Article(id=1309569652532015590, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.02.013, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1741622400000, receivedDateStr=2025-03-11, revisedDate=1744041600000, revisedDateStr=2025-04-08, acceptedDate=null, acceptedDateStr=null, onlineDate=1790155335790, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790155335790, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790155335790, creator=13701087609, updateTime=1790155335790, updator=13701087609, issue=Issue{id=1309569631556301196, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='2', pageStart='377', pageEnd='744', issueExtLink='null', onlineDate='null', pubDate='1771084800000', pubDateStr='2026-02-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790155330790, creator='13701087609', updateTime=1790156750858, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575588013244561, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575588017438866, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, language=CN, specialIssueTitle=, coverIllustrator=, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=509, endPage=517, ext={EN=ArticleExt(id=1309569652922085864, articleId=1309569652532015590, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Growth and Physiological Responses of ‘Zhongmu No.3’ Alfalfa Cultivar to Different Saline-Alkali Stresses, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To investigate the growth and metabolic adaptability of alfalfa (Medicago sativa L.) under saline-alkali soil conditions, this experiment used ‘Zhongmu No.3’ alfalfa cuttings as the test material. Set up neutral salt stress (NaCl+Na2SO4) and alkaline salt stress (Na2CO3+NaHCO3) treatments, with three gradient levels for each: light (0.3% soil salinity), moderate (0.6%), and severe (0.9%). After 15 days of stress, the growth physiological indicators of plant leaves and roots were measured. The results showed that alfalfa exhibited significant growth and physiological responses under saline-alkali stress. With increasing stress intensity, growth parameters under neutral salt treatment reached the lowest level under severe stress treatment (NS), whereas alkaline salt treatment exhibited a gradual decrease in growth parameters as treatment intensity escalated. The sodium ion (Na+) content in the leaves and roots significantly increased under different treatments. The content of malondialdehyde (MDA) in the leaves significantly rose under moderate (NM) and NS neutral salt stress treatments; meanwhile, the MDA content in both roots and leaves significantly increased under various alkaline salt stresses. The proline (Pro) content in the roots significantly increased under different stresses, while the activity of superoxide dismutase (SOD) first increased and then decreased as the stress level rose. This study reveals the growth and metabolic adaptation mechanisms of alfalfa under saline-alkali stress, providing research references for the breeding of salt-tolerant varieties., authors=GUO Mei-ying1, LIU Ya-ling2, LI Xiao-xia3, LIU Xi-qiang3, ZHAO Yan1, authorsList=GUO Mei-ying, LIU Ya-ling, LI Xiao-xia, LIU Xi-qiang, ZHAO Yan, authorCompany=1 College of Grassland Science, Inner Mongolia Agricultural University, Key Laboratory of Grassland Resources (IMAU), Hohhot, Inner Mongolia 010010, China;
2 Inner Mongolia Pratacultural Technology Innovation Center Co., Ltd, Hohhot, Inner Mongolia 010070, China;
