h and 36 h) on seeds germination, seedling growth characteristics and antioxidant enzyme activities of seedling under 175 mmol·L-1 mixed salt solution stress (NaCl∶Na2SO4=5∶1). Multispectral imaging technology was also used to rapidly and visually assess seedling vigor and to screen for the most salt-tolerant priming treatments. The results showed that the priming treatment with 4 h significantly outperformed than the 36 h treatment according to the analysis of seed germination and seedling growth indexes (P<0.05). Nano-iron priming for 4 h at all concentrations significantly improved the germination percentage and germination index (P<0.05), reduced mean germination time, and increased seedling height, fresh weight, vigor index, and antioxidant enzyme activities (e.g. POD, CAT, APX and MDHAR) in the seedlings (P<0.05). The nCDA images from multispectral imaging were highly consistent with the trends of these physiological and biochemical changes. Among all the treatments, 50 mg·L-1 nano-iron priming for 4 h was the most effective, significantly enhancing germinability and growth performance of Mongolian wheatgrass under salt stress and markedly improving its salt tolerance. These findings provide important scientific evidence for vegetation restoration and ecological rehabilitation in saline-alkali lands., authors=HU Xin-yu1, LI Chang-ran1, LI Ruo-hong1, FU Jia-yi1, MAO Pei-sheng1, authorsList=HU Xin-yu, LI Chang-ran, LI Ruo-hong, FU Jia-yi, MAO Pei-sheng, authorCompany=1 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569826729849600, articleId=1309569826440442623, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=纳米铁引发对盐胁迫下蒙古冰草种子发芽及幼苗生理特性的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=盐碱地的开发利用是我国草地改良的重点,提高乡土草种种子发芽能力是草地建设的关键环节。试验以蒙古冰草(Agropyron mongolicum)种子为材料,在175 mmol·L⁻¹混合盐溶液胁迫下(NaCl∶Na₂SO₄=5∶1),探究不同浓度(25,50,75和100 mg·L⁻¹)纳米铁颗粒悬浮液和不同时间(4 h和36 h)的引发处理对于蒙古冰草种子发芽、幼苗生长特性和抗氧化酶活性的影响,并利用多光谱成像技术快速直观地判定幼苗活力,筛选耐盐性强的引发处理。结果表明:在种子发芽和幼苗生长相关指标测定中,引发4 h效果显著优于36 h(P<0.05)。4 h引发处理时不同浓度的纳米铁能显著提高蒙古冰草的发芽率和发芽指数(P<0.05),平均发芽时间缩短,幼苗的苗长、鲜重、活力指数及幼苗中过氧化物酶(Peroxidase,POD)、过氧化氢酶(Catalase,CAT)、抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)和单脱氢抗坏血酸还原酶(Monodehydroascorbate reductase,MDHAR)等抗氧化酶活性显著提高(P<0.05),多光谱成像获取的归一化典型判别分析(nCDA)图像结果与上述生理生化指标变化趋势高度一致。综合分析,50 mg·L⁻¹纳米铁引发蒙古冰草种子4 h处理效果最佳,能有效增强蒙古冰草在盐胁迫下的发芽与生长性能,显著提升其耐盐性,为盐碱地植被恢复和生态修复提供了重要的科学依据。, authors=胡新雨1, 李长然1, 李若鸿1, 傅佳怡1, 毛培胜1, authorsList=胡新雨, 李长然, 李若鸿, 傅佳怡, 毛培胜, authorCompany=1 中国农业大学草业科学与技术学院, 北京 100193, correspAuthors=null, authorNote=胡新雨(2002-),女,汉族,江西南昌人,硕士研究生,主要从事草类种子生理与生产研究,E-mail:huxy@cau.edu.cn, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=LGCqEWHcmbaT03Im+lZk0A==, pdfFileSize=1720003, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=内蒙古自治区种业科技创新重大示范工程“揭榜挂帅”项目 (2022JBGS0014))}, authors=null, keywords=[Keyword(id=1309569826998285058, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569826440442623, language=CN, orderNo=1, keyword=蒙古冰草), Keyword(id=1309569827086365443, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569826440442623, language=CN, orderNo=2, keyword=耐盐性), Keyword(id=1309569827182834436, tenantId=1146029695717560320, journalId=1308397196074385509, 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Effects of enclosure on biomass allocation characteristics of Agropyron mongolicum population in desert steppe[J]. Ecology and Environmental Sciences,2017,26(12):2024-2029赵盼盼,邵文山,靳长青,等. 围封对荒漠草原沙芦草种群构件生物量分配特性的影响[J]. 