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2 Department of Mining Engineering, Shanxi Institute of Technology, Yangquan, Shanxi Province 045000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309565391240786860, articleId=1309565391005905835, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=偏关苜蓿MsMYB44基因的克隆、亚细胞定位及表达分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=MYB（V-myb Avian myeloblastosis viral Oncogene Homolog）基因家族是植物中最大的转录因子家族之一，广泛参与植物的生长发育和逆境胁迫等过程。本研究以‘偏关’苜蓿（Medicago sativa ‘Pianguan’）为材料，通过基因克隆、生物信息学分析、亚细胞定位及表达模式分析来探究MsMYB44基因的特性。结果显示：‘偏关’苜蓿MsMYB44基因长966 bp，共编码321个氨基酸。MsMYB44蛋白与蒺藜苜蓿（Medicago truncatula）MtMYB44蛋白高度同源且定位于细胞核内。MsMYB44基因在‘偏关’苜蓿不同生育期的不同部位均有表达，表现出组织表达特异性。MsMYB44基因对干旱、盐胁迫及ABA处理均有响应。干旱胁迫下，MsMYB44相对表达量呈现出先升高后降低再升高的趋势，并在12 h时达到峰值；盐胁迫下，MsMYB44相对表达量在48 h后逐渐升高，且在72 h时达到最高值；ABA处理下，MsMYB44在12 h时相对表达量最高，随后逐渐降低。研究结果有望为阐释MsMYB44基因调控紫花苜蓿非生物胁迫响应机制提供一定理论参考。, authors=李迪娜¹, 王凯¹, 陶佳丽^1,2, 许涛¹, 李青秀¹, 朱慧森¹, 岑慧芳¹, authorsList=李迪娜, 王凯, 陶佳丽, 许涛, 李青秀, 朱慧森, 岑慧芳, authorCompany=1 山西农业大学草业学院, 山西 太谷 030801;
2 山西工程技术学院矿业工程系, 山西 阳泉 045000, correspAuthors=null, authorNote=李迪娜（1999-），女，汉族，黑龙江哈尔滨人，硕士研究生，主要从事牧草种质资源与育种相关研究，E-mail:13354593936@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=q32+0RJF7Hwnp2RrJqTwWA==, pdfFileSize=4577956, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目 (32071872); 山西省中央引导地方科技发展资金项目 (YDZJSX2022B006); 山西省重点研发计划课题 (202102140601006-3); 山西省基础研究计划 (202303021221101); 山西省来晋科研奖励 (SXBYKY2022118); 优秀博士启动项目 (2021BQ01,2023BQ03))}, authors=[Author(id=1320576666393498230, tenantId=1146029695717560320, journalId=null, articleId=1309565391005905835, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, 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