3 Institute of Ecological Protection and Restoration, Chinese Academy of Forestry/Grassland Research Center, National Forestry and Grassland Administration, Beijing 100091, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569652838199783, articleId=1309569652532015590, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=‘中苜3号’紫花苜蓿对不同盐碱胁迫的生长和生理响应, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为探究紫花苜蓿在盐碱土壤环境下的生长和代谢适应性,本试验以‘中苜3号’紫花苜蓿扦插苗为试验材料,设置中性盐(Neutral salt stress,NaCl+Na₂SO₄)和碱性盐(Alkaline salt stress,Na₂CO₃+NaHCO₃)胁迫处理,分别设置轻度(0.3%土壤含盐量,Light)、中度(0.6%,Moderate)和重度(0.9%,Severe)三个梯度处理。胁迫15天后测定植株叶和根的生长生理指标。结果表明,紫花苜蓿在盐碱胁迫下表现出显著的生长和生理响应。随着胁迫程度增加,中性盐处理下的生长指标在重度胁迫处理(NS)下最低,碱性盐处理下随着处理程度的增加逐渐降低。叶和根中的钠离子(Na⁺)含量在不同处理下均显著增加,叶中丙二醛(Malondialdehyde,MDA)含量在中性盐中度胁迫处理(NM)和NS下显著升高;根和叶中的MDA含量在不同碱性盐胁迫下均显著增加。根中脯氨酸(Proline,Pro)含量在不同胁迫下均显著升高,超氧化物歧化酶(Superoxide dismutase,SOD)活性随胁迫程度增加呈先升高后降低的变化。本研究揭示了紫花苜蓿在盐碱胁迫下的生长和代谢适应机制,为耐盐碱品种选育提供了研究参考。, authors=郭美莹1, 刘亚玲2, 李晓霞3, 刘希强3, 赵彦1, authorsList=郭美莹, 刘亚玲, 李晓霞, 刘希强, 赵彦, authorCompany=1 内蒙古农业大学草业学院/草地资源教育部重点实验室, 内蒙古 呼和浩特 010010;
2 内蒙古草业技术创新中心有限公司, 内蒙古 呼和浩特 010070;
3 中国林业科学研究院生态保护与修复研究所/国家林业和草原局草原研究中心, 北京 100091, correspAuthors=null, authorNote=郭美莹(1999-),女,汉族,内蒙古锡林郭勒人,硕士研究生,主要从事草种质资源与育种研究,E-mail:15847769348@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=p9Knn10/lDZMwvT8JKtJGw==, pdfFileSize=1386727, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=农业生物育种重大专项 (2022ZD0401705); 2023年国家草业技术创新中心(筹)重大创新平台建设专项 (CCPTZX2023W01); 2023年呼和浩特市“揭榜挂帅”项目 (2023-揭榜挂帅-社-1))}, authors=[Author(id=1320578160740118661, tenantId=1146029695717560320, journalId=null, articleId=1309569652532015590, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, 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detailUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/10.11733/j.issn.1007-0435.2026.02.013, pdfUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/PDF/10.11733/j.issn.1007-0435.2026.02.013, pdfUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/PDF/10.11733/j.issn.1007-0435.2026.02.013, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1771084800000, fullTextJson=null, articleText=null, reference=YANG J S, YAO R J, WANG X P, et al. 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WANG H,TAKANO T,LIU S K. Screening and evaluation of saline-alkaline tolerant germplasm of rice (Oryza sativa L.) in soda saline-alkali soil[J]. Agronomy,2018,8(10):205
ZHANG H L,YU F F,XIE P, et al. A Gγ protein regulates alkaline sensitivity in crops[J]. Science,2023,379(6638):eade8416
ZHU J K. Abiotic stress signaling and responses in plants[J]. Cell,2016,167(2):313-324
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SKAGGS R W,SCHILFGAARDE J V,OSTER J D,et al. Reclamation of salt affected soils[M]. Wageningen:Agricultural Drainage,1999:65
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WANG X C,YANG T H,WANG C,et al. Effects of mixed saline-alkali stress on growth and physiology of Alfalfa[J]. Chinese Agricultural Science Bulletin,2022,38(19):139-145 王晓春,杨天辉,王川,等. 混合盐碱胁迫对紫花苜蓿的生长和生理指标的影响[J]. 中国农学通报,2022,38(19):139-145
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ZHANG Y F,YIN B. Influences of salt and alkali mixed stresses on antioxidative activity and MDA content of Medicago sativa at seedling stage[J]. Acta Prataculturae Sinica,2009,18(1):46-50 张永峰,殷波. 混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响[J]. 草业学报,2009,18(1):46-50
ZHU C C,SHI K,HE Q K,et al. Effects of mixed saline-alkali stress on physiology and gene expression of Alfalfa seedlings[J]. Acta Agrestia Sinica,2024,32(4):1044-1054 朱晨晨,史昆,何沁坤,等. 混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响[J]. 草地学报, 2024, 32(4):1044-1054
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‘中苜3号’紫花苜蓿对不同盐碱胁迫的生长和生理响应
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郭美莹, 刘亚玲, 李晓霞, 刘希强, 赵彦
草地学报 | 研究论文 2026,34(2): 509-517
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草地学报 |研究论文 2026 , 34 (2) : 509 -517
‘中苜3号’紫花苜蓿对不同盐碱胁迫的生长和生理响应
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郭美莹, 刘亚玲, 李晓霞, 刘希强, 赵彦
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作者简介:
郭美莹(1999-),女,汉族,内蒙古锡林郭勒人,硕士研究生,主要从事草种质资源与育种研究,E-mail:15847769348@163.com