生态环境学报,2017,26(12):2024-2029 LI X Q,GAO Y H,LIU Y,et al. The genetic diversity of 9 populations of dry-desert Agropyron mongolicumcollected in northern China[J]. Acta Prataculturae Sinica,2016,25(3):77-85李晓全,高有汉,刘扬,等. 我国北方9份旱生—沙生植物蒙古冰草遗传多样性研究[J]. 草业学报,2016,25(3):77-85 ZHOU L L,FU B Z,XU D M,et al. Effects of salt stress on germination characteristics of Agropyron mongolicum and salt-tolerance evaluation[J]. Pratacultural Science,2015,32(8):1252-1259周璐璐,伏兵哲,许冬梅,等. 盐胁迫对沙芦草萌发特性影响及耐盐性评价[J]. 草业科学,2015,32(8):1252-1259 LIU X C,HU H J,TIAN Y,et al. Effects of exogenous silicon nanosilicon on seed germination and physiological indicators of Capsicum annuum seeds under salt stress[J]. Seed,2025,44(4):26-33刘小岑,胡海骏,田野,等. 外源纳米硅对盐胁迫下辣椒种子萌发及生理指标的影响[J]. 种子,2025,44(4):26-33 FRACETO L F,GRILLO R,DE MEDEIROS G A,et al. Nanotechnology in agriculture: which innovation potential does it have?[J]. Frontiers in Environmental Science,2016,4:20 SRIVASTAVA P,BRAGANÇA J,RAMANAN S R,et al. Synthesis of silver nanoparticles using haloarchaeal isolate Halococcus salifodinae BK3[J]. Extremophiles,2013,17(5):821-831 ANAND A,KUMARI A,THAKUR M,et al. Hydrogen peroxide signaling integrates with phytohormones during the germination of magnetoprimed tomato seeds[J]. Scientific Reports,2019,9:8814 CHUNG I M,REKHA K,RAJAKUMAR G,et al. Influence of silver nanoparticles on the enhancement and transcriptional changes of glucosinolates and phenolic compounds in genetically transformed root cultures of Brassica rapa ssp. Rapa[J]. Bioprocess and Biosystems Engineering,2018,41(11):1665-1677 YOU P,HE X Q. Recent progress in seed nanopriming research[J]. Pratacultural Science,2020,37(8):1548-1557尤沛,何学青. 种子纳米引发的研究进展[J]. 草业科学,2020,37(8):1548-1557 ELKHATIB E,ATTIA M,MAHDY A,et al. Priming with mango peels nanoparticles enhances seed germination of maize (Zea mays L.) under salt stress[J]. Alexandria Science Exchange Journal,2019,40(4):767-780 AFZAL S,SHARMA D,SINGH N K. Eco-friendly synthesis of phytochemical-capped iron oxide nanoparticles as nano-priming agent for boosting seed germination in rice (Oryza sativa L.)[J]. Environmental Science and Pollution Research,2021,28(30):40275-40287 ABOU-ZEID H M,ISMAIL G S M,ABDEL-LATIF S A. Influence of seed priming with ZnO nanoparticles on the salt-induced damages in wheat (Triticum aestivum L.) plants[J]. Journal of Plant Nutrition,2021,44(5):629-643 YUE J M,CONG X X,LI M L. Application of multispectral imaging technology in seed quality detection[J]. Seed,2021,40(10):129-135岳佳铭,丛晓翔,李曼莉. 多光谱成像技术在种子质量检测研究中的应用[J]. 种子,2021,40(10):129-135 ELMASRY G,MANDOUR N,WAGNER M H,et al. Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds[J]. Plant Methods,2019,15:24 ZHANG H,YAO X G,ZHANG X B,et al. Measurement of rice leaf chlorophyll and seed nitrogen contents by using multi-spectral imagine[J]. Chinese Journal of Rice Science,2008,22(5):555-558张浩,姚旭国,张小斌,等. 基于多光谱图像的水稻叶片叶绿素和籽粒氮素含量检测研究[J]. 中国水稻科学,2008,22(5):555-558 REN L,LIU W,LIU C H,et al. Non-destructive detection of Aspergillus ochraceus in corn based on multispectral imaging technology[J]. Journal of Chinese Institute of Food Science and Technology,2024,24(6):402-409任林,刘伟,刘长虹,等. 基于多光谱成像技术的玉米赭曲霉菌无损检测[J]. 中国食品学报,2024,24(6):402-409 WU H Z. Research on grape sweetness detection technology based on multi-spectral imaging technology[D]. Changchun:Changchun University of Science and Technology,2024:36-42吴虹璋. 基于多光谱成像技术葡萄甜度检测技术研究[D]. 