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Salt-treated stolons of P. vaginatum ‘Sea Isle 2000’ were used as experimental material. The promoter sequence of Pvrboh5 was amplified by PCR and subsequently ligated into the yeast one-hybrid bait vector pBait-AbAi， the construct was named as pPvrboh5-AbAi. This construct was then transformed into the Y1H Gold yeast strain to generate the recombinant bait strain. Concurrently， the yeast one-hybrid library was constructed using Gateway technology. Following mRNA isolation and purification， three reading-frame double-stranded cDNAs were synthesized. Primary libraries were constructed via BP reaction， followed by the generation of high-quality secondary libraries through LR reaction. Finally， transcription factors interacting with Pvrboh5 were identified by screening the P. vaginatum yeast cDNA library. The results showed that transcriptional autoactivation of the pPvrboh5-AbAi/Y1H Gold bait strain was effectively suppressed at an AbA concentration of 100 ng·mL^-1. In the screening experiment， a total of 18 proteins that bind to the Pvrboh5 promoter were identified. These findings provide a theoretical foundation for further elucidating the regulatory mechanisms of the Pvrboh5 gene., authors=LU Yang¹, CAI Li-rong¹, PENG Di¹, CHEN Ya-fang¹, LI Jun-xian¹, LIU Da-lin¹, PAN Ling¹, authorsList=LU Yang, CAI Li-rong, PENG Di, CHEN Ya-fang, LI Jun-xian, LIU Da-lin, PAN Ling, authorCompany=1 Yangzhou University, College of Animal Science and Technology, Yangzhou, Jiangsu Province 225009, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569636270703289, articleId=1309569635960324792, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=海雀稗酵母单杂文库构建及Pvrboh5上游调控因子筛选, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为了探究Pvrboh5表达的分子调控机制，本研究运用gateway技术构建了海雀稗 （Paspalum vaginatum Swartz）酵母单杂文库并筛选海雀稗Pvrboh5的上游调控因子。本试验以盐处理‘海岛2000’海雀稗为材料，经PCR扩增得到Pvrboh5的启动子序列，再将其连接至酵母单杂诱饵载体pBait-AbAi上并命名为pPvrboh5-AbAi，接着转化到Y1H Gold酵母菌株上构建重组诱饵菌株。同时利用gateway技术构建酵母单杂文库。在分离纯化mRNA的基础上，合成了3种读码框的双链cDNA，通过BP反应构建其相应的初级文库，再通过LR反应构建高质量的次级文库。最后从海雀稗酵母cDNA文库中筛选与Pvrboh5互作的转录因子。结果显示，pPvrboh5-AbAi/Y1H Gold诱饵菌株在AbA浓度为100 ng·mL⁻¹时转录自激活得到有效抑制；在筛库实验中，共获得18个与Pvrboh5启动子结合的蛋白。以上结果为后续深入探究Pvrboh5基因调控机制提供理论基础。, authors=陆洋¹, 蔡丽蓉¹, 彭迪¹, 陈亚方¹, 黎俊显¹, 刘大林¹, 潘玲¹, authorsList=陆洋, 蔡丽蓉, 彭迪, 陈亚方, 黎俊显, 刘大林, 潘玲, authorCompany=1 扬州大学动物科学与技术学院, 江苏 扬州 225009, correspAuthors=null, authorNote=陆洋（2001-），男，汉族，江苏无锡人，硕士研究生，主要从事海雀稗机理研究，E-mail:lyyzdx2019@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, 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Research progress on stress resistance of seashore Paspalum[J]. Modern Agricultural Science and Technology，2024（11）：126-132 宋鑫. 海滨雀稗抗逆性研究进展[J]. 现代农业科技，2024（11）：126-132
QI P，EUDY D，SCHNABLE J C，et al. High density genetic maps of seashore Paspalum Using genotyping-by-sequencing and their relationship to the sorghum bicolor genome[J]. Scientific Reports，2019（9）：12183