Growth and Physiological Responses of ‘Zhongmu No.3’ Alfalfa Cultivar to Different Saline-Alkali Stresses
GUO Mei-ying, LIU Ya-ling, LI Xiao-xia, LIU Xi-qiang, ZHAO Yan
Affiliations
    1 College of Grassland Science, Inner Mongolia Agricultural University, Key Laboratory of Grassland Resources (IMAU), Hohhot, Inner Mongolia 010010, China;
    2 Inner Mongolia Pratacultural Technology Innovation Center Co., Ltd, Hohhot, Inner Mongolia 010070, China;
    3 Institute of Ecological Protection and Restoration, Chinese Academy of Forestry/Grassland Research Center, National Forestry and Grassland Administration, Beijing 100091, China
doi: 10.11733/j.issn.1007-0435.2026.02.013
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为探究紫花苜蓿在盐碱土壤环境下的生长和代谢适应性,本试验以‘中苜3号’紫花苜蓿扦插苗为试验材料,设置中性盐(Neutral salt stress,NaCl+Na₂SO₄)和碱性盐(Alkaline salt stress,Na₂CO₃+NaHCO₃)胁迫处理,分别设置轻度(0.3%土壤含盐量,Light)、中度(0.6%,Moderate)和重度(0.9%,Severe)三个梯度处理。胁迫15天后测定植株叶和根的生长生理指标。结果表明,紫花苜蓿在盐碱胁迫下表现出显著的生长和生理响应。随着胁迫程度增加,中性盐处理下的生长指标在重度胁迫处理(NS)下最低,碱性盐处理下随着处理程度的增加逐渐降低。叶和根中的钠离子(Na⁺)含量在不同处理下均显著增加,叶中丙二醛(Malondialdehyde,MDA)含量在中性盐中度胁迫处理(NM)和NS下显著升高;根和叶中的MDA含量在不同碱性盐胁迫下均显著增加。根中脯氨酸(Proline,Pro)含量在不同胁迫下均显著升高,超氧化物歧化酶(Superoxide dismutase,SOD)活性随胁迫程度增加呈先升高后降低的变化。本研究揭示了紫花苜蓿在盐碱胁迫下的生长和代谢适应机制,为耐盐碱品种选育提供了研究参考。
紫花苜蓿  /  盐碱胁迫  /  生长指标  /  生理响应
To investigate the growth and metabolic adaptability of alfalfa (Medicago sativa L.) under saline-alkali soil conditions, this experiment used ‘Zhongmu No.3’ alfalfa cuttings as the test material. Set up neutral salt stress (NaCl+Na2SO4) and alkaline salt stress (Na2CO3+NaHCO3) treatments, with three gradient levels for each: light (0.3% soil salinity), moderate (0.6%), and severe (0.9%). After 15 days of stress, the growth physiological indicators of plant leaves and roots were measured. The results showed that alfalfa exhibited significant growth and physiological responses under saline-alkali stress. With increasing stress intensity, growth parameters under neutral salt treatment reached the lowest level under severe stress treatment (NS), whereas alkaline salt treatment exhibited a gradual decrease in growth parameters as treatment intensity escalated. The sodium ion (Na+) content in the leaves and roots significantly increased under different treatments. The content of malondialdehyde (MDA) in the leaves significantly rose under moderate (NM) and NS neutral salt stress treatments; meanwhile, the MDA content in both roots and leaves significantly increased under various alkaline salt stresses. The proline (Pro) content in the roots significantly increased under different stresses, while the activity of superoxide dismutase (SOD) first increased and then decreased as the stress level rose. This study reveals the growth and metabolic adaptation mechanisms of alfalfa under saline-alkali stress, providing research references for the breeding of salt-tolerant varieties.
Alfalfa  /  Saline-alkali stress  /  Growth traits  /  Physiological response
郭美莹, 刘亚玲, 李晓霞, 刘希强, 赵彦. ‘中苜3号’紫花苜蓿对不同盐碱胁迫的生长和生理响应. 草地学报, 2026 , 34 (2) : 509 -517 . DOI: 10.11733/j.issn.1007-0435.2026.02.013
GUO Mei-ying, LIU Ya-ling, LI Xiao-xia, LIU Xi-qiang, ZHAO Yan. Growth and Physiological Responses of ‘Zhongmu No.3’ Alfalfa Cultivar to Different Saline-Alkali Stresses[J]. Acta Agrestia Sinica, 2026 , 34 (2) : 509 -517 . DOI: 10.11733/j.issn.1007-0435.2026.02.013

    农业生物育种重大专项 (2022ZD0401705); 2023年国家草业技术创新中心(筹)重大创新平台建设专项 (CCPTZX2023W01); 2023年呼和浩特市“揭榜挂帅”项目 (2023-揭榜挂帅-社-1)

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Food and Agriculture Organization of the United Nations (FAO). Status of World Soil Resources: Main Report [EB/OL]. https://www.fao.org/3/i5199e/i5199e.pdf, 2015-10-15
WANG H,TAKANO T,LIU S K. Screening and evaluation of saline-alkaline tolerant germplasm of rice (Oryza sativa L.) in soda saline-alkali soil[J]. Agronomy,2018,8(10):205
