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To screen low phosphorus tolerant alfalfa varieties with high phosphorus use efficiency, we measured the aboveground and belowground biomass and root morphological indices for the 31 alfalfa varieties. The results indicated that the aboveground and belowground biomass of alfalfa were lowest under P5 treatment. With increasing phosphorus supply, the aboveground biomass of alfalfa significantly increased, reaching its highest level under the P50 treatment. Under the P20 and P50 treatments, the root biomass of alfalfa was significantly higher than that under P5, but there was no significant difference between P20 and P50. However, the root morphology showed significant variation among different varieties. Among them, ‘HD’‘Algonguin’‘Magna Graze II’ and ‘Longmu 801’ had higher biomass than other varieties, and exhibited greater root morphological plasticity with higher root length, root volume, root surface area, and root diameter, which demonstrated tolerance to low phosphorus condition. The results provide a scientific basis for optimizing alfalfa cultivation and improving phosphorus fertilizer utilization efficiency., authors=FENG Zi-xuan1, XU Zi-ying1, MA Hong-yan1, JING Jing-ying1, authorsList=FENG Zi-xuan, XU Zi-ying, MA Hong-yan, JING Jing-ying, authorCompany=1 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569636052595099, articleId=1309569635771576730, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=磷添加对不同品种苜蓿生物量及根系可塑性的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为研究苜蓿根系形态的可塑性对不同磷供应强度的响应,选取30个不同的紫花苜蓿(Medicago sativa L.)品种和1个黄花苜蓿(Medicago falcata L.)品种,分别设置P₅(5 mg·kg⁻¹ KH₂PO₄)、P₂₀(20 mg·kg⁻¹ KH₂PO₄)和P₅₀(50 mg·kg⁻¹ KH₂PO₄)三个水平的磷添加处理,通过测定31个品种苜蓿的地上、地下生物量及根系形态指标,明确不同品种的苜蓿响应不同磷添加水平的差异,以期筛选出耐低磷的磷高效苜蓿品种。结果表明,P₅处理下苜蓿地上和地下生物量最低,随着供磷量的增加,苜蓿的地上生物量显著提高,在P₅₀处理下最高。在P₂₀和P₅₀处理下,苜蓿的根系生物量显著高于P₅处理,而根系形态在不同品种间差异显著。其中 ‘旱地’‘阿尔冈金’‘Magna Graze II’‘龙牧801’ 在低磷水平下较其他品种生物量高,且根长、根体积、根表面积以及根直径表现出更强的可塑性,为极耐低磷的磷高效品种。研究结果为苜蓿的合理栽培、磷肥利用率的提高提供科学依据。, authors=冯恣萱1, 许紫莹1, 马红艳1, 荆晶莹1, authorsList=冯恣萱, 许紫莹, 马红艳, 荆晶莹, authorCompany=1 中国农业大学草业科学与技术学院, 北京 100193, correspAuthors=null, authorNote=冯恣萱(2002-),女,汉族,四川成都人,硕士研究生,主要从事草地生产与利用研究,E-mail:15228827090@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=CocXzeNAaPuQdsHR4JG1UQ==, 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REICHERT T,RAMMIG A,FUCHSLUEGER L,et al. Plant phosphorus-use and-acquisition strategies in Amazonia[J]. New Phytologist,2022,234(4):1126-1143
YU Q S,NI X F,CHENG X L,et al. Foliar phosphorus allocation and photosynthesis reveal plants' adaptative strategies to phosphorus limitation in tropical forests at different successional stages[J]. Science of The Total Environment,2022,846:157456
BERG W K,CUNNINGHAM S M,BROUDER S M,et al. Influence of phosphorus and potassium on alfalfa yield and yield components[J]. Crop Science,2005,45(1):297-304
RICHARDSON A E,SIMPSON R J. Soil microorganisms mediating phosphorus availability update on microbial phosphorus[J]. Plant Physiology,2011,156(3):989-996
SUN B R,GAO Y Z,WU X,et al. The relative contributions of pH,organic anions,and phosphatase to rhizosphere soil phosphorus mobilization and crop phosphorus uptake in maize/alfalfa polyculture[J]. Plant and Soil,2020,447(1):117-133
PAN X Y,LI J B,CHEN Y,et al. Response of root morphology and anatomical structure of six alfalfa cultivars to phosphorus deficiency[J]. Acta Agrestia Sinica,2021,29(11):2494-2504 潘新雅,李军保,陈阳,等. 6个紫花苜蓿品种根系形态结构对低磷胁迫的响应[J]. 草地学报,2021, 29(11):2494-2504