长春:长春理工大学,2024:36-42 General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China. Grass seed testing procedures—Germination test: GB/T 2930.4—2017[S]. Beijing:Standards Press of China,2017:1-22国家质量监督检验检疫总局中国国家标准化管理委员会. 草种子检验规程发芽试验:GB/T 2930.4—2017[S]. 北京:中国标准出版社,2017:1-22 DUAN B B,WEI Z D,WANG L. Analyzing the impacts of cadmium alone and in co-existence with polypropylene microplastics on seed germination and seedling growth of maize[J]. Plastics Science and Technology,2024,52(8):83-88段冰冰,魏张东,王琳. 重金属镉单独或与聚丙烯微塑料共同暴露对玉米种子萌发及幼苗生长的影响研究[J]. 塑料科技,2024,52(8):83-88 CAKMAK I,MARSCHNER H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves[J]. Plant Physiology,1992,98(4):1222-1227 KAR M,MISHRA D. Catalase,peroxidase,and polyphenoloxidase activities during rice leaf senescence[J]. Plant Physiology,1976,57(2):315-319 HOSSAIN Z,LÓPEZ-CLIMENT M F,ARBONA V,et al. Modulation of the antioxidant system in Citrus under waterlogging and subsequent drainage[J]. Journal of Plant Physiology,2009,166(13):1391-1404 ARRIGONI O,DIPIERRO S,BORRACCINO G. Ascorbate free radical reductase,a key enzyme of the ascorbic acid system[J]. FEBS Letters,1981,125(2):242-244 NAKANO Y,ASADA K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J]. Plant and Cell Physiology,1981,22(5):867-880 SHEN Z B,PAN D F,WANG J L,et al. Effects of saline-alkaloid stress on seed germination and seedling growth of grasses[J]. Acta Agrestia Sinica,2012,20(5):914-920申忠宝,潘多锋,王建丽,等. 混合盐碱胁迫对5种禾草种子萌发及幼苗生长的影响[J]. 草地学报,2012,20(5):914-920 WANG R H,HU H Z,LIU L,et al. Effects of exogenous spermidine on seed germination of sugar beet under salt stress[J]. Journal of Anhui Agricultural Sciences,2025,53(8):129-132王仁汉,胡海洲,刘蕾,等. 亚精胺对盐胁迫下甜菜种子萌发的影响[J]. 安徽农业科学,2025,53(8):129-132 SUN Y F. Effects of nanopriming with iron oxide (Ⅲ) on seed germination and drought resistance of switchgrass (Panicum virgatum)[D]. Yangling:Northwest A & F University,2021:22-26孙运府. 纳米铁引发对柳枝稷种子萌发特性及抗旱性的影响[D]. 杨凌:西北农林科技大学,2021:22-26 ZHAO D Q,FANG Z W,TANG Y H,et al. Graphene oxide as an effective soil water retention agent can confer drought stress tolerance to Paeonia ostii without toxicity[J]. Environmental Science & Technology,2020,54(13):8269-8279 SONG H,GENG Q L,WU X,et al. The transcription factor MYC1 interacts with FIT to negatively regulate iron homeostasis in Arabidopsis thaliana[J]. The Plant Journal,2023,114(1):193-208 LEE C W,MAHENDRA S,ZODROW K,et al. Developmental phytotoxicity of metal oxide nanoparticles to Arabidopsis thaliana[J]. Environmental Toxicology and Chemistry,2010,29(3):669-675 DURAN N M,SAVASSA S M,DE LIMA R G,et al. X-ray spectroscopy uncovering the effects of Cu based nanoparticle concentration and structure on Phaseolus vulgaris germination and seedling development[J]. Journal of Agricultural and Food Chemistry,2017,65(36):7874-7884 SZÖLLÖSI R,MOLNÁR Á,KONDAK S,et al. Dual effect of nanomaterials on germination and seedling growth: stimulation vs. phytotoxicity[J]. Plants,2020,9(12):1745 YU Y. Research on nano-priming and film coating technology of oat seeds resistant to UV-B stress[D]. Beijing:China Agricultural University,2024:18-19于洋. 基于纳米引发的耐UV-B型燕麦种子包膜技术研究[D].北京:中国农业大学,2024:18-19 DAS K,ROYCHOUDHURY A. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants[J]. Frontiers in Environmental Science,2014,2:53 NADARAJAH K K. ROS homeostasis in abiotic stress tolerance in plants[J]. International Journal of Molecular Sciences,2020,21(15):5208 MAZHAR M W,ISHTIAQ M,MAQBOOL M,et al. Seed priming with Calcium oxide nanoparticles improves germination,biomass,antioxidant defence and yield traits of canola plants under drought stress[J]. South African Journal of Botany,2022,151:889-899 DAM P,PARET M L,MONDAL R,et al. Advancement of noble metallic nanoparticles in agriculture: A promising future[J]. Pedosphere,2023,33(1):116-128 SHETTY N,OLESEN M H,GISLUM R,et al. Use of partial least squares discriminant analysis on visible-near infrared multispectral image data to examine germination ability and germ length in spinach seeds[J]. Journal of Chemometrics,2012,26(8/9):462-466)