ZHANG H L,YU F F,XIE P, et al. A Gγ protein regulates alkaline sensitivity in crops[J]. Science,2023,379(6638):eade8416
ZHU J K. Abiotic stress signaling and responses in plants[J]. Cell,2016,167(2):313-324
LI J W. The mechanism of Oat response to salt stress and alk[D]. Hohhot: Inner Mongolia Agricultural University, 2023:1-2 李俊伟. 燕麦对盐胁迫和碱胁迫的响应机制[D]. 呼和浩特: 内蒙古农业大学,2023:1-2
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LI Q,ZHOU D W. Effect of grassland management on soil carbon sequestration[J]. Journal of Anhui Agricultural Sciences,2018,46(23):1-5,8 李强,周道玮. 草地管理对土壤碳截获的影响[J]. 安徽农业科学,2018,46(23):1-5,8
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LIU D,BAI S,YANG Q S,et al. A review on the saline-alkaline tolerance of alfalfa (Medicago sativa L.) [J]. Journal of Biology,2021,38(1):98-101,105 刘铎,白爽,杨庆山,等. 紫花苜蓿(Medicago sativa L.)耐盐碱研究进展[J]. 生物学杂志,2021,38(1):98-101,105
YANG Q C,SUN J,KANG J M,et al. Breeding and application of a new salt-tolerant alfalfa variety ‘Zhongmu No. 3’ [Z]. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences Institute of Animal Science,Chinese Academy of Agricultural Sciences,2019-10-10 杨青川,孙杰,康俊梅,等. 耐盐苜蓿“中苜3号”新品种的选育与应用[Z]. 内蒙古自治区农牧业科学院中国农业科学院北京畜牧兽医研究所,2019-10-10
ZHANG Y G,ZHANG Y,WANG C,et al. Enhancement of salt tolerance of alfalfa: physiological and molecular responses of transgenic alfalfa plants expressing Syntrichia caninervis-derived ScABI3[J]. Plant Physiology and Biochemistry,2024(207):108335
WANG X C,YANG T H,WANG C,et al. Effects of mixed saline-alkali stress on growth and physiology of Alfalfa[J]. Chinese Agricultural Science Bulletin,2022,38(19):139-145 王晓春,杨天辉,王川,等. 混合盐碱胁迫对紫花苜蓿的生长和生理指标的影响[J]. 中国农学通报,2022,38(19):139-145
JIA X F,LI B. Effects of NaHCO3+Na2CO3 combined saline alkali stress on physiological metabolism of alfalfa seedlings[J]. Heilongjiang Animal Science and Veterinary Medicine,2021(23):99-103 贾秀峰,李波. NaHCO3+Na2CO3复合盐碱胁迫对苜蓿幼苗生理代谢的影响[J]. 黑龙江畜牧兽医,2021(23):99-103
ZHANG Y F,YIN B. Influences of salt and alkali mixed stresses on antioxidative activity and MDA content of Medicago sativa at seedling stage[J]. Acta Prataculturae Sinica,2009,18(1):46-50 张永峰,殷波. 混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响[J]. 草业学报,2009,18(1):46-50
ZHU C C,SHI K,HE Q K,et al. Effects of mixed saline-alkali stress on physiology and gene expression of Alfalfa seedlings[J]. Acta Agrestia Sinica,2024,32(4):1044-1054 朱晨晨,史昆,何沁坤,等. 混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响[J]. 草地学报, 2024, 32(4):1044-1054
HOU Z,ZHAO L,WANG Y,et al. Purification and characterization of superoxide dismutases from sea buckthorn and chestnut rose[J]. Journal of food science,2019,84(4):746-753
VIEIRA S M,SILVA T M,GLÓRIA M B A. Influence of processing on the levels of amines and proline and on the physico-chemical characteristics of concentrated orange juice[J]. Food chemistry,2010,119(1):7-11
National Health Commission. GB 5009.268-2016 National food safety standard-determination of multiple elements in foods[S]. Beijing: China Standards Press, 2016:16 国家卫生健康委员会. GB 5009.268-2016 食品安全国家标准食品中多元素的测定[S]. 北京:中国标准出版社,2016:16
YU H R,JIA Y S,JIA P F,et al. Comprehensive evaluation of growth, yield and quality of alfalfa in different saline-alkali soil[J]. Chinese Journal of Grassland,2019,41(4):143-149 于浩然,贾玉山,贾鹏飞,等. 不同盐碱度对紫花苜蓿产量及品质的影响[J]. 中国草地学报,2019,41(4):143-149
FANG S,HOU X,LIANG X. Response mechanisms of plants under saline-alkali stress[J]. Frontiers in Plant Science,2021,4(12):667458
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2026年第34卷第2期
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doi: 10.11733/j.issn.1007-0435.2026.02.013
  • 接收时间:2025-03-11
  • 首发时间:2026-09-23
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  • 收稿日期:2025-03-11
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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