MA H. Effect of different phosphorus supply levels on root characteristics and growth of alfalfa varieties with different phosphorus efficiency[D]. Urumqi:Xinjiang Agricultural University,2021:16-17 马红. 供磷水平对不同磷效率品种苜蓿生长和根系特征的影响[D]. 乌鲁木齐:新疆农业大学,2021:16-17
WEN Z H,PANG J Y,TUEUX G,et al. Contrasting patterns in biomass allocation,root morphology and mycorrhizal symbiosis for phosphorus acquisition among 20 chickpea genotypes with different amounts of rhizosheath carboxylates[J]. Functional Ecology,2020,34(7):1311-1324
WEN Z H,LI H B,SHEN Q,et al. Tradeoffs among root morphology,exudation and mycorrhizal symbioses for phosphorus-acquisition strategies of 16 crop species[J]. New Phytologist,2019,223(2):882-895
YANG C T,ZHANG Y Q,MA X X,et al. Screening genotypes and identifying indicators of different Fagopyrum tataricum varieties with low phosphorus tolerance[J]. Chinese Journal of Applied Ecology,2018,29(9):2997-3007 杨春婷,张永清,马星星,等.苦荞耐低磷基因型筛选及评价指标的鉴定[J]. 应用生态学报,2018,29(9):2997-3007
ZHANG J Y,HAN F,XIA L,et al. The response strategies of growth and root traits to phosphorus addition in four herbaceous plants[J]. Chinese Journal of Grassland,2023,45(1):43-52 张家铱,韩飞,夏蕾,等. 四种草本植物生长性状和根性状对磷添加的响应策略[J]. 中国草地学报,2023, 45(1):43-52
YAO R. Differences in acquisition strategies between different phosphorus efficient alfalfa genotypes[D]. Lanzhou:Lanzhou University,2023,15-17 姚瑞. 不同磷效率紫花苜蓿基因型的磷获取策略差异研究[D]. 兰州:兰州大学,2023,15-17
SHEN J B,YUAN L X,ZHANG J L,et al. Phosphorus dynamics: from soil to plant[J]. Plant Physiology,2011, 156(3):997-1005
CHEN Z C,LIAO H. Organic acid anions: an effective defensive weapon for plants against aluminum toxicity and phosphorus deficiency in acidic soils[J]. Journal of Genetics and Genomics,2016,43(11):631-638
PANG J Y,YANG J Y,LAMBERS H,et al. Physiological and morphological adaptations of herbaceous perennial legumes allow differential access to sources of varyingly soluble phosphate[J]. Physiologia Plantarum,2015,154(4):511-525
LI H,WANG C,ZHANG B B,et al. Trade-offs between root-secreted acid phosphatase and root morphology traits,and their contribution to phosphorus acquisition in Brassica napus[J]. Physiologia Plantarum,2024,176(2):e14247
ABEBE A T,ADEWALE S,CHIGEZA G,et al. Diallel analysis of soybean (Glycine max L.) for biomass yield and root characteristics under low phosphorus soil conditions in Western Ethiopia[J]. PLoS One,2023,18(2):e0281075
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WANG X X,LI H B,CHU Q,et al. Mycorrhizal impacts on root trait plasticity of six maize varieties along a phosphorus supply gradient[J]. Plant and Soil,2020,448(1):71-86
LAMBERS H,SHANE M W,CRAMER M D,et al. Root structure and functioning for efficient acquisition of phosphorus: matching morphological and physiological traits[J]. Annals of Botany,2006,98(4):693-713
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LI Z Y,ZHANG Q X,TONG Z Y,et al. Analysis of morphological and physiological responses to low pi stress in different alfalfas[J]. Scientia Agricultura Sinica,2017,50(20):3898-3907 栗振义,张绮芯,仝宗永,等. 不同紫花苜蓿品种对低磷环境的形态与生理响应分析[J]. 中国农业科学, 2017,50(20):3898-3907
REN L F,ZHANG W H,LI Y S. Effect of phosphorus deficiency on physiological properties of Medicago falcata[J]. Acta Prataculturae Sinica,2012,21(3):242-249 任立飞,张文浩,李衍素. 低磷胁迫对黄花苜蓿生理特性的影响[J]. 草业学报,2012,21(3):242-249