The development and utilization of saline-alkali land is a key focus for grassland improvement in China, and enhancing the germination capacity of native grass seeds is a crucial step of this process. In this study seeds of Mongolian wheatgrass (Agropyron mongolicum) was used as the material to investigate the effects of nano-iron particle suspensions at different concentrations (25, 50, 75 and 100 mg·L-1) and different priming duration (4 h and 36 h) on seeds germination, seedling growth characteristics and antioxidant enzyme activities of seedling under 175 mmol·L-1 mixed salt solution stress (NaCl∶Na2SO4=5∶1). Multispectral imaging technology was also used to rapidly and visually assess seedling vigor and to screen for the most salt-tolerant priming treatments. The results showed that the priming treatment with 4 h significantly outperformed than the 36 h treatment according to the analysis of seed germination and seedling growth indexes (P<0.05). Nano-iron priming for 4 h at all concentrations significantly improved the germination percentage and germination index (P<0.05), reduced mean germination time, and increased seedling height, fresh weight, vigor index, and antioxidant enzyme activities (e.g. POD, CAT, APX and MDHAR) in the seedlings (P<0.05). The nCDA images from multispectral imaging were highly consistent with the trends of these physiological and biochemical changes. Among all the treatments, 50 mg·L-1 nano-iron priming for 4 h was the most effective, significantly enhancing germinability and growth performance of Mongolian wheatgrass under salt stress and markedly improving its salt tolerance. These findings provide important scientific evidence for vegetation restoration and ecological rehabilitation in saline-alkali lands.
Key words
Mongolian wheatgrass
/
Salt tolerance
/
Seed priming
/
Nano-iron
HU Xin-yu, LI Chang-ran, LI Ruo-hong, FU Jia-yi, MAO Pei-sheng.
Effects of Nano-Iron Priming on Seed Germination and Physiological Characteristics of Seedling of Agropyron mongolicum under Salt Stress[J].
Acta Agrestia Sinica,
2026
, 34
(3)
: 770
-778
.
DOI: 10.11733/j.issn.1007-0435.2026.03.003
基金
收起
内蒙古自治区种业科技创新重大示范工程“揭榜挂帅”项目 (2022JBGS0014)
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ZHAO P P,SHAO W S,JIN C Q,et al. Effects of enclosure on biomass allocation characteristics of Agropyron mongolicum population in desert steppe[J]. Ecology and Environmental Sciences,2017,26(12):2024-2029赵盼盼,邵文山,靳长青,等. 围封对荒漠草原沙芦草种群构件生物量分配特性的影响[J]. 生态环境学报,2017,26(12):2024-2029 LI X Q,GAO Y H,LIU Y,et al. The genetic diversity of 9 populations of dry-desert Agropyron mongolicumcollected in northern China[J]. Acta Prataculturae Sinica,2016,25(3):77-85李晓全,高有汉,刘扬,等. 我国北方9份旱生—沙生植物蒙古冰草遗传多样性研究[J]. 草业学报,2016,25(3):77-85 ZHOU L L,FU B Z,XU D M,et al. Effects of salt stress on germination characteristics of Agropyron mongolicum and salt-tolerance evaluation[J]. Pratacultural Science,2015,32(8):1252-1259周璐璐,伏兵哲,许冬梅,等. 盐胁迫对沙芦草萌发特性影响及耐盐性评价[J]. 