Article(id=1309569662015336980, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.02.026, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1743609600000, receivedDateStr=2025-04-03, revisedDate=1747670400000, revisedDateStr=2025-05-20, acceptedDate=null, acceptedDateStr=null, onlineDate=1790155338051, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790155338051, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790155338051, creator=13701087609, updateTime=1790155338051, updator=13701087609, issue=Issue{id=1309569631556301196, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='2', pageStart='377', pageEnd='744', issueExtLink='null', onlineDate='null', pubDate='1771084800000', pubDateStr='2026-02-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790155330790, creator='13701087609', updateTime=1790156750858, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575588013244561, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575588017438866, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, language=CN, specialIssueTitle=, coverIllustrator=, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=643, endPage=652, ext={EN=ArticleExt(id=1309569662401212950, articleId=1309569662015336980, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Litter Addition on Soil Enzyme Activitie and Stoichiometric Ratio in Sagebrush Desert Grassland, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=This study investigated the effects of different addition levels (0, 2.5, 5.0, 7.5 g) of Seriphidium transiliense leaf litter on the enzyme activities and stoichiometric ratios of β-1,4-glucosidase (βG), N-acetyl-β-glucosaminidase (NAG), leucine aminopeptidase (LAP), and alkaline phosphatase (AP) in 100.0 g of soil from Sagebrush desert grassland during 320 d (T1), 410 d (T2), and 500 d (T3) of decomposition using an outdoor Mason jar incubation method. The results showed that enzyme activities generally decreased over the decomposition period. Specifically, the βG activity in the 5.0 g treatment at T2 was significantly higher than that at T1 and T3 by 12.16% and 20.53%, respectively (P<0.05). The NAG activity in the 7.5 g treatment at T1 was significantly higher than that at T2 and T3 by 19.19% and 19.10%, respectively (P<0.05). Enzyme activities initially decreased and then increased with increasing litter addition. At T3, the 7.5 g treatment significantly enhanced NAG activity while reducing LAP activity (P<0.05). The average soil enzyme stoichiometric ratios of C∶N, C∶P, and N∶P were 0.65, 0.91, and 1.41, respectively, with a C∶N∶P stoichiometric ratio of 1∶1.26∶1.05, with the vector angles less than 45°. In conclusion, soil enzyme activities and their stoichiometric ratios were co-regulated by both litter addition amount and decomposition stage, and were constrained by soil nitrogen limitation., authors=MA Peng1, ZHOU Lei1, SUN Zong-jiu1,2,3, Asitaiken·Julihaiti1, WEI Peng4, authorsList=MA Peng, ZHOU Lei, SUN Zong-jiu, Asitaiken·Julihaiti, WEI Peng, authorCompany=1 College of Grassland Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;