SUN G C，WASE N，SHU S Q，et al. Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass[J]. Nature Communications，2022（13）：7731
SACHDEV S，ANSARI S A，ANSARI M I，et al. Abiotic stress and reactive oxygen species： generation，signaling，and defense mechanisms[J]. Antioxidants，2021，10（2）：277
JIANG X C，GONG J Y，GENG X M，et al. Study on the function and regulation mechanism of Rbohs gene in plants[J]. Journal of Biology，2025，42（3）：100-107 蒋序春，龚记熠，耿兴敏，等. 植物Rbohs基因功能及其调控机制的研究进展[J]. 生物学杂志，2025，42（3）：100-107
YANG C Y，HUANG Y C，OU S L. ERF73/HRE1 is involved in H₂O₂ production via hypoxia-inducible Rboh gene expression in hypoxia signaling[J]. Protoplasma，2017，254（4）：1705-1714
LIU D，LI Y Y，ZHOU Z C，et al. Tobacco transcription factor bHLH123 improves salt tolerance by activating NADPH oxidase NtRbohE expression[J]. Plant Physiology，2021，186（3）：1706-1720
TORRES M A，DANGL J L，JONES J D G. Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response[J]. PNAS，2002，99（1）：517-522
LI Y R，ZHOU L Y，DONG D，et al. Construction and analysis of a yeast hybrid cDNA library of Zoysia japonica[J]. Acta Agrestia Sinica，2023，31（11）：3271-3277 李艳茹，周丽莹，董笛，等. 日本结缕草（Zoysia japonica）酵母杂交cDNA文库的构建及分析[J]. 草地学报，2023，31（11）：3271-3277
FENG Z J，LIU N，ZHANG G W，et al. Construction of vegetable pea yeast one-hybrid cDNA library and sugar transporter PsSWEET15 bait vector[J]. Molecular Plant Breeding，2023，21（19）：6390-6397 冯志娟，刘娜，张古文，等. 菜用豌豆酵母单杂交cDNA文库及糖转运蛋白PsSWEET15诱饵载体构建[J]. 分子植物育种，2023，21（19）：6390-6397
HUANG X L，SUN G L，MA D D，et al. Construction of yeast one-hybrid library and screening of factors regulating LAZY1 expression in rice[J]. Biotechnology Bulletin，2023，39（9）：126-135 黄小龙，孙贵连，马丹丹，等. 水稻幼苗酵母单杂文库构建及LAZY1上游调控因子筛选[J]. 生物技术通报，2023，39（9）：126-135
OHARA O，TEMPLE G. Directional cDNA library construction assisted by the in vitro recombination reaction[J]. Nucleic Acids Research，2001，29（4）：e22
QIU A L，LIU L L，CAI H Y，et al. Construction of a cDNA library of pepper for yeast two-hybrid analysis using gateway technology[J]. Acta Agriculturae Universitatis Jiangxiensis，2010，32（4）：791-796 邱爱连，刘林林，蔡汉阳，等. Gateway技术构建酵母双杂分析用辣椒cDNA文库[J]. 江西农业大学学报，2010，32（4）：791-796
PAN L，XU T C，WANG J，et al. Root-sourced H₂O₂ is essential for maintaining jasmonic acid and Na+/K+ homeostasis to delay leaf senescence during salt stress in Paspalum vaginatum[J]. Horticultural Plant Journal，2025，11（3）：1364-1376
SAGI M，FLUHR R. Production of reactive oxygen species by plant NADPH oxidases[J]. Plant Physiology，2006，141（2）：336-340
WANG G F，LI W Q，LI W Y，et al. Characterization of rice NADPH oxidase genes and their expression under various environmental conditions[J]. International Journal of Molecular Sciences，2013，14（5）：9440-9458
YU S Z，KAKAR K U，YANG Z X，et al. Systematic study of the stress-responsive Rboh gene family in Nicotiana tabacum： genome-wide identification，evolution and role in disease resistance[J]. Genomics，2020，112（2）：1404-1418