草业科学,2015,32(8):1252-1259 LIU X C,HU H J,TIAN Y,et al. Effects of exogenous silicon nanosilicon on seed germination and physiological indicators of Capsicum annuum seeds under salt stress[J]. Seed,2025,44(4):26-33刘小岑,胡海骏,田野,等. 外源纳米硅对盐胁迫下辣椒种子萌发及生理指标的影响[J]. 种子,2025,44(4):26-33 FRACETO L F,GRILLO R,DE MEDEIROS G A,et al. Nanotechnology in agriculture: which innovation potential does it have?[J]. Frontiers in Environmental Science,2016,4:20 SRIVASTAVA P,BRAGANÇA J,RAMANAN S R,et al. Synthesis of silver nanoparticles using haloarchaeal isolate Halococcus salifodinae BK3[J]. Extremophiles,2013,17(5):821-831 ANAND A,KUMARI A,THAKUR M,et al. Hydrogen peroxide signaling integrates with phytohormones during the germination of magnetoprimed tomato seeds[J]. Scientific Reports,2019,9:8814 CHUNG I M,REKHA K,RAJAKUMAR G,et al. Influence of silver nanoparticles on the enhancement and transcriptional changes of glucosinolates and phenolic compounds in genetically transformed root cultures of Brassica rapa ssp. Rapa[J]. Bioprocess and Biosystems Engineering,2018,41(11):1665-1677 YOU P,HE X Q. Recent progress in seed nanopriming research[J]. Pratacultural Science,2020,37(8):1548-1557尤沛,何学青. 种子纳米引发的研究进展[J]. 草业科学,2020,37(8):1548-1557 ELKHATIB E,ATTIA M,MAHDY A,et al. Priming with mango peels nanoparticles enhances seed germination of maize (Zea mays L.) under salt stress[J]. Alexandria Science Exchange Journal,2019,40(4):767-780 AFZAL S,SHARMA D,SINGH N K. Eco-friendly synthesis of phytochemical-capped iron oxide nanoparticles as nano-priming agent for boosting seed germination in rice (Oryza sativa L.)[J]. Environmental Science and Pollution Research,2021,28(30):40275-40287 ABOU-ZEID H M,ISMAIL G S M,ABDEL-LATIF S A. Influence of seed priming with ZnO nanoparticles on the salt-induced damages in wheat (Triticum aestivum L.) plants[J]. Journal of Plant Nutrition,2021,44(5):629-643 YUE J M,CONG X X,LI M L. Application of multispectral imaging technology in seed quality detection[J]. Seed,2021,40(10):129-135岳佳铭,丛晓翔,李曼莉. 多光谱成像技术在种子质量检测研究中的应用[J]. 种子,2021,40(10):129-135 ELMASRY G,MANDOUR N,WAGNER M H,et al. Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds[J]. Plant Methods,2019,15:24 ZHANG H,YAO X G,ZHANG X B,et al. Measurement of rice leaf chlorophyll and seed nitrogen contents by using multi-spectral imagine[J]. Chinese Journal of Rice Science,2008,22(5):555-558张浩,姚旭国,张小斌,等. 基于多光谱图像的水稻叶片叶绿素和籽粒氮素含量检测研究[J]. 中国水稻科学,2008,22(5):555-558 REN L,LIU W,LIU C H,et al. Non-destructive detection of Aspergillus ochraceus in corn based on multispectral imaging technology[J]. Journal of Chinese Institute of Food Science and Technology,2024,24(6):402-409任林,刘伟,刘长虹,等. 基于多光谱成像技术的玉米赭曲霉菌无损检测[J]. 中国食品学报,2024,24(6):402-409 WU H Z. Research on grape sweetness detection technology based on multi-spectral imaging technology[D]. Changchun:Changchun University of Science and Technology,2024:36-42吴虹璋. 基于多光谱成像技术葡萄甜度检测技术研究[D]. 长春:长春理工大学,2024:36-42 General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China. Grass seed testing procedures—Germination test: GB/T 2930.4—2017[S]. Beijing:Standards Press of China,2017:1-22国家质量监督检验检疫总局中国国家标准化管理委员会. 草种子检验规程发芽试验:GB/T 2930.4—2017[S]. 北京:中国标准出版社,2017:1-22 DUAN B B,WEI Z D,WANG L. Analyzing the impacts of cadmium alone and in co-existence with polypropylene microplastics on seed germination and seedling growth of maize[J]. Plastics Science and Technology,2024,52(8):83-88段冰冰,魏张东,王琳. 重金属镉单独或与聚丙烯微塑料共同暴露对玉米种子萌发及幼苗生长的影响研究[J]. 