2 Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang 830052, China;
3 Key Laboratory of Grassland Resources and Ecology of Western Arid Region, Ministry of Education, Urumqi, Xinjiang 830052, China;
4 Xinjiang Uygyr Automomous Region Academy of Animal Science, Urumqi, Xinjiang 830052, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569662317326869, articleId=1309569662015336980, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=凋落物添加对蒿类荒漠草地土壤酶活性及其化学计量比的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=本研究以伊犁绢蒿(Seriphidium transiliense)叶凋落物和蒿类荒漠土壤为对象,采用梅森瓶室外培养法,探讨叶凋落物不同添加量(0,2.5,5.0,7.5 g)分解320 d(T₁)、410 d(T₂)、500 d(T₃)对100.0 g土壤β-1,4-葡萄糖苷酶(β-1,4-glucosidase,βG)、N乙酰-β-氨基葡萄糖苷酶(N acetyl-β-glucosamine enzymes,NAG)、亮氨酸氨基肽酶(Leucine aminopeptidase,LAP)、碱性磷酸酶(Alkaline phosphatase,AP)活性及其化学计量比的影响。结果表明:酶活性总体随凋落物分解时间的推移递减,其中5.0 g处理在T₂阶段βG活性显著高于T₁、T₃阶段12.16%,20.53%(P<0.05),7.5 g处理在T₁阶段NAG活性显著高于T₂、T₃阶段19.19%,19.10%(P<0.05);酶活性随添加量增加呈先降后升趋势,T₃阶段7.5 g处理显著增加NAG活性并降低LAP活性(P<0.05);土壤酶C∶N、C∶P、N∶P平均值分别为0.65,0.91,1.41,C∶N∶P化学计量比为1∶1.26∶1.05,矢量角度均小于45°。综上,土壤酶活性及其化学计量比受凋落物添加量与分解阶段共同调控,且受土壤氮限制。, authors=马鹏1, 周磊1, 孙宗玖1,2,3, 阿斯太肯·居力海提1, 魏鹏4, authorsList=马鹏, 周磊, 孙宗玖, 阿斯太肯·居力海提, 魏鹏, authorCompany=1 新疆农业大学草业学院, 新疆 乌鲁木齐 830052;
2 新疆草地资源与生态自治区重点实验室, 新疆 乌鲁木齐 830052;
3 西部干旱区草地资源与生态教育部重点实验室, 新疆 乌鲁木齐 830052;
4 新疆维吾尔自治区畜牧科学院, 新疆 乌鲁木齐 830052, correspAuthors=null, authorNote=马鹏(1998-),男,回族,甘肃平凉人,硕士研究生,主要从事草地资源与生态方面的研究,E-mail:2864347311@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=W4fl6QJvb2CmHr0U4xpiag==, pdfFileSize=1784419, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目 (32060408); 新疆维吾尔自治区重点实验室开放课题 (2022D04003); 新疆维吾尔自治区高校基本科研业务费 (XJEDU2022J006); 新疆维吾尔自治区自然科学基金 (2024D01A41))}, authors=null, keywords=[Keyword(id=1309569662548013591, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569662015336980, language=CN, orderNo=1, keyword=凋落物), Keyword(id=1309569662657065496, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569662015336980, 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草地学报 | 研究论文 2026,34(2): 643-652
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草地学报 |研究论文 2026 , 34 (2) : 643 -652
凋落物添加对蒿类荒漠草地土壤酶活性及其化学计量比的影响
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马鹏1, 周磊1, 孙宗玖1,2,3, 阿斯太肯·居力海提1, 魏鹏4
作者信息
    1 新疆农业大学草业学院, 新疆 乌鲁木齐 830052;
    2 新疆草地资源与生态自治区重点实验室, 新疆 乌鲁木齐 830052;
    3 西部干旱区草地资源与生态教育部重点实验室, 新疆 乌鲁木齐 830052;
    4 新疆维吾尔自治区畜牧科学院, 新疆 乌鲁木齐 830052
作者简介:
马鹏(1998-),男,回族,甘肃平凉人,硕士研究生,主要从事草地资源与生态方面的研究,E-mail:2864347311@qq.com
Effects of Litter Addition on Soil Enzyme Activitie and Stoichiometric Ratio in Sagebrush Desert Grassland
  • MA Peng1, ZHOU Lei1, SUN Zong-jiu1,2,3, Asitaiken·Julihaiti1, WEI Peng4
  • Affiliations
      1 College of Grassland Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;
      2 Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang 830052, China;
      3 Key Laboratory of Grassland Resources and Ecology of Western Arid Region, Ministry of Education, Urumqi, Xinjiang 830052, China;