LUO X F，DAI Y J，ZHENG C，et al. The ABI4-RbohD/VTC2 regulatory module promotes reactive oxygen species （ROS） accumulation to decrease seed germination under salinity stress[J]. New Phytologist，2021，229（2）：950-962
BONCHUK A，BALAGUROV K，GEORGIEV P. BTB domains： A structural view of evolution，multimerization，and protein-protein interactions[J]. BioEssays，2023，45（2）：2200179
ZHANG S Z，YAN X F，ZHANG C Z，et al. Progress in the studies of plant BTB/POZ protein and the disease resistance[J]. Soybean Science，2019，38（2）：311-316 张淑珍，闫晓飞，张传忠，等. 植物BTB/POZ蛋白及其抗病性研究进展[J]. 大豆科学，2019，38（2）：311-316
CHERN M，FITZGERALD H A，CANLAS P E，et al. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light[J]. Molecular Plant-Microbe Interactions，2005，18（6）：511-520
SILVA K J P，BRUNINGS A，PERES N A，et al. The Arabidopsis NPR1 gene confers broad-spectrum disease resistance in strawberry[J]. Transgenic Research，2015，24（4）：693-704
ZHOU F，WANG Y G，LIU P F，et al. Function of ZmBT2agene in resistance to pathogen infection in maize[J]. Phytopathology Research，2024，6（1）：43
OOKA H，SATOH K，DOI K，et al. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana[J]. DNA Research，2003，10（6）：239-247
TRAN L P，NAKASHIMA K，SAKUMA Y，et al. Isolation and functional analysis of Arabidopsis stress-inducible-inducible NAC transcription factors that bind to a drought-responsive Cis-element in the early responsive to dehydration stress 1 promoter[J]. The Plant Cell，2004，16（9）：2481-2498
YU J Y，GONG C R. Advances in biological functions of plant NAC transcription factors[J]. Henan Science，2024，42（11）：1589-1601 于婧瑶，巩琛锐. 植物NAC转录因子的生物学功能研究进展[J]. 河南科学，2024，42（11）：1589-1601
WANG C，SONG S W，FU J，et al. The transcription factor OsNAC25 regulates potassium homeostasis in rice[J]. Plant Biotechnology Journal，2025，23（3）：930-945
XU P P，MA W，FENG H F，et al. The NAC056 transcription factor confers freezing tolerance by positively regulating expression of CBFs and NIA1 in Arabidopsis[J]. Plant Communications，2024，5（7）：100923
PODZIMSKA-SROKA D，O’SHEA C，GREGERSEN P L，et al. NAC transcription factors in senescence： from molecular structure to function in crops[J]. Plants，2015，4（3）：412-448
MATALLANA-RAMIREZ L P，RAUF M，FARAGE-BARHOM S，et al. NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 （BFN1） constitute a regulatory cascade in Arabidopsis[J]. Molecular Plant，2013，6（5）：1438-1452
ZHANG Y Y，ZANG Y H，CHEN J W，et al. A truncated ETHYLENE INSENSITIVE3-like protein，GhLYI，regulates senescence in cotton[J]. Plant Physiology，2023，193（2）：1177-1196
JUNG K，GIL N，OK L. Regulatory network of NAC transcription factors in leaf senescence[J]. Current Opinion in Plant Biology，2016，33：48-56
BALAZADEH S，KWASNIEWSKI M，CALDANA C，et al. ORS1，an H₂O₂-responsive NAC transcription factor，controls senescence in Arabidopsis thaliana[J]. Molecular Plant，2011，4（2）：346-360
BALAZADEH S，SIDDIQUI H，ALLU A D，et al. A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence[J]. The Plant Journal，2010，62（2）：250-264)