KONG D L,MA C G,ZHANG Q,et al. Leading dimensions in absorptive root trait variation across 96 subtropical forest species[J]. New Phytologist,2014,203(3):863-872)
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草地学报 |研究论文 2026 , 34 (2) : 392 -402
磷添加对不同品种苜蓿生物量及根系可塑性的影响
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冯恣萱1, 许紫莹1, 马红艳1, 荆晶莹1
作者信息
    1 中国农业大学草业科学与技术学院, 北京 100193
作者简介:
冯恣萱(2002-),女,汉族,四川成都人,硕士研究生,主要从事草地生产与利用研究,E-mail:15228827090@163.com
Effects of Phosphorus Addition on Biomass and Root Plasticity of Different Alfalfa Varieties
  • FENG Zi-xuan1, XU Zi-ying1, MA Hong-yan1, JING Jing-ying1
  • Affiliations
      1 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
    doi: 10.11733/j.issn.1007-0435.2026.02.002
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    为研究苜蓿根系形态的可塑性对不同磷供应强度的响应,选取30个不同的紫花苜蓿(Medicago sativa L.)品种和1个黄花苜蓿(Medicago falcata L.)品种,分别设置P₅(5 mg·kg⁻¹ KH₂PO₄)、P₂₀(20 mg·kg⁻¹ KH₂PO₄)和P₅₀(50 mg·kg⁻¹ KH₂PO₄)三个水平的磷添加处理,通过测定31个品种苜蓿的地上、地下生物量及根系形态指标,明确不同品种的苜蓿响应不同磷添加水平的差异,以期筛选出耐低磷的磷高效苜蓿品种。结果表明,P₅处理下苜蓿地上和地下生物量最低,随着供磷量的增加,苜蓿的地上生物量显著提高,在P₅₀处理下最高。在P₂₀和P₅₀处理下,苜蓿的根系生物量显著高于P₅处理,而根系形态在不同品种间差异显著。其中 ‘旱地’‘阿尔冈金’‘Magna Graze II’‘龙牧801’ 在低磷水平下较其他品种生物量高,且根长、根体积、根表面积以及根直径表现出更强的可塑性,为极耐低磷的磷高效品种。研究结果为苜蓿的合理栽培、磷肥利用率的提高提供科学依据。
    磷水平  /  苜蓿  /  根系形态  /  磷高效品种
    To investigate the responses of alfalfa root morphological traits to phosphorus supply, a total of 31 plant materials were selected comprising 30 varieties of Medicago sativa L. and one variety of Medicago falcata L., and three levels of phosphorus addition treatments were set up at P5 (5 mg·kg-1 KH2PO4), P20 (20 mg·kg-1 KH2PO4), and P50 (50 mg·kg-1 KH2PO4). To screen low phosphorus tolerant alfalfa varieties with high phosphorus use efficiency, we measured the aboveground and belowground biomass and root morphological indices for the 31 alfalfa varieties. The results indicated that the aboveground and belowground biomass of alfalfa were lowest under P5 treatment. With increasing phosphorus supply, the aboveground biomass of alfalfa significantly increased, reaching its highest level under the P50 treatment. Under the P20 and P50 treatments, the root biomass of alfalfa was significantly higher than that under P5, but there was no significant difference between P20 and P50. However, the root morphology showed significant variation among different varieties. Among them, ‘HD’‘Algonguin’‘Magna Graze II’ and ‘Longmu 801’ had higher biomass than other varieties, and exhibited greater root morphological plasticity with higher root length, root volume, root surface area, and root diameter, which demonstrated tolerance to low phosphorus condition. The results provide a scientific basis for optimizing alfalfa cultivation and improving phosphorus fertilizer utilization efficiency.
    Phosphorus level  /  Alfalfa  /  Root morphology  /  Phosphorus-efficient varieties
    冯恣萱, 许紫莹, 马红艳, 荆晶莹. 磷添加对不同品种苜蓿生物量及根系可塑性的影响. 草地学报, 2026 , 34 (2) : 392 -402 . DOI: 10.11733/j.issn.1007-0435.2026.02.002
    FENG Zi-xuan, XU Zi-ying, MA Hong-yan, JING Jing-ying. Effects of Phosphorus Addition on Biomass and Root Plasticity of Different Alfalfa Varieties[J]. Acta Agrestia Sinica, 2026 , 34 (2) : 392 -402 . DOI: 10.11733/j.issn.1007-0435.2026.02.002

      中法农业生态—农业绿色发展国际联合实验室项目 (2024YFE0105000)

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    2种不同金属材料的力学参数
    科
    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)
    属
    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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