      4 Xinjiang Uygyr Automomous Region Academy of Animal Science, Urumqi, Xinjiang 830052, China
    doi: 10.11733/j.issn.1007-0435.2026.02.026
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    本研究以伊犁绢蒿(Seriphidium transiliense)叶凋落物和蒿类荒漠土壤为对象,采用梅森瓶室外培养法,探讨叶凋落物不同添加量(0,2.5,5.0,7.5 g)分解320 d(T₁)、410 d(T₂)、500 d(T₃)对100.0 g土壤β-1,4-葡萄糖苷酶(β-1,4-glucosidase,βG)、N乙酰-β-氨基葡萄糖苷酶(N acetyl-β-glucosamine enzymes,NAG)、亮氨酸氨基肽酶(Leucine aminopeptidase,LAP)、碱性磷酸酶(Alkaline phosphatase,AP)活性及其化学计量比的影响。结果表明:酶活性总体随凋落物分解时间的推移递减,其中5.0 g处理在T₂阶段βG活性显著高于T₁、T₃阶段12.16%,20.53%(P<0.05),7.5 g处理在T₁阶段NAG活性显著高于T₂、T₃阶段19.19%,19.10%(P<0.05);酶活性随添加量增加呈先降后升趋势,T₃阶段7.5 g处理显著增加NAG活性并降低LAP活性(P<0.05);土壤酶C∶N、C∶P、N∶P平均值分别为0.65,0.91,1.41,C∶N∶P化学计量比为1∶1.26∶1.05,矢量角度均小于45°。综上,土壤酶活性及其化学计量比受凋落物添加量与分解阶段共同调控,且受土壤氮限制。
    凋落物  /  蒿类荒漠草地  /  化学计量比  /  土壤酶活性
    This study investigated the effects of different addition levels (0, 2.5, 5.0, 7.5 g) of Seriphidium transiliense leaf litter on the enzyme activities and stoichiometric ratios of β-1,4-glucosidase (βG), N-acetyl-β-glucosaminidase (NAG), leucine aminopeptidase (LAP), and alkaline phosphatase (AP) in 100.0 g of soil from Sagebrush desert grassland during 320 d (T1), 410 d (T2), and 500 d (T3) of decomposition using an outdoor Mason jar incubation method. The results showed that enzyme activities generally decreased over the decomposition period. Specifically, the βG activity in the 5.0 g treatment at T2 was significantly higher than that at T1 and T3 by 12.16% and 20.53%, respectively (P<0.05). The NAG activity in the 7.5 g treatment at T1 was significantly higher than that at T2 and T3 by 19.19% and 19.10%, respectively (P<0.05). Enzyme activities initially decreased and then increased with increasing litter addition. At T3, the 7.5 g treatment significantly enhanced NAG activity while reducing LAP activity (P<0.05). The average soil enzyme stoichiometric ratios of C∶N, C∶P, and N∶P were 0.65, 0.91, and 1.41, respectively, with a C∶N∶P stoichiometric ratio of 1∶1.26∶1.05, with the vector angles less than 45°. In conclusion, soil enzyme activities and their stoichiometric ratios were co-regulated by both litter addition amount and decomposition stage, and were constrained by soil nitrogen limitation.
    Litter  /  Sagebrush desert grassland  /  Stoichiometric ratio  /  Soil enzyme activitie
    马鹏, 周磊, 孙宗玖, 阿斯太肯·居力海提, 魏鹏. 凋落物添加对蒿类荒漠草地土壤酶活性及其化学计量比的影响. 草地学报, 2026 , 34 (2) : 643 -652 . DOI: 10.11733/j.issn.1007-0435.2026.02.026
    MA Peng, ZHOU Lei, SUN Zong-jiu, Asitaiken·Julihaiti, WEI Peng. Effects of Litter Addition on Soil Enzyme Activitie and Stoichiometric Ratio in Sagebrush Desert Grassland[J]. Acta Agrestia Sinica, 2026 , 34 (2) : 643 -652 . DOI: 10.11733/j.issn.1007-0435.2026.02.026

      国家自然科学基金项目 (32060408); 新疆维吾尔自治区重点实验室开放课题 (2022D04003); 新疆维吾尔自治区高校基本科研业务费 (XJEDU2022J006); 新疆维吾尔自治区自然科学基金 (2024D01A41)

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    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
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