塑料科技,2024,52(8):83-88 CAKMAK I,MARSCHNER H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves[J]. Plant Physiology,1992,98(4):1222-1227 KAR M,MISHRA D. Catalase,peroxidase,and polyphenoloxidase activities during rice leaf senescence[J]. Plant Physiology,1976,57(2):315-319 HOSSAIN Z,LÓPEZ-CLIMENT M F,ARBONA V,et al. Modulation of the antioxidant system in Citrus under waterlogging and subsequent drainage[J]. Journal of Plant Physiology,2009,166(13):1391-1404 ARRIGONI O,DIPIERRO S,BORRACCINO G. Ascorbate free radical reductase,a key enzyme of the ascorbic acid system[J]. FEBS Letters,1981,125(2):242-244 NAKANO Y,ASADA K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J]. Plant and Cell Physiology,1981,22(5):867-880 SHEN Z B,PAN D F,WANG J L,et al. Effects of saline-alkaloid stress on seed germination and seedling growth of grasses[J]. Acta Agrestia Sinica,2012,20(5):914-920申忠宝,潘多锋,王建丽,等. 混合盐碱胁迫对5种禾草种子萌发及幼苗生长的影响[J]. 草地学报,2012,20(5):914-920 WANG R H,HU H Z,LIU L,et al. Effects of exogenous spermidine on seed germination of sugar beet under salt stress[J]. Journal of Anhui Agricultural Sciences,2025,53(8):129-132王仁汉,胡海洲,刘蕾,等. 亚精胺对盐胁迫下甜菜种子萌发的影响[J]. 安徽农业科学,2025,53(8):129-132 SUN Y F. Effects of nanopriming with iron oxide (Ⅲ) on seed germination and drought resistance of switchgrass (Panicum virgatum)[D]. Yangling:Northwest A & F University,2021:22-26孙运府. 纳米铁引发对柳枝稷种子萌发特性及抗旱性的影响[D]. 杨凌:西北农林科技大学,2021:22-26 ZHAO D Q,FANG Z W,TANG Y H,et al. Graphene oxide as an effective soil water retention agent can confer drought stress tolerance to Paeonia ostii without toxicity[J]. Environmental Science & Technology,2020,54(13):8269-8279 SONG H,GENG Q L,WU X,et al. The transcription factor MYC1 interacts with FIT to negatively regulate iron homeostasis in Arabidopsis thaliana[J]. The Plant Journal,2023,114(1):193-208 LEE C W,MAHENDRA S,ZODROW K,et al. Developmental phytotoxicity of metal oxide nanoparticles to Arabidopsis thaliana[J]. Environmental Toxicology and Chemistry,2010,29(3):669-675 DURAN N M,SAVASSA S M,DE LIMA R G,et al. X-ray spectroscopy uncovering the effects of Cu based nanoparticle concentration and structure on Phaseolus vulgaris germination and seedling development[J]. Journal of Agricultural and Food Chemistry,2017,65(36):7874-7884 SZÖLLÖSI R,MOLNÁR Á,KONDAK S,et al. Dual effect of nanomaterials on germination and seedling growth: stimulation vs. phytotoxicity[J]. Plants,2020,9(12):1745 YU Y. Research on nano-priming and film coating technology of oat seeds resistant to UV-B stress[D]. Beijing:China Agricultural University,2024:18-19于洋. 基于纳米引发的耐UV-B型燕麦种子包膜技术研究[D].北京:中国农业大学,2024:18-19 DAS K,ROYCHOUDHURY A. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants[J]. Frontiers in Environmental Science,2014,2:53 NADARAJAH K K. ROS homeostasis in abiotic stress tolerance in plants[J]. International Journal of Molecular Sciences,2020,21(15):5208 MAZHAR M W,ISHTIAQ M,MAQBOOL M,et al. Seed priming with Calcium oxide nanoparticles improves germination,biomass,antioxidant defence and yield traits of canola plants under drought stress[J]. South African Journal of Botany,2022,151:889-899 DAM P,PARET M L,MONDAL R,et al. Advancement of noble metallic nanoparticles in agriculture: A promising future[J]. Pedosphere,2023,33(1):116-128 SHETTY N,OLESEN M H,GISLUM R,et al. Use of partial least squares discriminant analysis on visible-near infrared multispectral image data to examine germination ability and germ length in spinach seeds[J]. Journal of Chemometrics,2012,26(8/9):462-466